Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hsdR-1 and SO0381 are separated by 269 nucleotides SO0381 and hsdS-1 are separated by 87 nucleotides
SO0380: hsdR-1 - type I restriction-modification system, R subunit (NCBI ptt file), at 391,526 to 394,627
hsdR-1
SO0381: SO0381 - death-on-curing family protein (NCBI ptt file), at 394,897 to 395,895
SO0381
SO0382: hsdS-1 - type I restriction-modification system, S subunit (NCBI ptt file), at 395,983 to 397,302
hsdS-1
Position (kb)
394
395
396 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 393.937 kb on + strand, within hsdR-1 at 393.953 kb on + strand, within hsdR-1 at 393.973 kb on - strand, within hsdR-1 at 393.975 kb on + strand, within hsdR-1 at 393.975 kb on + strand, within hsdR-1 at 393.978 kb on - strand, within hsdR-1 at 393.983 kb on - strand, within hsdR-1 at 393.983 kb on - strand, within hsdR-1 at 394.016 kb on - strand, within hsdR-1 at 394.036 kb on - strand, within hsdR-1 at 394.037 kb on - strand, within hsdR-1 at 394.125 kb on - strand, within hsdR-1 at 394.167 kb on + strand, within hsdR-1 at 394.236 kb on + strand, within hsdR-1 at 394.236 kb on + strand, within hsdR-1 at 394.254 kb on - strand, within hsdR-1 at 394.262 kb on + strand, within hsdR-1 at 394.264 kb on - strand, within hsdR-1 at 394.264 kb on - strand, within hsdR-1 at 394.269 kb on + strand, within hsdR-1 at 394.277 kb on - strand, within hsdR-1 at 394.277 kb on - strand, within hsdR-1 at 394.294 kb on + strand, within hsdR-1 at 394.294 kb on + strand, within hsdR-1 at 394.302 kb on - strand, within hsdR-1 at 394.302 kb on - strand, within hsdR-1 at 394.302 kb on - strand, within hsdR-1 at 394.309 kb on - strand, within hsdR-1 at 394.316 kb on + strand, within hsdR-1 at 394.316 kb on + strand, within hsdR-1 at 394.338 kb on + strand at 394.358 kb on + strand at 394.366 kb on - strand at 394.366 kb on - strand at 394.383 kb on - strand at 394.425 kb on + strand at 394.427 kb on - strand at 394.435 kb on + strand at 394.457 kb on - strand at 394.476 kb on + strand at 394.484 kb on - strand at 394.501 kb on - strand at 394.523 kb on - strand at 394.526 kb on + strand at 394.534 kb on - strand at 394.536 kb on - strand at 394.596 kb on + strand at 394.602 kb on + strand at 394.602 kb on + strand at 394.602 kb on + strand at 394.610 kb on - strand at 394.636 kb on - strand at 394.636 kb on - strand at 394.926 kb on + strand at 394.992 kb on - strand at 394.992 kb on - strand at 395.027 kb on + strand, within SO0381 at 395.029 kb on - strand, within SO0381 at 395.035 kb on - strand, within SO0381 at 395.187 kb on - strand, within SO0381 at 395.193 