Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0965 and SO0966 overlap by 7 nucleotides SO0966 and SO0967 are separated by 66 nucleotides SO0967 and ldhA are separated by 67 nucleotides
SO0965: SO0965 - ISSod2, transposase OrfB (NCBI ptt file), at 998,573 to 999,388
SO0965
SO0966: SO0966 - ISSod2, transposase OrfA (NCBI ptt file), at 999,382 to 999,648
SO0966
SO0967: SO0967 - dienelactone hydrolase family protein (NCBI ptt file), at 999,715 to 1,000,233
SO0967
SO0968: ldhA - D-lactate dehydrogenase (NCBI ptt file), at 1,000,301 to 1,001,290
ldhA
Position (kb) 999
1000
1001 Strain fitness (log2 ratio) -2
-1
0
1 at 999.747 kb on - strand at 999.763 kb on + strand at 999.763 kb on + strand at 999.763 kb on + strand at 999.771 kb on - strand, within SO0967 at 999.771 kb on - strand, within SO0967 at 999.888 kb on + strand, within SO0967 at 999.929 kb on - strand, within SO0967 at 999.929 kb on - strand, within SO0967 at 999.953 kb on - strand, within SO0967 at 999.974 kb on - strand, within SO0967 at 1000.008 kb on + strand, within SO0967 at 1000.030 kb on - strand, within SO0967 at 1000.056 kb on + strand, within SO0967 at 1000.063 kb on + strand, within SO0967 at 1000.064 kb on - strand, within SO0967 at 1000.191 kb on - strand at 1000.243 kb on + strand at 1000.259 kb on + strand at 1000.273 kb on + strand at 1000.286 kb on + strand at 1000.394 kb on + strand at 1000.516 kb on + strand, within ldhA at 1000.596 kb on + strand, within ldhA at 1000.614 kb on - strand, within ldhA at 1000.614 kb on - strand, within ldhA at 1000.651 kb on + strand, within ldhA at 1000.666 kb on - strand, within ldhA at 1000.687 kb on - strand, within ldhA at 1000.728 kb on - strand, within ldhA at 1000.755 kb on + strand, within ldhA at 1000.755 kb on + strand, within ldhA at 1000.755 kb on + strand, within ldhA at 1000.763 kb on - strand, within ldhA at 1000.768 kb on + strand, within ldhA at 1000.768 kb on + strand, within ldhA at 1000.768 kb on + strand, within ldhA at 1000.776 kb on - strand, within ldhA at 1000.776 kb on - strand, within ldhA at 1000.776 kb on - strand, within ldhA at 1000.777 kb on - strand, within ldhA at 1000.807 kb on + strand, within ldhA at 1000.807 kb on + strand, within ldhA at 1001.026 kb on - strand, within ldhA at 1001.041 kb on + strand, within ldhA at 1001.051 kb on + strand, within ldhA at 1001.051 kb on + strand, within ldhA at 1001.059 kb on - strand, within ldhA at 1001.105 kb on + strand, within ldhA at 1001.186 kb on - strand, within ldhA
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 999,747 - -0.3 999,763 + -2.0 999,763 + +0.3 999,763 + -0.2 999,771 - SO0967 0.11 +0.5 999,771 - SO0967 0.11 +0.9 999,888 + SO0967 0.33 +0.1 999,929 - SO0967 0.41 +0.3 999,929 - SO0967 0.41 +0.4 999,953 - SO0967 0.46 +0.9 999,974 - SO0967 0.50 +0.2 1,000,008 + SO0967 0.56 +0.9 1,000,030 - SO0967 0.61 -0.1 1,000,056 + SO0967 0.66 +1.7 1,000,063 + SO0967 0.67 +0.1 1,000,064 - SO0967 0.67 -0.5 1,000,191 - -1.0 1,000,243 + -0.7 1,000,259 + -0.4 1,000,273 + +0.9 1,000,286 + +0.1 1,000,394 + -0.1 1,000,516 + ldhA SO0968 0.22 +0.3 1,000,596 + ldhA SO0968 0.30 -0.3 1,000,614 - ldhA SO0968 0.32 +0.9 1,000,614 - ldhA SO0968 0.32 -0.2 1,000,651 + ldhA SO0968 0.35 +0.0 1,000,666 - ldhA SO0968 0.37 -0.2 1,000,687 - ldhA SO0968 0.39 -1.0 1,000,728 - ldhA SO0968 0.43 -0.1 1,000,755 + ldhA SO0968 0.46 +0.4 1,000,755 + ldhA SO0968 0.46 -0.1 1,000,755 + ldhA SO0968 0.46 -0.1 1,000,763 - ldhA SO0968 0.47 -0.1 1,000,768 + ldhA SO0968 0.47 +0.1 1,000,768 + ldhA SO0968 0.47 +0.1 1,000,768 + ldhA SO0968 0.47 -0.2 1,000,776 - ldhA SO0968 0.48 +0.2 1,000,776 - ldhA SO0968 0.48 +0.7 1,000,776 - ldhA SO0968 0.48 +0.5 1,000,777 - ldhA SO0968 0.48 +0.9 1,000,807 + ldhA SO0968 0.51 -0.1 1,000,807 + ldhA SO0968 0.51 +0.1 1,001,026 - ldhA SO0968 0.73 +0.2 1,001,041 + ldhA SO0968 0.75 -0.5 1,001,051 + ldhA SO0968 0.76 -0.6 1,001,051 + ldhA SO0968 0.76 -0.0 1,001,059 - ldhA SO0968 0.77 +0.7 1,001,105 + ldhA SO0968 0.81 +0.9 1,001,186 - ldhA SO0968 0.89 -0.9
Or see this region's nucleotide sequence