Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4475 and SO4476 are separated by 50 nucleotides SO4476 and cpxR are separated by 343 nucleotides cpxR and cpxA are separated by 14 nucleotides
SO4475: SO4475 - cation efflux family protein (NCBI ptt file), at 4,663,350 to 4,664,240
SO4475
SO4476: SO4476 - spheroplast protein y precursor, putative (NCBI ptt file), at 4,664,291 to 4,664,782
SO4476
SO4477: cpxR - transcriptional regulatory protein CpxR (NCBI ptt file), at 4,665,126 to 4,665,812
cpxR
SO4478: cpxA - sensor protein CpxA (NCBI ptt file), at 4,665,827 to 4,667,176
cpxA
Position (kb)
4665
4666 Strain fitness (log2 ratio)
-1
0
1 at 4664.168 kb on + strand at 4664.169 kb on + strand at 4664.259 kb on + strand at 4664.259 kb on + strand at 4664.267 kb on - strand at 4664.279 kb on - strand at 4664.321 kb on - strand at 4664.327 kb on - strand at 4664.435 kb on + strand, within SO4476 at 4664.435 kb on + strand, within SO4476 at 4664.467 kb on + strand, within SO4476 at 4664.511 kb on + strand, within SO4476 at 4664.599 kb on + strand, within SO4476 at 4664.603 kb on - strand, within SO4476 at 4664.644 kb on - strand, within SO4476 at 4664.684 kb on + strand, within SO4476 at 4664.688 kb on + strand, within SO4476 at 4664.698 kb on - strand, within SO4476 at 4664.701 kb on - strand, within SO4476 at 4664.703 kb on - strand, within SO4476 at 4664.709 kb on + strand, within SO4476 at 4664.717 kb on - strand, within SO4476 at 4664.717 kb on - strand at 4664.719 kb on + strand, within SO4476 at 4664.764 kb on + strand at 4664.799 kb on - strand at 4664.869 kb on + strand at 4664.869 kb on + strand at 4664.877 kb on + strand at 4664.877 kb on - strand at 4664.877 kb on - strand at 4664.904 kb on + strand at 4664.904 kb on + strand at 4664.904 kb on + strand at 4664.904 kb on + strand at 4664.904 kb on + strand at 4664.912 kb on - strand at 4664.922 kb on - strand at 4664.952 kb on + strand at 4665.002 kb on + strand at 4665.045 kb on - strand at 4665.093 kb on + strand at 4665.101 kb on - strand at 4665.101 kb on - strand at 4665.112 kb on + strand at 4665.120 kb on - strand at 4665.208 kb on - strand, within cpxR at 4665.223 kb on - strand, within cpxR at 4665.288 kb on - strand, within cpxR at 4665.288 kb on - strand, within cpxR at 4665.288 kb on - strand, within cpxR at 4665.288 kb on - strand, within cpxR at 4665.288 kb on - strand, within cpxR at 4665.304 kb on + strand, within cpxR at 4665.304 kb on + strand, within cpxR at 4665.304 kb on + strand, within cpxR at 4665.312 kb on - strand, within cpxR at 4665.312 kb on - strand, within cpxR at 4665.312 kb on - strand, within cpxR at 4665.314 kb on + strand, within cpxR at 4665.416 kb on + strand, within cpxR at 4665.538 kb on + strand, within cpxR at 4665.600 kb on + strand, within cpxR at 4665.609 kb on - strand, within cpxR at 4665.611 kb on + strand, within cpxR at 4665.619 kb on + strand, within cpxR at 4665.619 kb on + strand, within cpxR at 4665.619 kb on + strand, within cpxR at 4665.619 kb on + strand, within cpxR at 4665.627 kb on - strand, within cpxR at 4665.627 kb on - strand, within cpxR at 4665.627 kb on - strand, within cpxR at 4665.627 kb on - strand, within cpxR at 4665.714 kb on - strand, within cpxR at 4665.749 kb on + strand at 4665.792 kb on + strand at 4665.792 kb on + strand at 4665.800 kb on - strand at 4665.800 kb on - strand at 4665.800 kb on - strand at 4665.800 kb on - strand at 4665.800 kb on - strand at 4665.800 kb on - strand at 4665.936 kb on + strand at 4666.004 kb on - strand, within cpxA at 4666.004 kb on - strand, within cpxA at 4666.014 kb on - strand, within cpxA at 4666.014 kb on - strand, within cpxA at 4666.076 kb on - strand, within cpxA at 4666.119 kb on + strand, within cpxA at 4666.125 kb on - strand, within cpxA at 4666.127 kb on - strand, within cpxA at 4666.127 kb