Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrA and slyD are separated by 641 nucleotides slyD and SO3417.1 overlap by 212 nucleotides SO3417.1 and SO3418 are separated by 173 nucleotides SO3418 and trpR are separated by 32 nucleotides
SO3415: thrA - aspartokinase I/homoserine dehydrogenase, threonine-sensitive (NCBI ptt file), at 3,555,207 to 3,557,675
thrA
SO3417: slyD - peptidyl-prolyl cis-trans isomerase SlyD (NCBI ptt file), at 3,558,317 to 3,558,985
slyD
SO3417.1: SO3417.1 - peptidyl-prolyl cis-trans isomerase SlyD (NCBI ptt file), at 3,558,774 to 3,559,004
SO3417.1
SO3418: SO3418 - hypothetical protein (NCBI ptt file), at 3,559,178 to 3,559,555
SO3418
SO3419: trpR - trp operon repressor (NCBI ptt file), at 3,559,588 to 3,559,863
trpR
Position (kb)
3558
3559 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3557.424 kb on + strand, within thrA at 3557.437 kb on - strand at 3557.547 kb on - strand at 3557.573 kb on - strand at 3557.682 kb on + strand at 3557.690 kb on - strand at 3557.711 kb on - strand at 3557.721 kb on - strand at 3557.766 kb on - strand at 3557.779 kb on - strand at 3557.842 kb on - strand at 3557.851 kb on + strand at 3557.859 kb on - strand at 3557.859 kb on - strand at 3558.135 kb on - strand at 3558.227 kb on - strand at 3558.306 kb on - strand at 3558.549 kb on - strand, within slyD at 3558.693 kb on - strand, within slyD at 3558.926 kb on + strand at 3558.934 kb on - strand at 3559.011 kb on + strand at 3559.013 kb on + strand at 3559.075 kb on + strand at 3559.083 kb on - strand at 3559.172 kb on + strand at 3559.180 kb on - strand at 3559.180 kb on - strand at 3559.182 kb on - strand at 3559.196 kb on - strand at 3559.233 kb on + strand, within SO3418 at 3559.233 kb on + strand, within SO3418 at 3559.239 kb on + strand, within SO3418 at 3559.239 kb on + strand, within SO3418 at 3559.240 kb on - strand, within SO3418 at 3559.241 kb on - strand, within SO3418 at 3559.247 kb on - strand, within SO3418 at 3559.247 kb on - strand, within SO3418 at 3559.247 kb on - strand, within SO3418 at 3559.265 kb on - strand, within SO3418 at 3559.367 kb on - strand, within SO3418 at 3559.418 kb on + strand, within SO3418 at 3559.426 kb on - strand, within SO3418 at 3559.480 kb on - strand, within SO3418 at 3559.482 kb on + strand, within SO3418 at 3559.482 kb on + strand, within SO3418 at 3559.482 kb on + strand, within SO3418 at 3559.482 kb on + strand, within SO3418 at 3559.490 kb on - strand, within SO3418 at 3559.509 kb on + strand, within SO3418 at 3559.570 kb on + strand at 3559.622 kb on + strand, within trpR at 3559.652 kb on - strand, within trpR at 3559.670 kb on + strand, within trpR at 3559.675 kb on + strand, within trpR at 3559.681 kb on + strand, within trpR at 3559.765 kb on + strand, within trpR at 3559.765 kb on - strand, within trpR at 3559.767 kb on + strand, within trpR at 3559.773 kb on - strand, within trpR at 3559.775 kb on - strand, within trpR at 3559.775 kb on - strand, within trpR at 3559.803 kb on + strand, within trpR at 3559.854 kb on - strand at 3559.910 kb on + strand at 3559.910 kb on + strand at 3559.910 kb on + strand at 3559.910 kb on - strand at 3559.918 kb on - strand at 3559.918 kb on - strand at 3559.933 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 3,557,424 + thrA SO3415 0.90 -3.9 3,557,437 - -3.8 3,557,547 - -0.9 3,557,573 - -2.7 3,557,682 + -2.6 3,557,690 - -0.3 3,557,711 - -1.2 3,557,721 - -0.8 3,557,766 - -4.4 3,557,779 - -4.7 3,557,842 - -1.3 3,557,851 + -1.4 3,557,859 - -3.3 3,557,859 - -1.8 3,558,135 - +0.7 3,558,227 - -0.7 3,558,306 - +2.1 3,558,549 - slyD SO3417 0.35 +1.8 3,558,693 - slyD SO3417 0.56 +1.5 3,558,926 + +1.2 3,558,934 - +1.0 3,559,011 + +1.6 3,559,013 + +0.5 3,559,075 + +1.6 3,559,083 - +2.4 3,559,172 + -0.0 3,559,180 - -0.5 3,559,180 - -0.6 3,559,182 - +0.2 3,559,196 - +0.1 3,559,233 + SO3418 0.15 -1.0 3,559,233 + SO3418 0.15 -2.1 3,559,239 + SO3418 0.16 -0.6 3,559,239 + SO3418 0.16 +0.6 3,559,240 - SO3418 0.16 +0.5 3,559,241 - SO3418 0.17 -0.4 3,559,247 - SO3418 0.18 +0.1 3,559,247 - SO3418 0.18 +0.3 3,559,247 - SO3418 0.18 -0.9 3,559,265 - SO3418 0.23 -0.9 3,559,367 - SO3418 0.50 -0.8 3,559,418 + SO3418 0.63 +0.3 3,559,426 - SO3418 0.66 +2.1 3,559,480 - SO3418 0.80 +0.1 3,559,482 + SO3418 0.80 -0.1 3,559,482 + SO3418 0.80 +0.6 3,559,482 + SO3418 0.80 +0.2 3,559,482 + SO3418 0.80 -0.4 3,559,490 - SO3418 0.83 +1.0 3,559,509 + SO3418 0.88 +0.1 3,559,570 + +0.2 3,559,622 + trpR SO3419 0.12 +3.9 3,559,652 - trpR SO3419 0.23 +3.3 3,559,670 + trpR SO3419 0.30 +2.2 3,559,675 + trpR SO3419 0.32 +1.7 3,559,681 + trpR SO3419 0.34 +0.9 3,559,765 + trpR SO3419 0.64 +2.3 3,559,765 - trpR SO3419 0.64 +1.2 3,559,767 + trpR SO3419 0.65 +1.3 3,559,773 - trpR SO3419 0.67 +0.9 3,559,775 - trpR SO3419 0.68 +1.0 3,559,775 - trpR SO3419 0.68 +0.8 3,559,803 + trpR SO3419 0.78 +2.1 3,559,854 - +0.7 3,559,910 + -0.6 3,559,910 + +0.0 3,559,910 + +2.1 3,559,910 - +1.1 3,559,918 - +2.1 3,559,918 - +0.3 3,559,933 - +1.4
Or see this region's nucleotide sequence