Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliQ and fliP are separated by 17 nucleotides fliP and fliO overlap by 1 nucleotides fliO and fliN are separated by 34 nucleotides fliN and fliM are separated by 12 nucleotides
SO3217: fliQ - flagellar biosynthetic protein FliQ (NCBI ptt file), at 3,357,569 to 3,357,838
fliQ
SO3218: fliP - flagellar biosynthetic protein FliP (NCBI ptt file), at 3,357,856 to 3,358,599
fliP
SO3219: fliO - flagellar protein FliO (NCBI ptt file), at 3,358,599 to 3,359,006
fliO
SO3220: fliN - flagellar motor switch protein FliN (NCBI ptt file), at 3,359,041 to 3,359,415
fliN
SO3221: fliM - flagellar motor switch protein FliM (NCBI ptt file), at 3,359,428 to 3,360,459
fliM
Position (kb)
3358
3359
3360 Strain fitness (log2 ratio)
-1
0
1 at 3357.623 kb on + strand, within fliQ at 3357.623 kb on + strand, within fliQ at 3357.670 kb on + strand, within fliQ at 3357.697 kb on - strand, within fliQ at 3357.697 kb on - strand, within fliQ at 3357.744 kb on + strand, within fliQ at 3357.758 kb on + strand, within fliQ at 3357.826 kb on + strand at 3357.858 kb on + strand at 3357.861 kb on - strand at 3357.953 kb on - strand, within fliP at 3357.960 kb on + strand, within fliP at 3357.970 kb on - strand, within fliP at 3358.088 kb on - strand, within fliP at 3358.152 kb on + strand, within fliP at 3358.196 kb on + strand, within fliP at 3358.204 kb on - strand, within fliP at 3358.249 kb on - strand, within fliP at 3358.319 kb on - strand, within fliP at 3358.321 kb on + strand, within fliP at 3358.436 kb on - strand, within fliP at 3358.485 kb on - strand, within fliP at 3358.565 kb on + strand at 3358.594 kb on - strand at 3358.619 kb on + strand at 3358.757 kb on + strand, within fliO at 3358.757 kb on + strand, within fliO at 3358.861 kb on + strand, within fliO at 3358.962 kb on + strand, within fliO at 3359.028 kb on - strand at 3359.074 kb on + strand at 3359.082 kb on - strand, within fliN at 3359.157 kb on + strand, within fliN at 3359.178 kb on + strand, within fliN at 3359.180 kb on + strand, within fliN at 3359.299 kb on - strand, within fliN at 3359.300 kb on + strand, within fliN at 3359.327 kb on + strand, within fliN at 3359.336 kb on + strand, within fliN at 3359.401 kb on - strand at 3359.408 kb on + strand at 3359.518 kb on + strand at 3359.684 kb on - strand, within fliM at 3359.732 kb on - strand, within fliM at 3359.742 kb on - strand, within fliM at 3359.829 kb on - strand, within fliM at 3359.894 kb on + strand, within fliM at 3359.909 kb on + strand, within fliM at 3359.947 kb on + strand, within fliM at 3359.955 kb on - strand, within fliM at 3359.966 kb on + strand, within fliM at 3359.974 kb on - strand, within fliM at 3360.006 kb on - strand, within fliM
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,357,623 + fliQ SO3217 0.20 -0.7 3,357,623 + fliQ SO3217 0.20 +0.2 3,357,670 + fliQ SO3217 0.37 +0.7 3,357,697 - fliQ SO3217 0.47 +1.7 3,357,697 - fliQ SO3217 0.47 -0.6 3,357,744 + fliQ SO3217 0.65 +0.5 3,357,758 + fliQ SO3217 0.70 +0.7 3,357,826 + -0.7 3,357,858 + +1.5 3,357,861 - +0.2 3,357,953 - fliP SO3218 0.13 -0.7 3,357,960 + fliP SO3218 0.14 -0.0 3,357,970 - fliP SO3218 0.15 +0.1 3,358,088 - fliP SO3218 0.31 +0.8 3,358,152 + fliP SO3218 0.40 +0.0 3,358,196 + fliP SO3218 0.46 +0.2 3,358,204 - fliP SO3218 0.47 +0.0 3,358,249 - fliP SO3218 0.53 +0.5 3,358,319 - fliP SO3218 0.62 +1.1 3,358,321 + fliP SO3218 0.62 +0.0 3,358,436 - fliP SO3218 0.78 +0.6 3,358,485 - fliP SO3218 0.85 +0.6 3,358,565 + +0.5 3,358,594 - +0.9 3,358,619 + -0.3 3,358,757 + fliO SO3219 0.39 +0.7 3,358,757 + fliO SO3219 0.39 +0.4 3,358,861 + fliO SO3219 0.64 -0.2 3,358,962 + fliO SO3219 0.89 +0.3 3,359,028 - +0.0 3,359,074 + +0.5 3,359,082 - fliN SO3220 0.11 +0.1 3,359,157 + fliN SO3220 0.31 -1.5 3,359,178 + fliN SO3220 0.37 -0.2 3,359,180 + fliN SO3220 0.37 -0.2 3,359,299 - fliN SO3220 0.69 -0.5 3,359,300 + fliN SO3220 0.69 +0.6 3,359,327 + fliN SO3220 0.76 -0.7 3,359,336 + fliN SO3220 0.79 +0.9 3,359,401 - +0.2 3,359,408 + -0.9 3,359,518 + -0.9 3,359,684 - fliM SO3221 0.25 +0.7 3,359,732 - fliM SO3221 0.29 +0.0 3,359,742 - fliM SO3221 0.30 +0.0 3,359,829 - fliM SO3221 0.39 +0.1 3,359,894 + fliM SO3221 0.45 -0.7 3,359,909 + fliM SO3221 0.47 +0.2 3,359,947 + fliM SO3221 0.50 +1.1 3,359,955 - fliM SO3221 0.51 +0.3 3,359,966 + fliM SO3221 0.52 -0.7 3,359,974 - fliM SO3221 0.53 +0.9 3,360,006 - fliM SO3221 0.56 +0.1
Or see this region's nucleotide sequence