Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tyrS and SO1317 are separated by 438 nucleotides SO1317 and SO1318 are separated by 123 nucleotides SO1318 and SO1319 are separated by 1 nucleotides SO1319 and SO1320 overlap by 4 nucleotides
SO1315: tyrS - tyrosyl-tRNA synthetase (NCBI ptt file), at 1,371,199 to 1,372,395
tyrS
SO1317: SO1317 - hypothetical protein (NCBI ptt file), at 1,372,834 to 1,373,262
SO1317
SO1318: SO1318 - hypothetical protein (NCBI ptt file), at 1,373,386 to 1,373,877
SO1318
SO1319: SO1319 - putative transporter, required for glycine utilization (from data) , at 1,373,879 to 1,374,505
SO1319
SO1320: SO1320 - hypothetical protein (NCBI ptt file), at 1,374,502 to 1,374,891
SO1320
Position (kb)
1373
1374 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1372.444 kb on - strand at 1372.447 kb on + strand at 1372.849 kb on + strand at 1372.933 kb on - strand, within SO1317 at 1373.045 kb on - strand, within SO1317 at 1373.095 kb on - strand, within SO1317 at 1373.214 kb on + strand, within SO1317 at 1373.219 kb on + strand, within SO1317 at 1373.540 kb on + strand, within SO1318 at 1373.548 kb on - strand, within SO1318 at 1373.568 kb on + strand, within SO1318 at 1373.576 kb on - strand, within SO1318 at 1373.576 kb on - strand, within SO1318 at 1373.576 kb on - strand, within SO1318 at 1373.585 kb on + strand, within SO1318 at 1373.633 kb on - strand, within SO1318 at 1373.844 kb on + strand at 1373.968 kb on - strand, within SO1319 at 1373.968 kb on - strand, within SO1319 at 1374.010 kb on - strand, within SO1319 at 1374.019 kb on + strand, within SO1319 at 1374.023 kb on - strand, within SO1319 at 1374.023 kb on - strand, within SO1319 at 1374.027 kb on - strand, within SO1319 at 1374.084 kb on + strand, within SO1319 at 1374.202 kb on + strand, within SO1319 at 1374.202 kb on + strand, within SO1319 at 1374.202 kb on + strand, within SO1319 at 1374.205 kb on - strand, within SO1319 at 1374.286 kb on - strand, within SO1319 at 1374.376 kb on - strand, within SO1319 at 1374.381 kb on + strand, within SO1319 at 1374.487 kb on + strand at 1374.658 kb on + strand, within SO1320 at 1374.669 kb on + strand, within SO1320 at 1374.669 kb on + strand, within SO1320 at 1374.669 kb on + strand, within SO1320 at 1374.677 kb on - strand, within SO1320 at 1374.781 kb on + strand, within SO1320
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 1,372,444 - -0.1 1,372,447 + -0.5 1,372,849 + +1.0 1,372,933 - SO1317 0.23 +0.2 1,373,045 - SO1317 0.49 -1.0 1,373,095 - SO1317 0.61 +2.0 1,373,214 + SO1317 0.89 -0.3 1,373,219 + SO1317 0.90 -1.6 1,373,540 + SO1318 0.31 -0.4 1,373,548 - SO1318 0.33 -0.7 1,373,568 + SO1318 0.37 +0.7 1,373,576 - SO1318 0.39 -1.1 1,373,576 - SO1318 0.39 -0.2 1,373,576 - SO1318 0.39 -0.2 1,373,585 + SO1318 0.40 -0.5 1,373,633 - SO1318 0.50 +0.3 1,373,844 + +0.7 1,373,968 - SO1319 0.14 -0.7 1,373,968 - SO1319 0.14 -2.4 1,374,010 - SO1319 0.21 +0.8 1,374,019 + SO1319 0.22 +0.4 1,374,023 - SO1319 0.23 -0.4 1,374,023 - SO1319 0.23 -2.9 1,374,027 - SO1319 0.24 -0.9 1,374,084 + SO1319 0.33 +0.5 1,374,202 + SO1319 0.52 -0.4 1,374,202 + SO1319 0.52 -0.3 1,374,202 + SO1319 0.52 -1.1 1,374,205 - SO1319 0.52 +1.2 1,374,286 - SO1319 0.65 -0.3 1,374,376 - SO1319 0.79 +0.4 1,374,381 + SO1319 0.80 -0.8 1,374,487 + -0.3 1,374,658 + SO1320 0.40 +0.6 1,374,669 + SO1320 0.43 +1.0 1,374,669 + SO1320 0.43 +1.0 1,374,669 + SO1320 0.43 +1.5 1,374,677 - SO1320 0.45 +0.1 1,374,781 + SO1320 0.72 +1.1
Or see this region's nucleotide sequence