Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_3150

Experiment: Def sodium fluoride 25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_3145 and Psest_3146 overlap by 4 nucleotidesPsest_3146 and Psest_3147 overlap by 1 nucleotidesPsest_3147 and Psest_3148 overlap by 20 nucleotidesPsest_3148 and Psest_3149 are separated by 10 nucleotidesPsest_3149 and Psest_3150 overlap by 32 nucleotidesPsest_3150 and Psest_3151 are separated by 319 nucleotides Psest_3145: Psest_3145 - hypothetical protein, at 3,319,351 to 3,319,635 _3145 Psest_3146: Psest_3146 - hypothetical protein, at 3,319,632 to 3,319,844 _3146 Psest_3147: Psest_3147 - hypothetical protein, at 3,319,844 to 3,320,017 _3147 Psest_3148: Psest_3148 - hypothetical protein, at 3,319,998 to 3,320,177 _3148 Psest_3149: Psest_3149 - hypothetical protein, at 3,320,188 to 3,320,370 _3149 Psest_3150: Psest_3150 - hypothetical protein, at 3,320,339 to 3,321,598 _3150 Psest_3151: Psest_3151 - hypothetical protein, at 3,321,918 to 3,322,388 _3151 Position (kb) 3320 3321 3322Strain fitness (log2 ratio) -2 -1 0 1at 3319.349 kb on + strandat 3319.454 kb on - strand, within Psest_3145at 3319.587 kb on - strand, within Psest_3145at 3319.689 kb on - strand, within Psest_3146at 3319.768 kb on + strand, within Psest_3146at 3319.793 kb on - strand, within Psest_3146at 3319.827 kb on - strandat 3319.827 kb on - strandat 3319.827 kb on - strandat 3320.088 kb on - strand, within Psest_3148at 3320.461 kb on + strandat 3320.540 kb on + strand, within Psest_3150at 3320.541 kb on - strand, within Psest_3150at 3320.638 kb on + strand, within Psest_3150at 3320.665 kb on + strand, within Psest_3150at 3320.666 kb on - strand, within Psest_3150at 3320.679 kb on + strand, within Psest_3150at 3320.701 kb on - strand, within Psest_3150at 3320.736 kb on - strand, within Psest_3150at 3320.802 kb on + strand, within Psest_3150at 3320.904 kb on + strand, within Psest_3150at 3320.904 kb on + strand, within Psest_3150at 3320.925 kb on + strand, within Psest_3150at 3320.981 kb on - strand, within Psest_3150at 3321.037 kb on + strand, within Psest_3150at 3321.080 kb on - strand, within Psest_3150at 3321.194 kb on - strand, within Psest_3150at 3321.242 kb on + strand, within Psest_3150at 3321.363 kb on + strand, within Psest_3150at 3321.364 kb on - strand, within Psest_3150at 3321.391 kb on + strand, within Psest_3150at 3321.514 kb on + strandat 3321.514 kb on + strandat 3321.514 kb on + strandat 3321.597 kb on - strandat 3321.649 kb on - strandat 3321.675 kb on - strandat 3321.675 kb on - strandat 3321.698 kb on - strandat 3321.932 kb on - strandat 3322.140 kb on + strand, within Psest_3151at 3322.141 kb on - strand, within Psest_3151at 3322.143 kb on - strand, within Psest_3151at 3322.181 kb on + strand, within Psest_3151at 3322.269 kb on + strand, within Psest_3151at 3322.386 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Def sodium fluoride 25 mM
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3,319,349 + -1.7
3,319,454 - Psest_3145 0.36 +0.2
3,319,587 - Psest_3145 0.83 +0.2
3,319,689 - Psest_3146 0.27 +0.1
3,319,768 + Psest_3146 0.64 +0.4
3,319,793 - Psest_3146 0.76 -0.1
3,319,827 - +0.9
3,319,827 - -0.6
3,319,827 - +0.1
3,320,088 - Psest_3148 0.50 -0.2
3,320,461 + -0.1
3,320,540 + Psest_3150 0.16 +0.0
3,320,541 - Psest_3150 0.16 +0.3
3,320,638 + Psest_3150 0.24 +1.3
3,320,665 + Psest_3150 0.26 +1.0
3,320,666 - Psest_3150 0.26 +0.6
3,320,679 + Psest_3150 0.27 +0.1
3,320,701 - Psest_3150 0.29 +1.0
3,320,736 - Psest_3150 0.32 -2.2
3,320,802 + Psest_3150 0.37 +0.2
3,320,904 + Psest_3150 0.45 +0.9
3,320,904 + Psest_3150 0.45 +0.5
3,320,925 + Psest_3150 0.47 +0.5
3,320,981 - Psest_3150 0.51 -0.7
3,321,037 + Psest_3150 0.55 +0.8
3,321,080 - Psest_3150 0.59 +0.1
3,321,194 - Psest_3150 0.68 +0.8
3,321,242 + Psest_3150 0.72 +0.8
3,321,363 + Psest_3150 0.81 -0.0
3,321,364 - Psest_3150 0.81 -0.8
3,321,391 + Psest_3150 0.83 -1.1
3,321,514 + -0.1
3,321,514 + -0.6
3,321,514 + -0.2
3,321,597 - -0.0
3,321,649 - +0.1
3,321,675 - -0.6
3,321,675 - -0.7
3,321,698 - -0.4
3,321,932 - -0.0
3,322,140 + Psest_3151 0.47 -0.1
3,322,141 - Psest_3151 0.47 +0.4
3,322,143 - Psest_3151 0.48 -1.1
3,322,181 + Psest_3151 0.56 +0.3
3,322,269 + Psest_3151 0.75 -0.2
3,322,386 + -2.5

Or see this region's nucleotide sequence