Experiment: L-Lysine 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoS and nlpD are separated by 81 nucleotides nlpD and pcm are separated by 57 nucleotides pcm and surE overlap by 4 nucleotides
SO3432: rpoS - RNA polymerase sigma-38 factor (NCBI ptt file), at 3,577,258 to 3,578,238
rpoS
SO3433: nlpD - lipoprotein NlpD (NCBI ptt file), at 3,578,320 to 3,579,216
nlpD
SO3434: pcm - protein-L-isoaspartate O-methyltransferase (NCBI ptt file), at 3,579,274 to 3,579,909
pcm
SO3435: surE - stationary-phase survival protein SurE (NCBI ptt file), at 3,579,906 to 3,580,652
surE
Position (kb)
3578
3579
3580 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3577.344 kb on - strand at 3577.448 kb on + strand, within rpoS at 3577.456 kb on - strand, within rpoS at 3577.495 kb on + strand, within rpoS at 3577.528 kb on - strand, within rpoS at 3577.552 kb on + strand, within rpoS at 3577.626 kb on + strand, within rpoS at 3577.634 kb on - strand, within rpoS at 3577.634 kb on - strand, within rpoS at 3577.639 kb on + strand, within rpoS at 3577.640 kb on - strand, within rpoS at 3577.721 kb on + strand, within rpoS at 3577.729 kb on - strand, within rpoS at 3577.765 kb on + strand, within rpoS at 3577.956 kb on + strand, within rpoS at 3578.074 kb on - strand, within rpoS at 3578.168 kb on + strand at 3578.338 kb on + strand at 3578.343 kb on - strand at 3578.353 kb on - strand at 3578.394 kb on + strand at 3578.402 kb on - strand at 3578.464 kb on + strand, within nlpD at 3578.464 kb on + strand, within nlpD at 3578.642 kb on - strand, within nlpD at 3578.685 kb on + strand, within nlpD at 3578.685 kb on + strand, within nlpD at 3578.685 kb on + strand, within nlpD at 3578.693 kb on - strand, within nlpD at 3578.693 kb on - strand, within nlpD at 3578.693 kb on - strand, within nlpD at 3578.711 kb on - strand, within nlpD at 3578.958 kb on + strand, within nlpD at 3578.966 kb on - strand, within nlpD at 3579.092 kb on + strand, within nlpD at 3579.163 kb on - strand at 3579.185 kb on - strand at 3579.266 kb on + strand at 3579.316 kb on + strand at 3579.322 kb on - strand at 3579.324 kb on - strand at 3579.546 kb on + strand, within pcm at 3579.587 kb on - strand, within pcm at 3579.640 kb on + strand, within pcm at 3579.655 kb on - strand, within pcm at 3579.721 kb on - strand, within pcm at 3579.736 kb on + strand, within pcm at 3579.744 kb on - strand, within pcm at 3579.750 kb on - strand, within pcm at 3580.043 kb on + strand, within surE at 3580.141 kb on + strand, within surE
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 100 mM remove 3,577,344 - -0.4 3,577,448 + rpoS SO3432 0.19 +1.4 3,577,456 - rpoS SO3432 0.20 +0.2 3,577,495 + rpoS SO3432 0.24 -0.7 3,577,528 - rpoS SO3432 0.28 -0.9 3,577,552 + rpoS SO3432 0.30 +1.0 3,577,626 + rpoS SO3432 0.38 -0.0 3,577,634 - rpoS SO3432 0.38 +0.3 3,577,634 - rpoS SO3432 0.38 +0.1 3,577,639 + rpoS SO3432 0.39 -0.3 3,577,640 - rpoS SO3432 0.39 -0.2 3,577,721 + rpoS SO3432 0.47 -0.2 3,577,729 - rpoS SO3432 0.48 +0.2 3,577,765 + rpoS SO3432 0.52 -0.2 3,577,956 + rpoS SO3432 0.71 +0.8 3,578,074 - rpoS SO3432 0.83 -0.2 3,578,168 + -0.6 3,578,338 + -2.6 3,578,343 - -0.3 3,578,353 - +1.2 3,578,394 + -0.9 3,578,402 - +0.3 3,578,464 + nlpD SO3433 0.16 -2.5 3,578,464 + nlpD SO3433 0.16 -1.5 3,578,642 - nlpD SO3433 0.36 -0.9 3,578,685 + nlpD SO3433 0.41 -1.4 3,578,685 + nlpD SO3433 0.41 -1.6 3,578,685 + nlpD SO3433 0.41 -1.0 3,578,693 - nlpD SO3433 0.42 -0.6 3,578,693 - nlpD SO3433 0.42 -1.8 3,578,693 - nlpD SO3433 0.42 -2.5 3,578,711 - nlpD SO3433 0.44 -2.1 3,578,958 + nlpD SO3433 0.71 -1.0 3,578,966 - nlpD SO3433 0.72 -0.6 3,579,092 + nlpD SO3433 0.86 -1.6 3,579,163 - -0.4 3,579,185 - -0.8 3,579,266 + -0.9 3,579,316 + -0.2 3,579,322 - -0.2 3,579,324 - +0.6 3,579,546 + pcm SO3434 0.43 -2.3 3,579,587 - pcm SO3434 0.49 -2.0 3,579,640 + pcm SO3434 0.58 -1.3 3,579,655 - pcm SO3434 0.60 -0.9 3,579,721 - pcm SO3434 0.70 +0.2 3,579,736 + pcm SO3434 0.73 -0.9 3,579,744 - pcm SO3434 0.74 -0.4 3,579,750 - pcm SO3434 0.75 +2.1 3,580,043 + surE SO3435 0.18 -2.8 3,580,141 + surE SO3435 0.31 -2.5
Or see this region's nucleotide sequence