Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_3008

Experiment: Def sodium fluoride 25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_3006 and Psest_3007 overlap by 1 nucleotidesPsest_3007 and Psest_3008 are separated by 2 nucleotidesPsest_3008 and Psest_3009 overlap by 4 nucleotidesPsest_3009 and Psest_3010 overlap by 8 nucleotides Psest_3006: Psest_3006 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 3,194,548 to 3,195,597 _3006 Psest_3007: Psest_3007 - Adenosyl cobinamide kinase/adenosyl cobinamide phosphate guanylyltransferase, at 3,195,597 to 3,196,115 _3007 Psest_3008: Psest_3008 - cobyric acid synthase CobQ, at 3,196,118 to 3,197,575 _3008 Psest_3009: Psest_3009 - L-threonine-O-3-phosphate decarboxylase, at 3,197,572 to 3,198,561 _3009 Psest_3010: Psest_3010 - cobalamin biosynthesis protein CobD, at 3,198,554 to 3,199,465 _3010 Position (kb) 3196 3197 3198Strain fitness (log2 ratio) -2 -1 0 1 2at 3195.420 kb on + strand, within Psest_3006at 3195.420 kb on + strand, within Psest_3006at 3195.420 kb on + strand, within Psest_3006at 3195.421 kb on - strand, within Psest_3006at 3195.421 kb on - strand, within Psest_3006at 3195.421 kb on - strand, within Psest_3006at 3195.421 kb on - strand, within Psest_3006at 3195.425 kb on - strand, within Psest_3006at 3195.425 kb on - strand, within Psest_3006at 3196.027 kb on + strand, within Psest_3007at 3196.027 kb on + strand, within Psest_3007at 3196.027 kb on + strand, within Psest_3007at 3196.027 kb on + strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.218 kb on + strandat 3196.218 kb on + strandat 3196.302 kb on + strand, within Psest_3008at 3196.302 kb on + strand, within Psest_3008at 3196.303 kb on - strand, within Psest_3008at 3196.303 kb on - strand, within Psest_3008at 3196.754 kb on + strand, within Psest_3008at 3196.754 kb on + strand, within Psest_3008at 3196.755 kb on - strand, within Psest_3008at 3197.260 kb on - strand, within Psest_3008at 3197.260 kb on - strand, within Psest_3008at 3198.276 kb on - strand, within Psest_3009at 3198.467 kb on + strandat 3198.518 kb on + strandat 3198.561 kb on + strandat 3198.561 kb on + strandat 3198.562 kb on - strandat 3198.562 kb on - strandat 3198.562 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Def sodium fluoride 25 mM
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3,195,420 + Psest_3006 0.83 +1.9
3,195,420 + Psest_3006 0.83 -1.4
3,195,420 + Psest_3006 0.83 +0.9
3,195,421 - Psest_3006 0.83 +0.3
3,195,421 - Psest_3006 0.83 +0.3
3,195,421 - Psest_3006 0.83 -0.7
3,195,421 - Psest_3006 0.83 +1.5
3,195,425 - Psest_3006 0.84 +0.3
3,195,425 - Psest_3006 0.84 +0.3
3,196,027 + Psest_3007 0.83 +1.5
3,196,027 + Psest_3007 0.83 -0.5
3,196,027 + Psest_3007 0.83 +0.9
3,196,027 + Psest_3007 0.83 +1.0
3,196,028 - Psest_3007 0.83 +0.2
3,196,028 - Psest_3007 0.83 +0.6
3,196,028 - Psest_3007 0.83 -0.7
3,196,028 - Psest_3007 0.83 +1.6
3,196,028 - Psest_3007 0.83 +0.1
3,196,028 - Psest_3007 0.83 +0.9
3,196,028 - Psest_3007 0.83 +0.9
3,196,028 - Psest_3007 0.83 +0.9
3,196,028 - Psest_3007 0.83 +0.6
3,196,028 - Psest_3007 0.83 -0.9
3,196,028 - Psest_3007 0.83 -1.4
3,196,028 - Psest_3007 0.83 +0.3
3,196,028 - Psest_3007 0.83 -2.1
3,196,028 - Psest_3007 0.83 +0.4
3,196,218 + +0.1
3,196,218 + -0.7
3,196,302 + Psest_3008 0.13 +0.9
3,196,302 + Psest_3008 0.13 +1.2
3,196,303 - Psest_3008 0.13 -1.7
3,196,303 - Psest_3008 0.13 -0.9
3,196,754 + Psest_3008 0.44 +1.3
3,196,754 + Psest_3008 0.44 -0.1
3,196,755 - Psest_3008 0.44 +1.5
3,197,260 - Psest_3008 0.78 +1.3
3,197,260 - Psest_3008 0.78 +0.7
3,198,276 - Psest_3009 0.71 +0.4
3,198,467 + +1.9
3,198,518 + +2.5
3,198,561 + -0.1
3,198,561 + +0.9
3,198,562 - -1.3
3,198,562 - +0.4
3,198,562 - -0.5

Or see this region's nucleotide sequence