Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mshA and mshB are separated by 85 nucleotides mshB and SO4107 are separated by 76 nucleotides SO4107 and mshG are separated by 26 nucleotides
SO4105: mshA - MSHA pilin protein MshA (NCBI ptt file), at 4,263,793 to 4,264,308
mshA
SO4106: mshB - MSHA pilin protein MshB (NCBI ptt file), at 4,264,394 to 4,265,011
mshB
SO4107: SO4107 - hypothetical protein (NCBI ptt file), at 4,265,088 to 4,265,582
SO4107
SO4108: mshG - MSHA biogenesis protein MshG (NCBI ptt file), at 4,265,609 to 4,266,829
mshG
Position (kb)
4265
4266 Strain fitness (log2 ratio)
-2
-1
0
1 at 4264.093 kb on + strand, within mshA at 4264.170 kb on + strand, within mshA at 4264.171 kb on + strand, within mshA at 4264.171 kb on + strand, within mshA at 4264.171 kb on + strand, within mshA at 4264.171 kb on + strand, within mshA at 4264.171 kb on + strand, within mshA at 4264.178 kb on - strand, within mshA at 4264.179 kb on - strand, within mshA at 4264.180 kb on + strand, within mshA at 4264.180 kb on + strand, within mshA at 4264.212 kb on - strand, within mshA at 4264.247 kb on - strand, within mshA at 4264.272 kb on - strand at 4264.284 kb on + strand at 4264.285 kb on + strand at 4264.292 kb on - strand at 4264.293 kb on - strand at 4264.312 kb on + strand at 4264.315 kb on - strand at 4264.416 kb on - strand at 4264.421 kb on + strand at 4264.421 kb on + strand at 4264.421 kb on + strand at 4264.421 kb on + strand at 4264.421 kb on + strand at 4264.429 kb on - strand at 4264.429 kb on - strand at 4264.429 kb on - strand at 4264.429 kb on - strand at 4264.429 kb on - strand at 4264.451 kb on - strand at 4264.458 kb on - strand, within mshB at 4264.481 kb on + strand, within mshB at 4264.481 kb on + strand, within mshB at 4264.481 kb on + strand, within mshB at 4264.488 kb on - strand, within mshB at 4264.491 kb on + strand, within mshB at 4264.496 kb on + strand, within mshB at 4264.504 kb on - strand, within mshB at 4264.562 kb on + strand, within mshB at 4264.562 kb on + strand, within mshB at 4264.568 kb on - strand, within mshB at 4264.570 kb on - strand, within mshB at 4264.597 kb on + strand, within mshB at 4264.623 kb on + strand, within mshB at 4264.623 kb on + strand, within mshB at 4264.623 kb on + strand, within mshB at 4264.626 kb on - strand, within mshB at 4264.628 kb on + strand, within mshB at 4264.631 kb on - strand, within mshB at 4264.631 kb on - strand, within mshB at 4264.765 kb on - strand, within mshB at 4264.825 kb on - strand, within mshB at 4264.889 kb on + strand, within mshB at 4264.930 kb on - strand, within mshB at 4265.170 kb on + strand, within SO4107 at 4265.170 kb on + strand, within SO4107 at 4265.170 kb on + strand, within SO4107 at 4265.178 kb on - strand, within SO4107 at 4265.281 kb on + strand, within SO4107 at 4265.387 kb on + strand, within SO4107 at 4265.395 kb on - strand, within SO4107 at 4265.395 kb on - strand, within SO4107 at 4265.414 kb on - strand, within SO4107 at 4265.459 kb on + strand, within SO4107 at 4265.619 kb on + strand at 4265.627 kb on - strand at 4265.633 kb on + strand at 4265.636 kb on - strand at 4265.745 kb on - strand, within mshG at 4265.831 kb on + strand, within mshG