Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt deoB and deoD-2 are separated by 91 nucleotides deoD-2 and SO1222 are separated by 167 nucleotides
SO1219: deoB - Phosphopentomutase (EC 5.4.2.7) (from data) , at 1,266,092 to 1,267,306
deoB
SO1221: deoD-2 - purine nucleoside phosphorylase (NCBI ptt file), at 1,267,398 to 1,268,108
deoD-2
SO1222: SO1222 - hypothetical protein (NCBI ptt file), at 1,268,276 to 1,269,499
SO1222
Position (kb)
1267
1268
1269 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1266.485 kb on + strand, within deoB at 1266.495 kb on + strand, within deoB at 1266.554 kb on + strand, within deoB at 1266.706 kb on - strand, within deoB at 1266.876 kb on - strand, within deoB at 1266.876 kb on - strand, within deoB at 1266.912 kb on + strand, within deoB at 1267.025 kb on - strand, within deoB at 1267.093 kb on - strand, within deoB at 1267.139 kb on + strand, within deoB at 1267.149 kb on + strand, within deoB at 1267.157 kb on - strand, within deoB at 1267.157 kb on - strand, within deoB at 1267.186 kb on - strand at 1267.346 kb on - strand at 1267.346 kb on - strand at 1267.346 kb on - strand at 1267.346 kb on - strand at 1267.412 kb on + strand at 1267.479 kb on - strand, within deoD-2 at 1267.573 kb on - strand, within deoD-2 at 1267.687 kb on - strand, within deoD-2 at 1267.699 kb on - strand, within deoD-2 at 1267.724 kb on + strand, within deoD-2 at 1267.732 kb on - strand, within deoD-2 at 1267.807 kb on - strand, within deoD-2 at 1267.965 kb on + strand, within deoD-2 at 1268.038 kb on + strand at 1268.105 kb on + strand at 1268.113 kb on - strand at 1268.116 kb on + strand at 1268.116 kb on + strand at 1268.116 kb on + strand at 1268.116 kb on + strand at 1268.116 kb on + strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.351 kb on - strand at 1268.454 kb on + strand, within SO1222 at 1268.467 kb on - strand, within SO1222 at 1268.501 kb on + strand, within SO1222 at 1268.503 kb on + strand, within SO1222 at 1268.509 kb on - strand, within SO1222 at 1268.600 kb on - strand, within SO1222 at 1268.605 kb on + strand, within SO1222 at 1268.701 kb on - strand, within SO1222 at 1268.812 kb on + strand, within SO1222 at 1268.835 kb on + strand, within SO1222 at 1268.843 kb on - strand, within SO1222 at 1268.998 kb on - strand, within SO1222
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,266,485 + deoB SO1219 0.32 -0.4 1,266,495 + deoB SO1219 0.33 -0.2 1,266,554 + deoB SO1219 0.38 -0.6 1,266,706 - deoB SO1219 0.51 -0.0 1,266,876 - deoB SO1219 0.65 -0.5 1,266,876 - deoB SO1219 0.65 -0.1 1,266,912 + deoB SO1219 0.67 +0.1 1,267,025 - deoB SO1219 0.77 +0.2 1,267,093 - deoB SO1219 0.82 +1.5 1,267,139 + deoB SO1219 0.86 -0.4 1,267,149 + deoB SO1219 0.87 +0.3 1,267,157 - deoB SO1219 0.88 -0.6 1,267,157 - deoB SO1219 0.88 +0.7 1,267,186 - -0.6 1,267,346 - -1.2 1,267,346 - +0.4 1,267,346 - -2.5 1,267,346 - +0.7 1,267,412 + -0.8 1,267,479 - deoD-2 SO1221 0.11 -1.5 1,267,573 - deoD-2 SO1221 0.25 -1.8 1,267,687 - deoD-2 SO1221 0.41 -1.6 1,267,699 - deoD-2 SO1221 0.42 -1.5 1,267,724 + deoD-2 SO1221 0.46 -1.3 1,267,732 - deoD-2 SO1221 0.47 -1.6 1,267,807 - deoD-2 SO1221 0.58 -2.6 1,267,965 + deoD-2 SO1221 0.80 -1.8 1,268,038 + -1.2 1,268,105 + -0.2 1,268,113 - +0.6 1,268,116 + -0.4 1,268,116 + +1.9 1,268,116 + +0.5 1,268,116 + -0.0 1,268,116 + +0.2 1,268,124 - +1.3 1,268,124 - -0.0 1,268,124 - +0.5 1,268,124 - +0.4 1,268,124 - -0.0 1,268,124 - +0.7 1,268,124 - +0.9 1,268,124 - +0.6 1,268,124 - +1.3 1,268,124 - -0.0 1,268,124 - -0.6 1,268,124 - +0.7 1,268,351 - +0.0 1,268,454 + SO1222 0.15 +0.4 1,268,467 - SO1222 0.16 +0.0 1,268,501 + SO1222 0.18 -0.1 1,268,503 + SO1222 0.19 +0.6 1,268,509 - SO1222 0.19 +0.2 1,268,600 - SO1222 0.26 +1.2 1,268,605 + SO1222 0.27 -0.1 1,268,701 - SO1222 0.35 +0.8 1,268,812 + SO1222 0.44 +0.3 1,268,835 + SO1222 0.46 -0.9 1,268,843 - SO1222 0.46 -0.4 1,268,998 - SO1222 0.59 +1.0
Or see this region's nucleotide sequence