Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sapB and sapA overlap by 1 nucleotides sapA and pspF are separated by 117 nucleotides
SO1804: sapB - peptide ABC transporter, permease protein (NCBI ptt file), at 1,893,833 to 1,894,864
sapB
SO1805: sapA - peptide ABC transporter, periplasmic peptide-binding protein (NCBI ptt file), at 1,894,864 to 1,896,441
sapA
SO1806: pspF - psp operon transcriptional activator (NCBI ptt file), at 1,896,559 to 1,897,650
pspF
Position (kb)
1894
1895
1896
1897 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1894.134 kb on + strand, within sapB at 1894.137 kb on - strand, within sapB at 1894.212 kb on - strand, within sapB at 1894.710 kb on + strand, within sapB at 1894.747 kb on - strand, within sapB at 1894.753 kb on + strand, within sapB at 1894.761 kb on - strand at 1894.761 kb on - strand at 1894.778 kb on - strand at 1894.984 kb on - strand at 1894.991 kb on + strand at 1895.567 kb on - strand, within sapA at 1896.031 kb on - strand, within sapA at 1896.197 kb on - strand, within sapA at 1896.222 kb on - strand, within sapA at 1896.543 kb on + strand at 1896.603 kb on + strand at 1896.611 kb on - strand at 1896.740 kb on + strand, within pspF at 1896.748 kb on + strand, within pspF at 1896.748 kb on - strand, within pspF at 1896.765 kb on + strand, within pspF at 1896.817 kb on + strand, within pspF at 1896.903 kb on + strand, within pspF at 1896.908 kb on + strand, within pspF at 1896.967 kb on + strand, within pspF at 1896.989 kb on - strand, within pspF at 1897.037 kb on + strand, within pspF at 1897.052 kb on + strand, within pspF at 1897.060 kb on - strand, within pspF at 1897.060 kb on - strand, within pspF at 1897.081 kb on + strand, within pspF at 1897.165 kb on - strand, within pspF at 1897.353 kb on + strand, within pspF at 1897.371 kb on - strand, within pspF
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,894,134 + sapB SO1804 0.29 -2.4 1,894,137 - sapB SO1804 0.29 -1.0 1,894,212 - sapB SO1804 0.37 -1.2 1,894,710 + sapB SO1804 0.85 -1.3 1,894,747 - sapB SO1804 0.89 -1.3 1,894,753 + sapB SO1804 0.89 -2.7 1,894,761 - -0.9 1,894,761 - -1.0 1,894,778 - -2.6 1,894,984 - -0.7 1,894,991 + -0.0 1,895,567 - sapA SO1805 0.45 -0.4 1,896,031 - sapA SO1805 0.74 -3.0 1,896,197 - sapA SO1805 0.84 -1.4 1,896,222 - sapA SO1805 0.86 -1.0 1,896,543 + -1.1 1,896,603 + -0.3 1,896,611 - +0.5 1,896,740 + pspF SO1806 0.17 +0.2 1,896,748 + pspF SO1806 0.17 +0.2 1,896,748 - pspF SO1806 0.17 +0.4 1,896,765 + pspF SO1806 0.19 -0.1 1,896,817 + pspF SO1806 0.24 +1.0 1,896,903 + pspF SO1806 0.32 -1.2 1,896,908 + pspF SO1806 0.32 +0.3 1,896,967 + pspF SO1806 0.37 -0.3 1,896,989 - pspF SO1806 0.39 +0.9 1,897,037 + pspF SO1806 0.44 -0.2 1,897,052 + pspF SO1806 0.45 -2.3 1,897,060 - pspF SO1806 0.46 -0.0 1,897,060 - pspF SO1806 0.46 +0.2 1,897,081 + pspF SO1806 0.48 +0.8 1,897,165 - pspF SO1806 0.55 +0.6 1,897,353 + pspF SO1806 0.73 -1.2 1,897,371 - pspF SO1806 0.74 -0.5
Or see this region's nucleotide sequence