Experiment: L-Lysine 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2736 and bioD are separated by 82 nucleotides bioD and bioC are separated by 54 nucleotides
SO2736: SO2736 - conserved hypothetical protein (NCBI ptt file), at 2,851,535 to 2,852,503
SO2736
SO2737: bioD - dethiobiotin synthase (NCBI ptt file), at 2,852,586 to 2,853,281
bioD
SO2738: bioC - biotin synthesis protein BioC (NCBI ptt file), at 2,853,336 to 2,854,163
bioC
Position (kb)
2852
2853
2854 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 2851.668 kb on + strand, within SO2736 at 2851.668 kb on + strand, within SO2736 at 2851.668 kb on + strand, within SO2736 at 2851.676 kb on - strand, within SO2736 at 2851.805 kb on + strand, within SO2736 at 2851.885 kb on - strand, within SO2736 at 2851.885 kb on - strand, within SO2736 at 2851.885 kb on - strand, within SO2736 at 2851.988 kb on - strand, within SO2736 at 2852.003 kb on + strand, within SO2736 at 2852.022 kb on - strand, within SO2736 at 2852.068 kb on + strand, within SO2736 at 2852.092 kb on + strand, within SO2736 at 2852.109 kb on + strand, within SO2736 at 2852.126 kb on + strand, within SO2736 at 2852.126 kb on - strand, within SO2736 at 2852.134 kb on - strand, within SO2736 at 2852.213 kb on + strand, within SO2736 at 2852.218 kb on + strand, within SO2736 at 2852.218 kb on + strand, within SO2736 at 2852.220 kb on + strand, within SO2736 at 2852.226 kb on - strand, within SO2736 at 2852.226 kb on - strand, within SO2736 at 2852.226 kb on - strand, within SO2736 at 2852.228 kb on - strand, within SO2736 at 2852.245 kb on - strand, within SO2736 at 2852.251 kb on - strand, within SO2736 at 2852.278 kb on - strand, within SO2736 at 2852.311 kb on + strand, within SO2736 at 2852.315 kb on + strand, within SO2736 at 2852.319 kb on - strand, within SO2736 at 2852.329 kb on - strand, within SO2736 at 2852.334 kb on - strand, within SO2736 at 2852.340 kb on - strand, within SO2736 at 2852.346 kb on - strand, within SO2736 at 2852.412 kb on + strand at 2852.412 kb on + strand at 2852.412 kb on + strand at 2852.458 kb on - strand at 2852.505 kb on - strand at 2853.392 kb on - strand at 2853.393 kb on - strand at 2853.845 kb on - strand, within bioC at 2853.885 kb on - strand, within bioC at 2853.980 kb on - strand, within bioC at 2854.231 kb on - strand at 2854.277 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 100 mM remove 2,851,668 + SO2736 0.14 -0.5 2,851,668 + SO2736 0.14 -1.3 2,851,668 + SO2736 0.14 +0.6 2,851,676 - SO2736 0.15 +1.3 2,851,805 + SO2736 0.28 +0.4 2,851,885 - SO2736 0.36 +1.6 2,851,885 - SO2736 0.36 +0.2 2,851,885 - SO2736 0.36 -0.0 2,851,988 - SO2736 0.47 -0.1 2,852,003 + SO2736 0.48 +0.4 2,852,022 - SO2736 0.50 +1.2 2,852,068 + SO2736 0.55 +0.0 2,852,092 + SO2736 0.57 +0.2 2,852,109 + SO2736 0.59 +0.4 2,852,126 + SO2736 0.61 +0.6 2,852,126 - SO2736 0.61 +0.7 2,852,134 - SO2736 0.62 -0.3 2,852,213 + SO2736 0.70 +5.0 2,852,218 + SO2736 0.70 +0.2 2,852,218 + SO2736 0.70 +0.5 2,852,220 + SO2736 0.71 +0.2 2,852,226 - SO2736 0.71 +0.1 2,852,226 - SO2736 0.71 +0.5 2,852,226 - SO2736 0.71 +0.2 2,852,228 - SO2736 0.72 -0.4 2,852,245 - SO2736 0.73 +1.3 2,852,251 - SO2736 0.74 +0.9 2,852,278 - SO2736 0.77 +0.4 2,852,311 + SO2736 0.80 +0.4 2,852,315 + SO2736 0.80 +0.4 2,852,319 - SO2736 0.81 +0.5 2,852,329 - SO2736 0.82 +0.3 2,852,334 - SO2736 0.82 -0.1 2,852,340 - SO2736 0.83 +0.6 2,852,346 - SO2736 0.84 +1.9 2,852,412 + +0.3 2,852,412 + -0.0 2,852,412 + +1.1 2,852,458 - -0.1 2,852,505 - -1.4 2,853,392 - +0.9 2,853,393 - +0.8 2,853,845 - bioC SO2738 0.61 +0.1 2,853,885 - bioC SO2738 0.66 -0.7 2,853,980 - bioC SO2738 0.78 -0.6 2,854,231 - +0.6 2,854,277 - +0.4
Or see this region's nucleotide sequence