Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt holC and valS are separated by 15 nucleotides
SO3423: holC - DNA polymerase III, chi subunit (NCBI ptt file), at 3,563,011 to 3,563,475
holC
SO3424: valS - valyl-tRNA synthetase (NCBI ptt file), at 3,563,491 to 3,566,367
valS
Position (kb)
3563
3564 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3562.045 kb on - strand at 3562.076 kb on + strand at 3562.076 kb on + strand at 3562.097 kb on + strand at 3562.105 kb on - strand at 3562.105 kb on - strand at 3562.110 kb on - strand at 3562.140 kb on + strand at 3562.143 kb on + strand at 3562.153 kb on + strand at 3562.153 kb on + strand at 3562.161 kb on - strand at 3562.163 kb on + strand at 3562.241 kb on - strand at 3562.253 kb on + strand at 3562.253 kb on + strand at 3562.261 kb on - strand at 3562.265 kb on + strand at 3562.273 kb on - strand at 3562.319 kb on - strand at 3562.319 kb on - strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.336 kb on + strand at 3562.336 kb on + strand at 3562.336 kb on - strand at 3562.340 kb on + strand at 3562.340 kb on + strand at 3562.341 kb on - strand at 3562.341 kb on - strand at 3562.341 kb on - strand at 3562.341 kb on - strand at 3562.341 kb on - strand at 3562.341 kb on - strand at 3562.341 kb on - strand at 3562.343 kb on + strand at 3562.343 kb on + strand at 3562.344 kb on - strand at 3562.379 kb on + strand at 3562.379 kb on + strand at 3562.379 kb on + strand at 3562.387 kb on - strand at 3562.387 kb on - strand at 3562.387 kb on - strand at 3562.387 kb on - strand at 3562.387 kb on - strand at 3562.387 kb on - strand at 3562.387 kb on - strand at 3562.388 kb on + strand at 3562.389 kb on + strand at 3562.395 kb on - strand at 3562.395 kb on - strand at 3562.414 kb on - strand at 3562.424 kb on - strand at 3562.433 kb on - strand at 3562.433 kb on - strand at 3562.433 kb on - strand at 3562.442 kb on - strand at 3562.447 kb on - strand at 3562.450 kb on + strand at 3562.453 kb on - strand at 3562.528 kb on - strand at 3562.538 kb on - strand at 3562.556 kb on - strand at 3562.578 kb on - strand at 3562.578 kb on - strand at 3562.578 kb on - strand at 3562.604 kb on + strand at 3562.612 kb on - strand at 3562.612 kb on - strand at 3562.616 kb on + strand at 3562.616 kb on + strand at 3562.616 kb on + strand at 3562.624 kb on - strand at 3562.626 kb on + strand at 3562.626 kb on - strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.707 kb on - strand at 3562.709 kb on - strand at 3562.736 kb on + strand at 3562.736 kb on + strand at 3562.736 kb on + strand at 3562.736 kb on + strand at 3562.736 kb on + strand at 3562.736 kb on + strand at 3562.738 kb on + strand at 3562.738 kb on + strand at 3562.744 kb on - strand at 3562.744 kb on - strand at 3562.744 kb on - strand at 3562.744 kb on - strand at 3562.744 kb on - strand at 3562.746 kb on - strand at 3562.765 kb on + strand at 3562.774 kb on + strand at 3562.782 kb on + strand at 3562.806 kb on + strand at 3562.810 kb on + strand at 3562.810 kb