at 4265.931 kb on + strand, within mshG at 4265.931 kb on + strand, within mshG at 4265.965 kb on - strand, within mshG at 4266.037 kb on + strand, within mshG at 4266.191 kb on + strand, within mshG at 4266.212 kb on + strand, within mshG at 4266.225 kb on - strand, within mshG at 4266.315 kb on + strand, within mshG at 4266.390 kb on - strand, within mshG at 4266.390 kb on - strand, within mshG at 4266.416 kb on - strand, within mshG at 4266.416 kb on - strand, within mshG at 4266.416 kb on - strand, within mshG at 4266.462 kb on - strand, within mshG at 4266.503 kb on - strand, within mshG
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,264,093 + mshA SO4105 0.58 -0.3 4,264,170 + mshA SO4105 0.73 +0.2 4,264,171 + mshA SO4105 0.73 +0.0 4,264,171 + mshA SO4105 0.73 -0.1 4,264,171 + mshA SO4105 0.73 +0.0 4,264,171 + mshA SO4105 0.73 -0.1 4,264,171 + mshA SO4105 0.73 -0.7 4,264,178 - mshA SO4105 0.75 -0.3 4,264,179 - mshA SO4105 0.75 +1.3 4,264,180 + mshA SO4105 0.75 -0.1 4,264,180 + mshA SO4105 0.75 +0.6 4,264,212 - mshA SO4105 0.81 -0.2 4,264,247 - mshA SO4105 0.88 +0.2 4,264,272 - -0.0 4,264,284 + -0.4 4,264,285 + -1.3 4,264,292 - -0.2 4,264,293 - +0.2 4,264,312 + +0.0 4,264,315 - -0.2 4,264,416 - +0.3 4,264,421 + -0.6 4,264,421 + +0.2 4,264,421 + -1.4 4,264,421 + +0.7 4,264,421 + +0.5 4,264,429 - +0.0 4,264,429 - -0.4 4,264,429 - +0.4 4,264,429 - +0.6 4,264,429 - -0.4 4,264,451 - -0.6 4,264,458 - mshB SO4106 0.10 -0.0 4,264,481 + mshB SO4106 0.14 -0.2 4,264,481 + mshB SO4106 0.14 +0.3 4,264,481 + mshB SO4106 0.14 +0.3 4,264,488 - mshB SO4106 0.15 +0.3 4,264,491 + mshB SO4106 0.16 -0.5 4,264,496 + mshB SO4106 0.17 -0.3 4,264,504 - mshB SO4106 0.18 -0.2 4,264,562 + mshB SO4106 0.27 +0.5 4,264,562 + mshB SO4106 0.27 +0.3 4,264,568 - mshB SO4106 0.28 -0.1 4,264,570 - mshB SO4106 0.28 -0.3 4,264,597 + mshB SO4106 0.33 +0.7 4,264,623 + mshB SO4106 0.37 -1.9 4,264,623 + mshB SO4106 0.37 -0.1 4,264,623 + mshB SO4106 0.37 +0.6 4,264,626 - mshB SO4106 0.38 -1.0 4,264,628 + mshB SO4106 0.38 +1.0 4,264,631 - mshB SO4106 0.38 -0.6 4,264,631 - mshB SO4106 0.38 -0.2 4,264,765 - mshB SO4106 0.60 +0.6 4,264,825 - mshB SO4106 0.70 -0.1 4,264,889 + mshB SO4106 0.80 +0.4 4,264,930 - mshB SO4106 0.87 -0.1 4,265,170 + SO4107 0.17 +0.5 4,265,170 + SO4107 0.17 -0.3 4,265,170 + SO4107 0.17 +0.7 4,265,178 - SO4107 0.18 +0.1 4,265,281 + SO4107 0.39 +0.4 4,265,387 + SO4107 0.60 -0.3 4,265,395 - SO4107 0.62 +1.1 4,265,395 - SO4107 0.62 +0.3 4,265,414 - SO4107 0.66 +0.3 4,265,459 + SO4107 0.75 +0.2 4,265,619 + -0.2 4,265,627 - -0.2 4,265,633 + +0.3 4,265,636 - +0.7 4,265,745 - mshG SO4108 0.11 +0.2 4,265,831 + mshG SO4108 0.18 -0.3 4,265,931 + mshG SO4108 0.26 -0.1 4,265,931 + mshG SO4108 0.26 +0.9 4,265,965 - mshG SO4108 0.29 +1.1 4,266,037 + mshG SO4108 0.35 -0.2 4,266,191 + mshG SO4108 0.48 +0.1 4,266,212 + mshG SO4108 0.49 +0.3 4,266,225 - mshG SO4108 0.50 +0.4 4,266,315 + mshG SO4108 0.58 -0.1 4,266,390 - mshG SO4108 0.64 +0.2 4,266,390 - mshG SO4108 0.64 -0.3 4,266,416 - mshG SO4108 0.66 -0.6 4,266,416 - mshG SO4108 0.66 +0.5 4,266,416 - mshG SO4108 0.66 -0.3 4,266,462 - mshG SO4108 0.70 -0.5 4,266,503 - mshG SO4108 0.73 +0.3
Or see this region's nucleotide sequence