Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4321 and SO4322 are separated by 18 nucleotides SO4322 and SO4323 are separated by 8 nucleotides SO4323 and tRNA-Pro-3 are separated by 184 nucleotides tRNA-Pro-3 and tRNA-Pro-2 are separated by 53 nucleotides tRNA-Pro-2 and tRNA-His-2 are separated by 15 nucleotides tRNA-His-2 and tRNA-Arg-2 are separated by 52 nucleotides tRNA-Arg-2 and SO4324 are separated by 206 nucleotides
SO4321: SO4321 - OmpA family protein (NCBI ptt file), at 4,508,558 to 4,509,163
SO4321
SO4322: SO4322 - conserved hypothetical protein (NCBI ptt file), at 4,509,182 to 4,509,889
SO4322
SO4323: SO4323 - GGDEF domain protein (NCBI ptt file), at 4,509,898 to 4,511,817
SO4323
SO_t097: tRNA-Pro-3 - tRNA-Pro (RefSeq), at 4,512,002 to 4,512,078
tRNA-Pro-3
SO_t098: tRNA-Pro-2 - tRNA-Pro (RefSeq), at 4,512,132 to 4,512,208
tRNA-Pro-2
SO_t099: tRNA-His-2 - tRNA-His (RefSeq), at 4,512,224 to 4,512,299
tRNA-His-2
SO_t100: tRNA-Arg-2 - tRNA-Arg (RefSeq), at 4,512,352 to 4,512,428
tRNA-Arg-2
SO4324: SO4324 - GGDEF domain protein (NCBI ptt file), at 4,512,635 to 4,515,265
SO4324
Position (kb)
4509
4510
4511
4512 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4508.923 kb on - strand, within SO4321 at 4508.990 kb on + strand, within SO4321 at 4509.014 kb on + strand, within SO4321 at 4509.020 kb on + strand, within SO4321 at 4509.022 kb on - strand, within SO4321 at 4509.034 kb on - strand, within SO4321 at 4509.044 kb on - strand, within SO4321 at 4509.078 kb on + strand, within SO4321 at 4509.079 kb on + strand, within SO4321 at 4509.087 kb on - strand, within SO4321 at 4509.097 kb on + strand, within SO4321 at 4509.098 kb on - strand, within SO4321 at 4509.165 kb on + strand at 4509.262 kb on - strand, within SO4322 at 4509.264 kb on + strand, within SO4322 at 4509.344 kb on + strand, within SO4322 at 4509.363 kb on + strand, within SO4322 at 4509.481 kb on + strand, within SO4322 at 4509.489 kb on - strand, within SO4322 at 4509.489 kb on - strand, within SO4322 at 4509.558 kb on - strand, within SO4322 at 4509.562 kb on + strand, within SO4322 at 4509.637 kb on + strand, within SO4322 at 4509.766 kb on - strand, within SO4322 at 4509.836 kb on + strand at 4509.838 kb on - strand at 4509.843 kb on + strand at 4509.843 kb on + strand at 4509.843 kb on + strand at 4509.843 kb on + strand at 4509.844 kb on - strand at 4509.866 kb on - strand at 4509.866 kb on - strand at 4509.866 kb on - strand at 4509.896 kb on + strand at 4509.904 kb on - strand at 4509.939 kb on + strand at 4509.958 kb on + strand at 4509.959 kb on + strand at 4509.959 kb on + strand at 4509.966 kb on - strand at 4509.993 kb on - strand at 4510.018 kb on + strand at 4510.026 kb on - strand at 4510.026 kb on - strand at 4510.054 kb on - strand at 4510.098 kb on - strand, within SO4323 at 4510.265 kb on + strand, within SO4323 at 4510.274 kb on + strand, within SO4323 at 4510.502 kb on - strand, within SO4323 at 4510.761 kb on - strand, within SO4323 at 4510.886 