on + strand at 3562.818 kb on - strand at 3562.818 kb on - strand at 3562.818 kb on - strand at 3562.818 kb on - strand at 3562.835 kb on + strand at 3562.837 kb on + strand at 3562.841 kb on + strand at 3562.842 kb on + strand at 3562.843 kb on + strand at 3562.850 kb on - strand at 3562.850 kb on - strand at 3562.850 kb on - strand at 3562.941 kb on + strand at 3562.941 kb on + strand at 3562.949 kb on - strand at 3562.949 kb on - strand at 3562.957 kb on + strand at 3562.957 kb on + strand at 3562.969 kb on + strand at 3562.975 kb on - strand at 3562.981 kb on + strand at 3562.988 kb on + strand at 3562.996 kb on - strand at 3562.996 kb on - strand at 3562.996 kb on - strand at 3562.996 kb on - strand at 3562.996 kb on - strand at 3562.996 kb on - strand at 3562.996 kb on - strand at 3562.997 kb on - strand at 3563.025 kb on + strand at 3563.025 kb on + strand at 3563.032 kb on - strand at 3563.032 kb on - strand at 3563.032 kb on - strand at 3563.033 kb on - strand at 3563.033 kb on - strand at 3563.033 kb on - strand at 3563.047 kb on + strand at 3563.059 kb on + strand, within holC at 3563.067 kb on - strand, within holC at 3563.067 kb on - strand, within holC at 3563.083 kb on + strand, within holC at 3563.124 kb on + strand, within holC at 3563.124 kb on + strand, within holC at 3563.132 kb on - strand, within holC at 3563.132 kb on - strand, within holC at 3563.132 kb on - strand, within holC at 3563.184 kb on + strand, within holC at 3563.206 kb on + strand, within holC at 3563.213 kb on + strand, within holC at 3563.229 kb on - strand, within holC at 3563.229 kb on - strand, within holC at 3563.229 kb on - strand, within holC at 3563.229 kb on - strand, within holC at 3563.268 kb on + strand, within holC
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 3,562,045 - +0.3 3,562,076 + +0.8 3,562,076 + +0.6 3,562,097 + +0.9 3,562,105 - +0.2 3,562,105 - +0.7 3,562,110 - -0.3 3,562,140 + +0.6 3,562,143 + +0.6 3,562,153 + -0.2 3,562,153 + -0.5 3,562,161 - +0.3 3,562,163 + +0.1 3,562,241 - +0.1 3,562,253 + -0.0 3,562,253 + +0.8 3,562,261 - +0.4 3,562,265 + +0.1 3,562,273 - +0.3 3,562,319 - +0.2 3,562,319 - +0.2 3,562,333 + -2.1 3,562,333 + +0.5 3,562,333 + +0.4 3,562,333 + +0.0 3,562,333 + +0.5 3,562,333 + -0.7 3,562,333 + -0.7 3,562,333 + -0.0 3,562,333 + -0.6 3,562,333 + +1.1 3,562,336 + +0.2 3,562,336 + -0.1 3,562,336 - -1.5 3,562,340 + -0.1 3,562,340 + +0.7 3,562,341 - +0.2 3,562,341 - +0.2 3,562,341 - +1.5 3,562,341 - +0.7 3,562,341 - +0.6 3,562,341 - +0.8 3,562,341 - +0.0 3,562,343 + +0.5 3,562,343 + +0.8 3,562,344 - -0.5 3,562,379 + -0.9 3,562,379 + -0.1 3,562,379 + +0.3 3,562,387 - +0.6 3,562,387 - +0.1 3,562,387 - +0.8 3,562,387 - -0.2 3,562,387 - +0.2 3,562,387 - -0.8 3,562,387 - -0.5 3,562,388 + +0.2 3,562,389 + +0.5 3,562,395 - +0.6 3,562,395 - +0.2 3,562,414 - -0.4 3,562,424 - +0.2 3,562,433 - +0.1 3,562,433 - +0.2 3,562,433 - +0.9 3,562,442 - -0.1 3,562,447 - -0.4 3,562,450 + +0.4 3,562,453 - +0.2 3,562,528 - -0.6 3,562,538 - -3.0 3,562,556 - -0.0 3,562,578 - -0.0 3,562,578 - +0.4 3,562,578 - +0.6 3,562,604 + -0.2 3,562,612 - +0.2 3,562,612 - +1.6 3,562,616 + +0.3 3,562,616 + -0.5 3,562,616 + +0.3 3,562,624 - +1.7 3,562,626 + -0.8 3,562,626 - +0.2 3,562,639 + -0.6 3,562,639 + +0.2 3,562,639 + -0.8 3,562,639 + +0.2 3,562,639 + +0.2 3,562,639 + +0.2 3,562,639 + -1.2 3,562,639 + +0.8 3,562,639 + -0.5 3,562,639 + -1.7 3,562,639 + +0.9 3,562,639 + -0.0 3,562,647 - -0.2 3,562,647 - +0.4 3,562,647 - +0.4 3,562,647 - -0.2 3,562,647 - +0.4 3,562,647 - +0.3 3,562,647 - +0.4 3,562,647 - +0.4 3,562,647 - -1.3 3,562,647 - -0.4 3,562,647 - -0.4 3,562,647 - +0.9 3,562,649 + +0.6 3,562,649 + -0.8 3,562,649 + +1.2 3,562,649 + -0.5 3,562,649 + -0.5 3,562,649 + +0.1 3,562,649 + +0.1 3,562,649 + -0.8 3,562,649 + -0.3 3,562,649 + -0.5 3,562,649 + +0.1 3,562,649 + -0.6 3,562,649 + +0.0 3,562,649 + -0.2 3,562,649 + +0.1 3,562,649 + +1.4 3,562,649 + +0.7 3,562,649 + -0.8 3,562,649 + -0.2 3,562,649 + +1.0 3,562,649 + +0.5 3,562,649 + +0.5 3,562,649 + +0.7 3,562,649 + -0.4 3,562,649 + -0.4 3,562,649 + -0.1 3,562,649 + +0.2 3,562,649 + +0.1 3,562,649 + +0.5 3,562,657 - +0.7 3,562,657 - -0.0 3,562,657 - +0.2 3,562,657 - -1.3 3,562,657 - +1.1 3,562,657 - -0.2 3,562,657 - +0.2 3,562,657 - +0.3 3,562,657 - +0.2 3,562,657 - +0.2 3,562,657 - -0.2 3,562,657 - -0.1 3,562,657 - +1.3 3,562,657 - +0.6 3,562,657 - -0.9 3,562,707 - +1.4 3,562,709 - -0.5 3,562,736 + +0.8 3,562,736 + -0.1 3,562,736 + +1.3 3,562,736 + +0.7 3,562,736 + +2.7 3,562,736 + +0.7 3,562,738 + +0.1 3,562,738 + +1.0 3,562,744 - +0.6 3,562,744 - +0.1 3,562,744 - -0.3 3,562,744 - +2.5 3,562,744 - +0.4 3,562,746 - +0.4 3,562,765 + +1.9 3,562,774 + +0.8 3,562,782 + -0.5 3,562,806 + +0.8 3,562,810 + +0.7 3,562,810 + +1.6 3,562,818 - +0.1 3,562,818 - -1.0 3,562,818 - -0.2 3,562,818 - +0.4 3,562,835 + +0.1 3,562,837 + -0.2 3,562,841 + -0.0 3,562,842 + -0.1 3,562,843 + -0.3 3,562,850 - -0.1 3,562,850 - +0.5 3,562,850 - -1.0 3,562,941 + +0.4 3,562,941 + -1.2 3,562,949 - -0.1 3,562,949 - -0.4 3,562,957 + -0.7 3,562,957 + -0.0 3,562,969 + +0.2 3,562,975 - -0.1 3,562,981 + -0.3 3,562,988 + -0.1 3,562,996 - -0.6 3,562,996 - -1.5 3,562,996 - +0.1 3,562,996 - +0.7 3,562,996 - +0.0 3,562,996 - -1.1 3,562,996 - -0.2 3,562,997 - -0.1 3,563,025 + -0.3 3,563,025 + -0.5 3,563,032 - +0.2 3,563,032 - +0.8 3,563,032 - -0.3 3,563,033 - -0.2 3,563,033 - +0.7 3,563,033 - -0.4 3,563,047 + -1.0 3,563,059 + holC SO3423 0.10 -0.3 3,563,067 - holC SO3423 0.12 -1.6 3,563,067 - holC SO3423 0.12 -0.0 3,563,083 + holC SO3423 0.15 +0.1 3,563,124 + holC SO3423 0.24 -0.6 3,563,124 + holC SO3423 0.24 -0.2 3,563,132 - holC SO3423 0.26 -0.9 3,563,132 - holC SO3423 0.26 -1.0 3,563,132 - holC SO3423 0.26 -0.3 3,563,184 + holC SO3423 0.37 -0.1 3,563,206 + holC SO3423 0.42 -0.5 3,563,213 + holC SO3423 0.43 +0.0 3,563,229 - holC SO3423 0.47 -0.6 3,563,229 - holC SO3423 0.47 -0.9 3,563,229 - holC SO3423 0.47 -0.0 3,563,229 - holC SO3423 0.47 -0.7 3,563,268 + holC SO3423 0.55 -1.6
Or see this region's nucleotide sequence