kb on - strand, within SO0381 at 395.219 kb on - strand, within SO0381 at 395.233 kb on - strand, within SO0381 at 395.331 kb on + strand, within SO0381 at 395.345 kb on - strand, within SO0381 at 395.406 kb on + strand, within SO0381 at 395.459 kb on + strand, within SO0381 at 395.511 kb on + strand, within SO0381 at 395.526 kb on + strand, within SO0381 at 395.526 kb on + strand, within SO0381 at 395.534 kb on - strand, within SO0381 at 395.534 kb on - strand, within SO0381 at 395.647 kb on + strand, within SO0381 at 395.648 kb on - strand, within SO0381 at 395.756 kb on - strand, within SO0381 at 395.756 kb on - strand, within SO0381 at 395.917 kb on + strand at 395.917 kb on + strand at 395.930 kb on - strand at 395.957 kb on + strand at 395.957 kb on + strand at 395.965 kb on - strand at 396.005 kb on + strand at 396.013 kb on - strand at 396.050 kb on + strand at 396.082 kb on - strand at 396.179 kb on - strand, within hsdS-1 at 396.185 kb on + strand, within hsdS-1 at 396.193 kb on - strand, within hsdS-1 at 396.296 kb on + strand, within hsdS-1 at 396.309 kb on - strand, within hsdS-1 at 396.318 kb on + strand, within hsdS-1 at 396.321 kb on - strand, within hsdS-1 at 396.323 kb on + strand, within hsdS-1 at 396.323 kb on + strand, within hsdS-1 at 396.323 kb on + strand, within hsdS-1 at 396.323 kb on + strand, within hsdS-1 at 396.323 kb on + strand, within hsdS-1 at 396.326 kb on - strand, within hsdS-1 at 396.330 kb on + strand, within hsdS-1 at 396.331 kb on - strand, within hsdS-1 at 396.331 kb on - strand, within hsdS-1 at 396.355 kb on + strand, within hsdS-1 at 396.363 kb on - strand, within hsdS-1 at 396.410 kb on + strand, within hsdS-1 at 396.421 kb on - strand, within hsdS-1 at 396.428 kb on + strand, within hsdS-1 at 396.428 kb on + strand, within hsdS-1 at 396.434 kb on + strand, within hsdS-1 at 396.436 kb on - strand, within hsdS-1 at 396.485 kb on + strand, within hsdS-1 at 396.501 kb on + strand, within hsdS-1 at 396.501 kb on + strand, within hsdS-1 at 396.502 kb on - strand, within hsdS-1 at 396.509 kb on - strand, within hsdS-1 at 396.509 kb on - strand, within hsdS-1 at 396.509 kb on - strand, within hsdS-1 at 396.509 kb on - strand, within hsdS-1 at 396.518 kb on - strand, within hsdS-1 at 396.536 kb on + strand, within hsdS-1 at 396.557 kb on + strand, within hsdS-1 at 396.559 kb on + strand, within hsdS-1 at 396.567 kb on + strand, within hsdS-1 at 396.572 kb on - strand, within hsdS-1 at 396.572 kb on - strand, within hsdS-1 at 396.574 kb on + strand, within hsdS-1 at 396.582 kb on - strand, within hsdS-1 at 396.596 kb on - strand, within hsdS-1 at 