kb on - strand, within SO4323 at 4510.912 kb on - strand, within SO4323 at 4510.932 kb on + strand, within SO4323 at 4510.973 kb on + strand, within SO4323 at 4510.975 kb on + strand, within SO4323 at 4511.131 kb on - strand, within SO4323 at 4511.242 kb on - strand, within SO4323 at 4511.267 kb on - strand, within SO4323 at 4511.278 kb on - strand, within SO4323 at 4511.537 kb on - strand, within SO4323 at 4511.537 kb on - strand, within SO4323 at 4511.597 kb on + strand, within SO4323 at 4511.597 kb on + strand, within SO4323 at 4511.602 kb on + strand, within SO4323 at 4511.612 kb on + strand, within SO4323 at 4511.644 kb on + strand at 4511.678 kb on - strand at 4511.727 kb on - strand at 4511.771 kb on - strand at 4511.863 kb on - strand at 4511.910 kb on + strand at 4512.089 kb on + strand at 4512.146 kb on - strand, within tRNA-Pro-2 at 4512.606 kb on + strand at 4512.611 kb on + strand at 4512.619 kb on - strand at 4512.630 kb on + strand at 4512.790 kb on + strand at 4512.790 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,508,923 - SO4321 0.60 -0.0 4,508,990 + SO4321 0.71 -0.5 4,509,014 + SO4321 0.75 -0.5 4,509,020 + SO4321 0.76 +0.4 4,509,022 - SO4321 0.77 -0.8 4,509,034 - SO4321 0.79 +0.8 4,509,044 - SO4321 0.80 +1.1 4,509,078 + SO4321 0.86 -0.0 4,509,079 + SO4321 0.86 +0.7 4,509,087 - SO4321 0.87 -0.2 4,509,097 + SO4321 0.89 -0.1 4,509,098 - SO4321 0.89 -0.1 4,509,165 + +0.8 4,509,262 - SO4322 0.11 -0.2 4,509,264 + SO4322 0.12 -0.5 4,509,344 + SO4322 0.23 -0.5 4,509,363 + SO4322 0.26 -0.2 4,509,481 + SO4322 0.42 +0.4 4,509,489 - SO4322 0.43 -0.3 4,509,489 - SO4322 0.43 +0.7 4,509,558 - SO4322 0.53 -0.0 4,509,562 + SO4322 0.54 +0.8 4,509,637 + SO4322 0.64 -0.5 4,509,766 - SO4322 0.82 +0.3 4,509,836 + -0.5 4,509,838 - +0.0 4,509,843 + +2.0 4,509,843 + +0.6 4,509,843 + -0.0 4,509,843 + +0.2 4,509,844 - +0.1 4,509,866 - -0.4 4,509,866 - +0.3 4,509,866 - +1.0 4,509,896 + +0.7 4,509,904 - -1.2 4,509,939 + +0.2 4,509,958 + -0.6 4,509,959 + -0.2 4,509,959 + +0.2 4,509,966 - -0.3 4,509,993 - -0.2 4,510,018 + +0.1 4,510,026 - +0.6 4,510,026 - -0.4 4,510,054 - -0.0 4,510,098 - SO4323 0.10 +0.5 4,510,265 + SO4323 0.19 +0.6 4,510,274 + SO4323 0.20 +0.1 4,510,502 - SO4323 0.31 -0.7 4,510,761 - SO4323 0.45 +0.5 4,510,886 - SO4323 0.51 +0.3 4,510,912 - SO4323 0.53 +0.7 4,510,932 + SO4323 0.54 +0.3 4,510,973 + SO4323 0.56 +0.3 4,510,975 + SO4323 0.56 +0.1 4,511,131 - SO4323 0.64 +0.3 4,511,242 - SO4323 0.70 +0.2 4,511,267 - SO4323 0.71 -0.2 4,511,278 - SO4323 0.72 +0.4 4,511,537 - SO4323 0.85 -0.8 4,511,537 - SO4323 0.85 -1.8 4,511,597 + SO4323 0.88 +0.7 4,511,597 + SO4323 0.88 +0.6 4,511,602 + SO4323 0.89 +0.6 4,511,612 + SO4323 0.89 +1.3 4,511,644 + +0.6 4,511,678 - +0.5 4,511,727 - +0.4 4,511,771 - +0.0 4,511,863 - +0.3 4,511,910 + -0.4 4,512,089 + +0.8 4,512,146 - tRNA-Pro-2 SO_t098 0.18 -1.5 4,512,606 + -0.6 4,512,611 + +0.3 4,512,619 - +0.1 4,512,630 + -0.5 4,512,790 + -0.2 4,512,790 + -0.3
Or see this region's nucleotide sequence