on - strand, within cpxA at 4666.134 kb on + strand, within cpxA at 4666.142 kb on - strand, within cpxA at 4666.142 kb on - strand, within cpxA at 4666.180 kb on + strand, within cpxA at 4666.255 kb on + strand, within cpxA at 4666.319 kb on - strand, within cpxA at 4666.337 kb on + strand, within cpxA at 4666.337 kb on + strand, within cpxA at 4666.337 kb on + strand, within cpxA at 4666.337 kb on + strand, within cpxA at 4666.342 kb on + strand, within cpxA at 4666.345 kb on - strand, within cpxA at 4666.369 kb on + strand, within cpxA at 4666.574 kb on + strand, within cpxA at 4666.584 kb on + strand, within cpxA at 4666.584 kb on + strand, within cpxA at 4666.783 kb on + strand, within cpxA at 4666.802 kb on + strand, within cpxA
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 4,664,168 + -0.5 4,664,169 + -0.3 4,664,259 + -0.0 4,664,259 + -0.7 4,664,267 - -0.4 4,664,279 - -0.1 4,664,321 - -1.1 4,664,327 - +1.6 4,664,435 + SO4476 0.29 +0.5 4,664,435 + SO4476 0.29 +0.2 4,664,467 + SO4476 0.36 +0.4 4,664,511 + SO4476 0.45 +0.1 4,664,599 + SO4476 0.63 +0.1 4,664,603 - SO4476 0.63 +0.2 4,664,644 - SO4476 0.72 -0.2 4,664,684 + SO4476 0.80 -0.9 4,664,688 + SO4476 0.81 +0.3 4,664,698 - SO4476 0.83 +1.0 4,664,701 - SO4476 0.83 -0.5 4,664,703 - SO4476 0.84 -0.2 4,664,709 + SO4476 0.85 +0.5 4,664,717 - SO4476 0.87 +0.3 4,664,717 - +0.9 4,664,719 + SO4476 0.87 -0.2 4,664,764 + -0.2 4,664,799 - +1.3 4,664,869 + +0.8 4,664,869 + +0.9 4,664,877 + -0.5 4,664,877 - +0.5 4,664,877 - +0.6 4,664,904 + +0.5 4,664,904 + -0.8 4,664,904 + +0.5 4,664,904 + +1.0 4,664,904 + +0.5 4,664,912 - +0.1 4,664,922 - -0.2 4,664,952 + +0.9 4,665,002 + -0.2 4,665,045 - +1.2 4,665,093 + +1.2 4,665,101 - +0.3 4,665,101 - -0.0 4,665,112 + -1.1 4,665,120 - -0.6 4,665,208 - cpxR SO4477 0.12 +0.3 4,665,223 - cpxR SO4477 0.14 +0.9 4,665,288 - cpxR SO4477 0.24 +0.6 4,665,288 - cpxR SO4477 0.24 -0.1 4,665,288 - cpxR SO4477 0.24 +1.1 4,665,288 - cpxR SO4477 0.24 -0.9 4,665,288 - cpxR SO4477 0.24 -0.1 4,665,304 + cpxR SO4477 0.26 +0.6 4,665,304 + cpxR SO4477 0.26 +0.5 4,665,304 + cpxR SO4477 0.26 +0.3 4,665,312 - cpxR SO4477 0.27 +1.4 4,665,312 - cpxR SO4477 0.27 +1.1 4,665,312 - cpxR SO4477 0.27 +0.7 4,665,314 + cpxR SO4477 0.27 +0.7 4,665,416 + cpxR SO4477 0.42 +0.6 4,665,538 + cpxR SO4477 0.60 +0.2 4,665,600 + cpxR SO4477 0.69 +1.1 4,665,609 - cpxR SO4477 0.70 +0.9 4,665,611 + cpxR SO4477 0.71 +0.1 4,665,619 + cpxR SO4477 0.72 +0.4 4,665,619 + cpxR SO4477 0.72 +1.6 4,665,619 + cpxR SO4477 0.72 +0.1 4,665,619 + cpxR SO4477 0.72 +0.4 4,665,627 - cpxR SO4477 0.73 +0.8 4,665,627 - cpxR SO4477 0.73 +0.6 4,665,627 - cpxR SO4477 0.73 +0.2 4,665,627 - cpxR SO4477 0.73 +0.2 4,665,714 - cpxR SO4477 0.86 +0.2 4,665,749 + +0.8 4,665,792 + +0.2 4,665,792 + +1.2 4,665,800 - -1.3 4,665,800 - +0.7 4,665,800 - +0.6 4,665,800 - +0.4 4,665,800 - +1.4 4,665,800 - +0.4 4,665,936 + +0.7 4,666,004 - cpxA SO4478 0.13 +0.4 4,666,004 - cpxA SO4478 0.13 +0.7 4,666,014 - cpxA SO4478 0.14 -0.1 4,666,014 - cpxA SO4478 0.14 +0.5 4,666,076 - cpxA SO4478 0.18 +0.2 4,666,119 + cpxA SO4478 0.22 -0.2 4,666,125 - cpxA SO4478 0.22 +0.4 4,666,127 - cpxA SO4478 0.22 +0.5 4,666,127 - cpxA SO4478 0.22 +0.1 4,666,134 + cpxA SO4478 0.23 +0.8 4,666,142 - cpxA SO4478 0.23 -0.0 4,666,142 - cpxA SO4478 0.23 +1.1 4,666,180 + cpxA SO4478 0.26 +0.1 4,666,255 + cpxA SO4478 0.32 +0.3 4,666,319 - cpxA SO4478 0.36 -0.2 4,666,337 + cpxA SO4478 0.38 +0.8 4,666,337 + cpxA SO4478 0.38 +1.0 4,666,337 + cpxA SO4478 0.38 +0.2 4,666,337 + cpxA SO4478 0.38 +1.2 4,666,342 + cpxA SO4478 0.38 +1.2 4,666,345 - cpxA SO4478 0.38 -1.0 4,666,369 + cpxA SO4478 0.40 +0.6 4,666,574 + cpxA SO4478 0.55 +0.2 4,666,584 + cpxA SO4478 0.56 -0.8 4,666,584 + cpxA SO4478 0.56 +0.4 4,666,783 + cpxA SO4478 0.71 +0.2 4,666,802 + cpxA SO4478 0.72 -0.5
Or see this region's nucleotide sequence