Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4303 and SO4304 are separated by 5 nucleotides SO4304 and SO4305 are separated by 125 nucleotides SO4305 and xerC overlap by 1 nucleotides xerC and SO4307 are separated by 41 nucleotides
SO4303: SO4303 - hypothetical protein (NCBI ptt file), at 4,481,686 to 4,482,189
SO4303
SO4304: SO4304 - conserved hypothetical protein (NCBI ptt file), at 4,482,195 to 4,482,770
SO4304
SO4305: SO4305 - HAD-superfamily hydrolase, subfamily IA, variant 1 family protein (NCBI ptt file), at 4,482,896 to 4,483,618
SO4305
SO4306: xerC - integrase/recombinase XerC (NCBI ptt file), at 4,483,618 to 4,484,517
xerC
SO4307: SO4307 - conserved hypothetical protein (NCBI ptt file), at 4,484,559 to 4,485,179
SO4307
Position (kb)
4482
4483
4484 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4481.937 kb on - strand, within SO4303 at 4482.035 kb on + strand, within SO4303 at 4482.038 kb on - strand, within SO4303 at 4482.145 kb on - strand at 4482.164 kb on + strand at 4482.172 kb on - strand at 4482.256 kb on + strand, within SO4304 at 4482.259 kb on - strand, within SO4304 at 4482.304 kb on - strand, within SO4304 at 4482.304 kb on - strand, within SO4304 at 4482.389 kb on + strand, within SO4304 at 4482.389 kb on + strand, within SO4304 at 4482.544 kb on - strand, within SO4304 at 4482.623 kb on + strand, within SO4304 at 4482.671 kb on + strand, within SO4304 at 4482.672 kb on + strand, within SO4304 at 4482.679 kb on - strand, within SO4304 at 4482.723 kb on - strand at 4482.838 kb on + strand at 4482.846 kb on - strand at 4482.861 kb on + strand at 4482.947 kb on + strand at 4482.947 kb on + strand at 4482.955 kb on + strand at 4482.955 kb on - strand at 4482.972 kb on + strand, within SO4305 at 4483.044 kb on + strand, within SO4305 at 4483.075 kb on + strand, within SO4305 at 4483.083 kb on - strand, within SO4305 at 4483.136 kb on - strand, within SO4305 at 4483.261 kb on - strand, within SO4305 at 4483.347 kb on - strand, within SO4305 at 4483.350 kb on + strand, within SO4305 at 4483.363 kb on - strand, within SO4305 at 4483.568 kb on - strand at 4483.600 kb on + strand at 4483.657 kb on + strand at 4483.666 kb on - strand at 4483.825 kb on + strand, within xerC at 4483.882 kb on - strand, within xerC at 4483.931 kb on - strand, within xerC at 4483.997 kb on + strand, within xerC at 4484.005 kb on - strand, within xerC at 4484.070 kb on + strand, within xerC at 4484.195 kb on + strand, within xerC at 4484.195 kb on + strand, within xerC at 4484.195 kb on + strand, within xerC at 4484.319 kb on + strand, within xerC at 4484.469 kb on + strand at 4484.500 kb on - strand at 4484.521 kb on - strand at 4484.521 kb on - strand at 4484.521 kb on - strand at 4484.521 kb on - strand at 4484.547 kb on + strand at 4484.581 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 4,481,937 - SO4303 0.50 +0.4 4,482,035 + SO4303 0.69 +0.6 4,482,038 - SO4303 0.70 +0.5 4,482,145 - +0.3 4,482,164 + -0.1 4,482,172 - +0.9 4,482,256 + SO4304 0.11 +0.8 4,482,259 - SO4304 0.11 -0.1 4,482,304 - SO4304 0.19 -0.1 4,482,304 - SO4304 0.19 -0.0 4,482,389 + SO4304 0.34 -1.1 4,482,389 + SO4304 0.34 -0.2 4,482,544 - SO4304 0.61 +0.1 4,482,623 + SO4304 0.74 -0.2 4,482,671 + SO4304 0.83 -0.9 4,482,672 + SO4304 0.83 +0.2 4,482,679 - SO4304 0.84 +0.1 4,482,723 - -0.1 4,482,838 + -1.9 4,482,846 - -0.2 4,482,861 + -1.0 4,482,947 + +2.2 4,482,947 + +2.6 4,482,955 + +2.9 4,482,955 - +3.1 4,482,972 + SO4305 0.11 +3.1 4,483,044 + SO4305 0.20 +3.3 4,483,075 + SO4305 0.25 +2.2 4,483,083 - SO4305 0.26 +3.1 4,483,136 - SO4305 0.33 +3.5 4,483,261 - SO4305 0.50 +2.4 4,483,347 - SO4305 0.62 +3.2 4,483,350 + SO4305 0.63 +2.0 4,483,363 - SO4305 0.65 +2.9 4,483,568 - +4.3 4,483,600 + +2.9 4,483,657 + -2.1 4,483,666 - +0.2 4,483,825 + xerC SO4306 0.23 -0.4 4,483,882 - xerC SO4306 0.29 -0.1 4,483,931 - xerC SO4306 0.35 -1.3 4,483,997 + xerC SO4306 0.42 -0.9 4,484,005 - xerC SO4306 0.43 -0.9 4,484,070 + xerC SO4306 0.50 -1.5 4,484,195 + xerC SO4306 0.64 -1.0 4,484,195 + xerC SO4306 0.64 -0.8 4,484,195 + xerC SO4306 0.64 -1.5 4,484,319 + xerC SO4306 0.78 -1.6 4,484,469 + -0.6 4,484,500 - -0.3 4,484,521 - +1.2 4,484,521 - +0.3 4,484,521 - -0.3 4,484,521 - -1.1 4,484,547 + -0.2 4,484,581 - +0.4
Or see this region's nucleotide sequence