Strain Fitness in Shewanella oneidensis MR-1 around SO2501

Experiment: L-Threonine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2500 and tRNA-Tyr-4 are separated by 125 nucleotidestRNA-Tyr-4 and tRNA-Tyr-3 are separated by 39 nucleotidestRNA-Tyr-3 and tRNA-Tyr-2 are separated by 117 nucleotidestRNA-Tyr-2 and SO2501 are separated by 206 nucleotidesSO2501 and exsB are separated by 188 nucleotidesexsB and SO2504 overlap by 4 nucleotides SO2500: SO2500 - conserved hypothetical protein (NCBI ptt file), at 2,625,874 to 2,626,362 SO2500 SO_t045: tRNA-Tyr-4 - tRNA-Tyr (RefSeq), at 2,626,488 to 2,626,572 tRNA-Tyr-4 SO_t046: tRNA-Tyr-3 - tRNA-Tyr (RefSeq), at 2,626,612 to 2,626,696 tRNA-Tyr-3 SO_t047: tRNA-Tyr-2 - tRNA-Tyr (RefSeq), at 2,626,814 to 2,626,898 tRNA-Tyr-2 SO2501: SO2501 - radical activating enzyme (NCBI ptt file), at 2,627,105 to 2,627,773 SO2501 SO2503: exsB - exsB protein (NCBI ptt file), at 2,627,962 to 2,628,678 exsB SO2504: SO2504 - conserved hypothetical protein (NCBI ptt file), at 2,628,675 to 2,629,289 SO2504 Position (kb) 2627 2628Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2627.101 kb on - strandat 2627.134 kb on + strandat 2627.210 kb on - strand, within SO2501at 2627.217 kb on - strand, within SO2501at 2627.315 kb on + strand, within SO2501at 2627.315 kb on + strand, within SO2501at 2627.315 kb on + strand, within SO2501at 2627.323 kb on - strand, within SO2501at 2627.450 kb on + strand, within SO2501at 2627.520 kb on + strand, within SO2501at 2627.520 kb on + strand, within SO2501at 2627.528 kb on - strand, within SO2501at 2627.530 kb on - strand, within SO2501at 2627.590 kb on + strand, within SO2501at 2627.598 kb on - strand, within SO2501at 2627.646 kb on - strand, within SO2501at 2627.674 kb on + strand, within SO2501at 2627.919 kb on - strandat 2628.074 kb on + strand, within exsBat 2628.082 kb on - strand, within exsBat 2628.185 kb on + strand, within exsBat 2628.197 kb on + strand, within exsBat 2628.197 kb on + strand, within exsBat 2628.251 kb on - strand, within exsBat 2628.252 kb on - strand, within exsBat 2628.294 kb on + strand, within exsBat 2628.474 kb on - strand, within exsBat 2628.474 kb on - strand, within exsBat 2628.523 kb on + strand, within exsBat 2628.523 kb on + strand, within exsBat 2628.531 kb on - strand, within exsBat 2628.623 kb on - strandat 2628.673 kb on + strandat 2628.737 kb on - strand, within SO2504

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Threonine (N)
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2,627,101 - +1.3
2,627,134 + +1.2
2,627,210 - SO2501 0.16 +0.7
2,627,217 - SO2501 0.17 +0.4
2,627,315 + SO2501 0.31 +0.8
2,627,315 + SO2501 0.31 +1.3
2,627,315 + SO2501 0.31 +1.2
2,627,323 - SO2501 0.33 +0.8
2,627,450 + SO2501 0.52 -1.7
2,627,520 + SO2501 0.62 +0.1
2,627,520 + SO2501 0.62 +1.2
2,627,528 - SO2501 0.63 -2.2
2,627,530 - SO2501 0.64 -0.0
2,627,590 + SO2501 0.72 -0.5
2,627,598 - SO2501 0.74 -0.3
2,627,646 - SO2501 0.81 +0.4
2,627,674 + SO2501 0.85 +0.6
2,627,919 - +0.2
2,628,074 + exsB SO2503 0.16 +1.5
2,628,082 - exsB SO2503 0.17 +3.2
2,628,185 + exsB SO2503 0.31 +0.9
2,628,197 + exsB SO2503 0.33 +1.3
2,628,197 + exsB SO2503 0.33 +1.2
2,628,251 - exsB SO2503 0.40 +1.5
2,628,252 - exsB SO2503 0.40 +1.7
2,628,294 + exsB SO2503 0.46 -0.6
2,628,474 - exsB SO2503 0.71 +2.5
2,628,474 - exsB SO2503 0.71 +2.5
2,628,523 + exsB SO2503 0.78 +0.6
2,628,523 + exsB SO2503 0.78 +1.2
2,628,531 - exsB SO2503 0.79 +2.5
2,628,623 - +3.4
2,628,673 + +0.7
2,628,737 - SO2504 0.10 +2.1

Or see this region's nucleotide sequence