396.622 kb on - strand, within hsdS-1 at 396.668 kb on + strand, within hsdS-1 at 396.668 kb on + strand, within hsdS-1 at 396.668 kb on + strand, within hsdS-1 at 396.674 kb on + strand, within hsdS-1 at 396.676 kb on - strand, within hsdS-1 at 396.677 kb on + strand, within hsdS-1 at 396.685 kb on - strand, within hsdS-1 at 396.685 kb on - strand, within hsdS-1 at 396.721 kb on - strand, within hsdS-1 at 396.721 kb on - strand, within hsdS-1 at 396.766 kb on + strand, within hsdS-1 at 396.766 kb on + strand, within hsdS-1 at 396.774 kb on - strand, within hsdS-1 at 396.790 kb on - strand, within hsdS-1 at 396.793 kb on + strand, within hsdS-1 at 396.793 kb on + strand, within hsdS-1 at 396.804 kb on + strand, within hsdS-1 at 396.807 kb on - strand, within hsdS-1 at 396.856 kb on + strand, within hsdS-1 at 396.876 kb on + strand, within hsdS-1 at 396.884 kb on - strand, within hsdS-1
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 393,937 + hsdR-1 SO0380 0.78 +0.1 393,953 + hsdR-1 SO0380 0.78 -1.1 393,973 - hsdR-1 SO0380 0.79 -0.5 393,975 + hsdR-1 SO0380 0.79 -0.5 393,975 + hsdR-1 SO0380 0.79 -2.0 393,978 - hsdR-1 SO0380 0.79 +0.8 393,983 - hsdR-1 SO0380 0.79 +0.7 393,983 - hsdR-1 SO0380 0.79 -1.2 394,016 - hsdR-1 SO0380 0.80 -0.2 394,036 - hsdR-1 SO0380 0.81 -0.3 394,037 - hsdR-1 SO0380 0.81 +0.4 394,125 - hsdR-1 SO0380 0.84 -0.3 394,167 + hsdR-1 SO0380 0.85 -0.4 394,236 + hsdR-1 SO0380 0.87 +0.0 394,236 + hsdR-1 SO0380 0.87 +1.0 394,254 - hsdR-1 SO0380 0.88 -1.0 394,262 + hsdR-1 SO0380 0.88 -0.3 394,264 - hsdR-1 SO0380 0.88 +1.0 394,264 - hsdR-1 SO0380 0.88 +0.1 394,269 + hsdR-1 SO0380 0.88 -0.2 394,277 - hsdR-1 SO0380 0.89 -0.3 394,277 - hsdR-1 SO0380 0.89 +0.9 394,294 + hsdR-1 SO0380 0.89 +0.2 394,294 + hsdR-1 SO0380 0.89 +0.2 394,302 - hsdR-1 SO0380 0.89 +0.2 394,302 - hsdR-1 SO0380 0.89 +0.1 394,302 - hsdR-1 SO0380 0.89 -0.9 394,309 - hsdR-1 SO0380 0.90 -0.4 394,316 + hsdR-1 SO0380 0.90 -0.9 394,316 + hsdR-1 SO0380 0.90 +0.3 394,338 + -0.0 394,358 + +0.0 394,366 - -0.0 394,366 - -0.1 394,383 - -0.0 394,425 + +1.8 394,427 - -1.2 394,435 + -0.2 394,457 - -0.4 394,476 + -0.1 394,484 - -0.5 394,501 - +1.3 394,523 - -0.4 394,526 + +0.1 394,534 - -0.0 394,536 - +0.4 394,596 + -0.2 394,602 + +0.9 394,602 + +0.1 394,602 + +0.0 394,610 - -0.4 394,636 - +0.1 394,636 - -0.2 394,926 + -0.2 394,992 - +0.2 394,992 - -0.2 395,027 + SO0381 0.13 -0.5 395,029 - SO0381 0.13 +0.5 395,035 - SO0381 0.14 -0.1 395,187 - SO0381 0.29 -1.0 395,193 - SO0381 0.30 +1.3 395,219 - SO0381 0.32 +0.9 395,233 - SO0381 0.34 +0.3 395,331 + SO0381 0.43 -0.6 395,345 - SO0381 0.45 +0.0 395,406 + SO0381 0.51 +0.5 