Strain Fitness in Shewanella oneidensis MR-1 around SO1221

Experiment: L-Threonine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdeoB and deoD-2 are separated by 91 nucleotidesdeoD-2 and SO1222 are separated by 167 nucleotides SO1219: deoB - Phosphopentomutase (EC 5.4.2.7) (from data), at 1,266,092 to 1,267,306 deoB SO1221: deoD-2 - purine nucleoside phosphorylase (NCBI ptt file), at 1,267,398 to 1,268,108 deoD-2 SO1222: SO1222 - hypothetical protein (NCBI ptt file), at 1,268,276 to 1,269,499 SO1222 Position (kb) 1267 1268 1269Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5at 1266.485 kb on + strand, within deoBat 1266.495 kb on + strand, within deoBat 1266.554 kb on + strand, within deoBat 1266.706 kb on - strand, within deoBat 1266.876 kb on - strand, within deoBat 1266.876 kb on - strand, within deoBat 1266.912 kb on + strand, within deoBat 1267.025 kb on - strand, within deoBat 1267.093 kb on - strand, within deoBat 1267.139 kb on + strand, within deoBat 1267.149 kb on + strand, within deoBat 1267.157 kb on - strand, within deoBat 1267.157 kb on - strand, within deoBat 1267.186 kb on - strandat 1267.346 kb on - strandat 1267.346 kb on - strandat 1267.346 kb on - strandat 1267.346 kb on - strandat 1267.412 kb on + strandat 1267.479 kb on - strand, within deoD-2at 1267.573 kb on - strand, within deoD-2at 1267.687 kb on - strand, within deoD-2at 1267.699 kb on - strand, within deoD-2at 1267.724 kb on + strand, within deoD-2at 1267.732 kb on - strand, within deoD-2at 1267.807 kb on - strand, within deoD-2at 1267.965 kb on + strand, within deoD-2at 1268.038 kb on + strandat 1268.105 kb on + strandat 1268.113 kb on - strandat 1268.116 kb on + strandat 1268.116 kb on + strandat 1268.116 kb on + strandat 1268.116 kb on + strandat 1268.116 kb on + strandat 1268.124 kb on - strandat 1268.124 kb on - strandat 1268.124 kb on - strandat 1268.124 kb on - strandat 1268.124 kb on - strandat 1268.124 kb on - strandat 1268.124 kb on - strandat 1268.124 kb on - strandat 1268.124 kb on - strandat 1268.124 kb on - strandat 1268.124 kb on - strandat 1268.124 kb on - strandat 1268.351 kb on - strandat 1268.454 kb on + strand, within SO1222at 1268.467 kb on - strand, within SO1222at 1268.501 kb on + strand, within SO1222at 1268.503 kb on + strand, within SO1222at 1268.509 kb on - strand, within SO1222at 1268.600 kb on - strand, within SO1222at 1268.605 kb on + strand, within SO1222at 1268.701 kb on - strand, within SO1222at 1268.812 kb on + strand, within SO1222at 1268.835 kb on + strand, within SO1222at 1268.843 kb on - strand, within SO1222at 1268.998 kb on - strand, within SO1222

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Threonine (N)
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1,266,485 + deoB SO1219 0.32 -0.7
1,266,495 + deoB SO1219 0.33 -0.8
1,266,554 + deoB SO1219 0.38 -0.4
1,266,706 - deoB SO1219 0.51 -0.4
1,266,876 - deoB SO1219 0.65 -0.9
1,266,876 - deoB SO1219 0.65 -1.3
1,266,912 + deoB SO1219 0.67 -1.4
1,267,025 - deoB SO1219 0.77 -2.2
1,267,093 - deoB SO1219 0.82 -0.4
1,267,139 + deoB SO1219 0.86 -0.7
1,267,149 + deoB SO1219 0.87 +0.6
1,267,157 - deoB SO1219 0.88 -1.3
1,267,157 - deoB SO1219 0.88 -2.2
1,267,186 - -4.5
1,267,346 - -3.0
1,267,346 - -1.1
1,267,346 - -2.5
1,267,346 - -0.2
1,267,412 + -2.3
1,267,479 - deoD-2 SO1221 0.11 -2.3
1,267,573 - deoD-2 SO1221 0.25 -1.7
1,267,687 - deoD-2 SO1221 0.41 -2.6
1,267,699 - deoD-2 SO1221 0.42 -3.0
1,267,724 + deoD-2 SO1221 0.46 -2.7
1,267,732 - deoD-2 SO1221 0.47 -2.6
1,267,807 - deoD-2 SO1221 0.58 -2.6
1,267,965 + deoD-2 SO1221 0.80 -1.3
1,268,038 + -2.6
1,268,105 + +0.0
1,268,113 - -0.3
1,268,116 + -0.2
1,268,116 + -2.6
1,268,116 + -0.7
1,268,116 + +0.0
1,268,116 + +0.9
1,268,124 - +0.8
1,268,124 - +1.4
1,268,124 - -1.0
1,268,124 - +0.7
1,268,124 - +0.3
1,268,124 - +1.4
1,268,124 - +0.9
1,268,124 - +0.8
1,268,124 - +3.9
1,268,124 - -0.7
1,268,124 - +0.2
1,268,124 - -1.3
1,268,351 - +0.2
1,268,454 + SO1222 0.15 +0.4
1,268,467 - SO1222 0.16 +0.7
1,268,501 + SO1222 0.18 +0.7
1,268,503 + SO1222 0.19 +0.8
1,268,509 - SO1222 0.19 +0.4
1,268,600 - SO1222 0.26 +0.4
1,268,605 + SO1222 0.27 -1.0
1,268,701 - SO1222 0.35 -2.8
1,268,812 + SO1222 0.44 +0.9
1,268,835 + SO1222 0.46 -0.5
1,268,843 - SO1222 0.46 +0.2
1,268,998 - SO1222 0.59 +4.9

Or see this region's nucleotide sequence