Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoN and yfiA-3 are separated by 28 nucleotides yfiA-3 and ptsN are separated by 2 nucleotides ptsN and SO3964 overlap by 4 nucleotides SO3964 and ptsO overlap by 14 nucleotides ptsO and mgtE-2 are separated by 196 nucleotides
SO3961: rpoN - RNA polymerase sigma-54 factor (NCBI ptt file), at 4,102,103 to 4,103,578
rpoN
SO3962: yfiA-3 - ribosomal subunit interface protein (NCBI ptt file), at 4,103,607 to 4,103,894
yfiA-3
SO3963: ptsN - nitrogen regulatory IIA protein (NCBI ptt file), at 4,103,897 to 4,104,340
ptsN
SO3964: SO3964 - conserved hypothetical protein (NCBI ptt file), at 4,104,337 to 4,105,191
SO3964
SO3965: ptsO - phosphocarrier protein NPR (NCBI ptt file), at 4,105,178 to 4,105,453
ptsO
SO3966: mgtE-2 - magnesium transporter (NCBI ptt file), at 4,105,650 to 4,107,014
mgtE-2
Position (kb)
4104
4105
4106 Strain fitness (log2 ratio)
-2
-1
0
1 at 4103.365 kb on + strand, within rpoN at 4103.365 kb on + strand, within rpoN at 4103.373 kb on - strand, within rpoN at 4103.373 kb on - strand, within rpoN at 4103.383 kb on - strand, within rpoN at 4103.389 kb on + strand, within rpoN at 4103.389 kb on + strand, within rpoN at 4103.397 kb on - strand, within rpoN at 4103.487 kb on - strand at 4103.535 kb on - strand at 4103.561 kb on - strand at 4103.563 kb on + strand at 4103.563 kb on + strand at 4103.642 kb on - strand, within yfiA-3 at 4103.657 kb on + strand, within yfiA-3 at 4103.705 kb on + strand, within yfiA-3 at 4103.823 kb on + strand, within yfiA-3 at 4103.895 kb on + strand at 4103.942 kb on + strand, within ptsN at 4103.942 kb on + strand, within ptsN at 4103.950 kb on - strand, within ptsN at 4103.993 kb on + strand, within ptsN at 4104.001 kb on - strand, within ptsN at 4104.065 kb on + strand, within ptsN at 4104.065 kb on + strand, within ptsN at 4104.108 kb on + strand, within ptsN at 4104.278 kb on + strand, within ptsN at 4104.386 kb on + strand at 4104.394 kb on - strand at 4104.394 kb on - strand at 4104.465 kb on + strand, within SO3964 at 4104.473 kb on - strand, within SO3964 at 4104.473 kb on - strand, within SO3964 at 4104.574 kb on - strand, within SO3964 at 4104.942 kb on - strand, within SO3964 at 4105.286 kb on - strand, within ptsO at 4105.421 kb on + strand, within ptsO at 4105.443 kb on - strand at 4105.654 kb on - strand at 4105.680 kb on - strand at 4105.906 kb on + strand, within mgtE-2 at 4105.914 kb on - strand, within mgtE-2 at 4106.006 kb on + strand, within mgtE-2 at 4106.048 kb on + strand, within mgtE-2 at 4106.056 kb on - strand, within mgtE-2 at 4106.082 kb on - strand, within mgtE-2 at 4106.165 kb on - strand, within mgtE-2
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,103,365 + rpoN SO3961 0.86 -0.9 4,103,365 + rpoN SO3961 0.86 -1.4 4,103,373 - rpoN SO3961 0.86 +0.5 4,103,373 - rpoN SO3961 0.86 -1.4 4,103,383 - rpoN SO3961 0.87 -1.0 4,103,389 + rpoN SO3961 0.87 -0.5 4,103,389 + rpoN SO3961 0.87 -1.2 4,103,397 - rpoN SO3961 0.88 +0.1 4,103,487 - -0.6 4,103,535 - -0.2 4,103,561 - -0.2 4,103,563 + -0.2 4,103,563 + +0.3 4,103,642 - yfiA-3 SO3962 0.12 -0.5 4,103,657 + yfiA-3 SO3962 0.17 +0.0 4,103,705 + yfiA-3 SO3962 0.34 -0.4 4,103,823 + yfiA-3 SO3962 0.75 -1.4 4,103,895 + +0.2 4,103,942 + ptsN SO3963 0.10 -2.0 4,103,942 + ptsN SO3963 0.10 -1.1 4,103,950 - ptsN SO3963 0.12 -2.2 4,103,993 + ptsN SO3963 0.22 -0.8 4,104,001 - ptsN SO3963 0.23 -0.8 4,104,065 + ptsN SO3963 0.38 -1.8 4,104,065 + ptsN SO3963 0.38 +0.3 4,104,108 + ptsN SO3963 0.48 -2.5 4,104,278 + ptsN SO3963 0.86 +0.2 4,104,386 + -0.2 4,104,394 - -0.1 4,104,394 - +0.3 4,104,465 + SO3964 0.15 -1.3 4,104,473 - SO3964 0.16 +1.3 4,104,473 - SO3964 0.16 -0.1 4,104,574 - SO3964 0.28 +0.6 4,104,942 - SO3964 0.71 +0.2 4,105,286 - ptsO SO3965 0.39 +0.8 4,105,421 + ptsO SO3965 0.88 -0.5 4,105,443 - +0.1 4,105,654 - +0.1 4,105,680 - -0.8 4,105,906 + mgtE-2 SO3966 0.19 +1.1 4,105,914 - mgtE-2 SO3966 0.19 +0.5 4,106,006 + mgtE-2 SO3966 0.26 +0.1 4,106,048 + mgtE-2 SO3966 0.29 -0.1 4,106,056 - mgtE-2 SO3966 0.30 +0.2 4,106,082 - mgtE-2 SO3966 0.32 -0.0 4,106,165 - mgtE-2 SO3966 0.38 +0.5
Or see this region's nucleotide sequence