Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroE and SO0041 are separated by 25 nucleotides SO0041 and SO0042 are separated by 91 nucleotides SO0042 and SO0043 are separated by 313 nucleotides SO0043 and Sp16SC are separated by 164 nucleotides
SO0040: aroE - shikimate 5-dehydrogenase (NCBI ptt file), at 43,717 to 44,580
aroE
SO0041: SO0041 - conserved hypothetical protein (NCBI ptt file), at 44,606 to 44,881
SO0041
SO0042: SO0042 - carbonic anhydrase, family 3 (NCBI ptt file), at 44,973 to 45,521
SO0042
SO0043: SO0043 - hypothetical protein (NCBI ptt file), at 45,835 to 45,951
SO0043
SO_r001: Sp16SC - 16S ribosomal RNA (RefSeq), at 46,116 to 47,644
Sp16SC
Position (kb) 44
45
46 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 43.983 kb on - strand, within aroE at 43.997 kb on + strand, within aroE at 43.997 kb on + strand, within aroE at 43.997 kb on + strand, within aroE at 43.998 kb on - strand, within aroE at 44.005 kb on - strand, within aroE at 44.005 kb on - strand, within aroE at 44.044 kb on + strand, within aroE at 44.045 kb on + strand, within aroE at 44.045 kb on + strand, within aroE at 44.052 kb on - strand, within aroE at 44.072 kb on - strand, within aroE at 44.072 kb on - strand, within aroE at 44.091 kb on + strand, within aroE at 44.099 kb on - strand, within aroE at 44.164 kb on - strand, within aroE at 44.196 kb on + strand, within aroE at 44.239 kb on - strand, within aroE at 44.239 kb on - strand, within aroE at 44.259 kb on + strand, within aroE at 44.279 kb on - strand, within aroE at 44.317 kb on + strand, within aroE at 44.347 kb on + strand, within aroE at 44.355 kb on - strand, within aroE at 44.380 kb on - strand, within aroE at 44.447 kb on + strand, within aroE at 44.449 kb on + strand, within aroE at 44.491 kb on + strand, within aroE at 44.491 kb on + strand, within aroE at 44.491 kb on + strand, within aroE at 44.491 kb on + strand, within aroE at 44.499 kb on - strand at 44.499 kb on - strand at 44.499 kb on - strand at 44.531 kb on - strand at 44.548 kb on - strand at 44.550 kb on + strand at 44.584 kb on + strand at 44.608 kb on - strand at 44.638 kb on + strand, within SO0041 at 44.638 kb on + strand, within SO0041 at 44.646 kb on - strand, within SO0041 at 44.752 kb on + strand, within SO0041 at 44.771 kb on + strand, within SO0041 at 44.797 kb on + strand, within SO0041 at 44.805 kb on - strand, within SO0041 at 44.870 kb on + strand at 44.871 kb on + strand at 44.925 kb on - strand at 44.933 kb on + strand at 44.947 kb on + strand at 44.955 kb on - strand at 44.955 kb on - strand at 45.068 kb on - strand, within SO0042 at 45.111 kb on + strand, within SO0042 at 45.126 kb on - strand, within SO0042 at 45.148 kb on + strand, within SO0042 at 45.148 kb on - strand, within SO0042 at 45.202 kb on - strand, within SO0042 at 45.224 kb on + strand, within SO0042 at 45.224 kb on + strand, within SO0042 at 45.224 kb on - strand, within SO0042 at 45.232 kb on - strand, within SO0042 at 45.232 kb on - strand, within SO0042 at 45.239 kb on + strand, within SO0042 at 45.239 kb on - strand, within SO0042 at 45.285 kb on - strand, within SO0042 at 45.336 kb on + strand, within SO0042 at 45.415 kb on + strand, within SO0042 at 45.420 kb on + strand, within SO0042 at 45.502 kb on + strand at 45.510 kb on - strand at 45.510 kb on - strand at 45.510 kb on - strand at 45.700 kb on + strand at 45.732 kb on - strand at 45.801 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 43,983 - aroE SO0040 0.31 -2.3 43,997 + aroE SO0040 0.32 -2.0 43,997 + aroE SO0040 0.32 -4.6 43,997 + aroE SO0040 0.32 -2.9 43,998 - aroE SO0040 0.33 -3.7 44,005 - aroE SO0040 0.33 -2.5 44,005 - aroE SO0040 0.33 -1.3 44,044 + aroE SO0040 0.38 -3.9 44,045 + aroE SO0040 0.38 -1.4 44,045 + aroE SO0040 0.38 -4.5 44,052 - aroE SO0040 0.39 -3.9 44,072 - aroE SO0040 0.41 -4.0 44,072 - aroE SO0040 0.41 -4.7 44,091 + aroE SO0040 0.43 -3.9 44,099 - aroE SO0040 0.44 -2.1 44,164 - aroE SO0040 0.52 -5.1 44,196 + aroE SO0040 0.55 -3.6 44,239 - aroE SO0040 0.60 -1.4 44,239 - aroE SO0040 0.60 -1.5 44,259 + aroE SO0040 0.63 -2.3 44,279 - aroE SO0040 0.65 -2.7 44,317 + aroE SO0040 0.69 -4.3 44,347 + aroE SO0040 0.73 -4.2 44,355 - aroE SO0040 0.74 -5.0 44,380 - aroE SO0040 0.77 -3.9 44,447 + aroE SO0040 0.84 -2.7 44,449 + aroE SO0040 0.85 -2.3 44,491 + aroE SO0040 0.90 -1.4 44,491 + aroE SO0040 0.90 -3.7 44,491 + aroE SO0040 0.90 -4.9 44,491 + aroE SO0040 0.90 -3.0 44,499 - -3.5 44,499 - -4.7 44,499 - -3.1 44,531 - -3.8 44,548 - -2.2 44,550 + -0.3 44,584 + -0.2 44,608 - -0.5 44,638 + SO0041 0.12 -0.1 44,638 + SO0041 0.12 +0.2 44,646 - SO0041 0.14 -0.5 44,752 + SO0041 0.53 -0.3 44,771 + SO0041 0.60 +0.6 44,797 + SO0041 0.69 -0.3 44,805 - SO0041 0.72 -0.1 44,870 + -0.3 44,871 + +0.1 44,925 - -0.8 44,933 + -0.2 44,947 + +0.5 44,955 - -0.1 44,955 - +0.3 45,068 - SO0042 0.17 -0.3 45,111 + SO0042 0.25 +0.1 45,126 - SO0042 0.28 +0.3 45,148 + SO0042 0.32 -1.5 45,148 - SO0042 0.32 -1.2 45,202 - SO0042 0.42 -2.0 45,224 + SO0042 0.46 -0.3 45,224 + SO0042 0.46 -0.5 45,224 - SO0042 0.46 -0.9 45,232 - SO0042 0.47 -1.3 45,232 - SO0042 0.47 -1.1 45,239 + SO0042 0.48 -0.2 45,239 - SO0042 0.48 -1.0 45,285 - SO0042 0.57 -0.0 45,336 + SO0042 0.66 -2.1 45,415 + SO0042 0.81 -1.5 45,420 + SO0042 0.81 -1.3 45,502 + -1.0 45,510 - -0.5 45,510 - -0.4 45,510 - -2.7 45,700 + +0.4 45,732 - -0.2 45,801 + +0.2
Or see this region's nucleotide sequence