395,459 + SO0381 0.56 -0.6 395,511 + SO0381 0.61 +1.4 395,526 + SO0381 0.63 +0.5 395,526 + SO0381 0.63 -0.1 395,534 - SO0381 0.64 +0.4 395,534 - SO0381 0.64 -1.0 395,647 + SO0381 0.75 +0.7 395,648 - SO0381 0.75 +0.2 395,756 - SO0381 0.86 +0.3 395,756 - SO0381 0.86 -1.5 395,917 + -2.0 395,917 + -0.9 395,930 - -0.2 395,957 + -0.0 395,957 + +0.0 395,965 - +0.5 396,005 + +0.0 396,013 - +0.0 396,050 + -0.2 396,082 - -0.2 396,179 - hsdS-1 SO0382 0.15 +0.5 396,185 + hsdS-1 SO0382 0.15 +0.3 396,193 - hsdS-1 SO0382 0.16 +0.0 396,296 + hsdS-1 SO0382 0.24 +0.5 396,309 - hsdS-1 SO0382 0.25 +0.1 396,318 + hsdS-1 SO0382 0.25 +0.8 396,321 - hsdS-1 SO0382 0.26 +2.0 396,323 + hsdS-1 SO0382 0.26 -0.7 396,323 + hsdS-1 SO0382 0.26 +0.2 396,323 + hsdS-1 SO0382 0.26 -0.7 396,323 + hsdS-1 SO0382 0.26 +0.7 396,323 + hsdS-1 SO0382 0.26 -0.1 396,326 - hsdS-1 SO0382 0.26 -0.4 396,330 + hsdS-1 SO0382 0.26 -0.1 396,331 - hsdS-1 SO0382 0.26 +0.3 396,331 - hsdS-1 SO0382 0.26 +0.0 396,355 + hsdS-1 SO0382 0.28 -0.7 396,363 - hsdS-1 SO0382 0.29 -0.3 396,410 + hsdS-1 SO0382 0.32 -0.6 396,421 - hsdS-1 SO0382 0.33 -0.1 396,428 + hsdS-1 SO0382 0.34 -0.1 396,428 + hsdS-1 SO0382 0.34 -0.1 396,434 + hsdS-1 SO0382 0.34 -0.9 396,436 - hsdS-1 SO0382 0.34 -0.5 396,485 + hsdS-1 SO0382 0.38 +0.2 396,501 + hsdS-1 SO0382 0.39 -0.6 396,501 + hsdS-1 SO0382 0.39 -0.3 396,502 - hsdS-1 SO0382 0.39 +0.6 396,509 - hsdS-1 SO0382 0.40 +0.1 396,509 - hsdS-1 SO0382 0.40 -0.2 396,509 - hsdS-1 SO0382 0.40 +0.0 396,509 - hsdS-1 SO0382 0.40 +0.8 396,518 - hsdS-1 SO0382 0.41 -1.2 396,536 + hsdS-1 SO0382 0.42 -0.5 396,557 + hsdS-1 SO0382 0.43 +0.6 396,559 + hsdS-1 SO0382 0.44 -0.4 396,567 + hsdS-1 SO0382 0.44 -0.6 396,572 - hsdS-1 SO0382 0.45 +0.6 396,572 - hsdS-1 SO0382 0.45 +0.0 396,574 + hsdS-1 SO0382 0.45 -1.0 396,582 - hsdS-1 SO0382 0.45 +0.1 396,596 - hsdS-1 SO0382 0.46 -0.2 396,622 - hsdS-1 SO0382 0.48 +0.2 396,668 + hsdS-1 SO0382 0.52 -1.0 396,668 + hsdS-1 SO0382 0.52 +0.5 396,668 + hsdS-1 SO0382 0.52 -1.0 396,674 + hsdS-1 SO0382 0.52 -0.1 396,676 - hsdS-1 SO0382 0.53 -0.3 396,677 + hsdS-1 SO0382 0.53 -0.3 396,685 - hsdS-1 SO0382 0.53 -0.1 396,685 - hsdS-1 SO0382 0.53 +0.2 396,721 - hsdS-1 SO0382 0.56 +0.0 396,721 - hsdS-1 SO0382 0.56 -0.0 396,766 + hsdS-1 SO0382 0.59 +0.2 396,766 + hsdS-1 SO0382 0.59 +0.2 396,774 - hsdS-1 SO0382 0.60 -0.3 396,790 - hsdS-1 SO0382 0.61 -0.4 396,793 + hsdS-1 SO0382 0.61 +0.3 396,793 + hsdS-1 SO0382 0.61 +0.4 396,804 + hsdS-1 SO0382 0.62 +0.4 396,807 - hsdS-1 SO0382 0.62 +0.7 396,856 + hsdS-1 SO0382 0.66 +0.5 396,876 + hsdS-1 SO0382 0.68 -0.7 396,884 - hsdS-1 SO0382 0.68 +0.4
Or see this region's nucleotide sequence