Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2500 and tRNA-Tyr-4 are separated by 125 nucleotides tRNA-Tyr-4 and tRNA-Tyr-3 are separated by 39 nucleotides tRNA-Tyr-3 and tRNA-Tyr-2 are separated by 117 nucleotides tRNA-Tyr-2 and SO2501 are separated by 206 nucleotides SO2501 and exsB are separated by 188 nucleotides exsB and SO2504 overlap by 4 nucleotides
SO2500: SO2500 - conserved hypothetical protein (NCBI ptt file), at 2,625,874 to 2,626,362
SO2500
SO_t045: tRNA-Tyr-4 - tRNA-Tyr (RefSeq), at 2,626,488 to 2,626,572
tRNA-Tyr-4
SO_t046: tRNA-Tyr-3 - tRNA-Tyr (RefSeq), at 2,626,612 to 2,626,696
tRNA-Tyr-3
SO_t047: tRNA-Tyr-2 - tRNA-Tyr (RefSeq), at 2,626,814 to 2,626,898
tRNA-Tyr-2
SO2501: SO2501 - radical activating enzyme (NCBI ptt file), at 2,627,105 to 2,627,773
SO2501
SO2503: exsB - exsB protein (NCBI ptt file), at 2,627,962 to 2,628,678
exsB
SO2504: SO2504 - conserved hypothetical protein (NCBI ptt file), at 2,628,675 to 2,629,289
SO2504
Position (kb)
2627
2628 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2627.101 kb on - strand at 2627.134 kb on + strand at 2627.210 kb on - strand, within SO2501 at 2627.217 kb on - strand, within SO2501 at 2627.315 kb on + strand, within SO2501 at 2627.315 kb on + strand, within SO2501 at 2627.315 kb on + strand, within SO2501 at 2627.323 kb on - strand, within SO2501 at 2627.450 kb on + strand, within SO2501 at 2627.520 kb on + strand, within SO2501 at 2627.520 kb on + strand, within SO2501 at 2627.528 kb on - strand, within SO2501 at 2627.530 kb on - strand, within SO2501 at 2627.590 kb on + strand, within SO2501 at 2627.598 kb on - strand, within SO2501 at 2627.646 kb on - strand, within SO2501 at 2627.674 kb on + strand, within SO2501 at 2627.919 kb on - strand at 2628.074 kb on + strand, within exsB at 2628.082 kb on - strand, within exsB at 2628.185 kb on + strand, within exsB at 2628.197 kb on + strand, within exsB at 2628.197 kb on + strand, within exsB at 2628.251 kb on - strand, within exsB at 2628.252 kb on - strand, within exsB at 2628.294 kb on + strand, within exsB at 2628.474 kb on - strand, within exsB at 2628.474 kb on - strand, within exsB at 2628.523 kb on + strand, within exsB at 2628.523 kb on + strand, within exsB at 2628.531 kb on - strand, within exsB at 2628.623 kb on - strand at 2628.673 kb on + strand at 2628.737 kb on - strand, within SO2504
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 2,627,101 - +1.3 2,627,134 + +1.2 2,627,210 - SO2501 0.16 +0.7 2,627,217 - SO2501 0.17 +0.4 2,627,315 + SO2501 0.31 +0.8 2,627,315 + SO2501 0.31 +1.3 2,627,315 + SO2501 0.31 +1.2 2,627,323 - SO2501 0.33 +0.8 2,627,450 + SO2501 0.52 -1.7 2,627,520 + SO2501 0.62 +0.1 2,627,520 + SO2501 0.62 +1.2 2,627,528 - SO2501 0.63 -2.2 2,627,530 - SO2501 0.64 -0.0 2,627,590 + SO2501 0.72 -0.5 2,627,598 - SO2501 0.74 -0.3 2,627,646 - SO2501 0.81 +0.4 2,627,674 + SO2501 0.85 +0.6 2,627,919 - +0.2 2,628,074 + exsB SO2503 0.16 +1.5 2,628,082 - exsB SO2503 0.17 +3.2 2,628,185 + exsB SO2503 0.31 +0.9 2,628,197 + exsB SO2503 0.33 +1.3 2,628,197 + exsB SO2503 0.33 +1.2 2,628,251 - exsB SO2503 0.40 +1.5 2,628,252 - exsB SO2503 0.40 +1.7 2,628,294 + exsB SO2503 0.46 -0.6 2,628,474 - exsB SO2503 0.71 +2.5 2,628,474 - exsB SO2503 0.71 +2.5 2,628,523 + exsB SO2503 0.78 +0.6 2,628,523 + exsB SO2503 0.78 +1.2 2,628,531 - exsB SO2503 0.79 +2.5 2,628,623 - +3.4 2,628,673 + +0.7 2,628,737 - SO2504 0.10 +2.1
Or see this region's nucleotide sequence