Experiment: L-Lysine 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspL and gspM are separated by 1 nucleotides gspM and gspN are separated by 14 nucleotides gspN and SO0177 are separated by 59 nucleotides
SO0174: gspL - general secretion pathway protein L (NCBI ptt file), at 180,434 to 181,621
gspL
SO0175: gspM - general secretion pathway protein M (NCBI ptt file), at 181,623 to 182,099
gspM
SO0176: gspN - general secretion pathway protein N (NCBI ptt file), at 182,114 to 182,875
gspN
SO0177: SO0177 - HAD-superfamily hydrolase, subfamily IA, variant 3 protein family (NCBI ptt file), at 182,935 to 183,597
SO0177
Position (kb) 181
182
183 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 180.628 kb on - strand, within gspL at 180.628 kb on - strand, within gspL at 180.697 kb on - strand, within gspL at 180.910 kb on - strand, within gspL at 180.920 kb on + strand, within gspL at 180.932 kb on + strand, within gspL at 181.035 kb on + strand, within gspL at 181.078 kb on - strand, within gspL at 181.147 kb on - strand, within gspL at 181.225 kb on - strand, within gspL at 181.281 kb on - strand, within gspL at 181.311 kb on - strand, within gspL at 181.364 kb on + strand, within gspL at 181.364 kb on + strand, within gspL at 181.372 kb on - strand, within gspL at 181.372 kb on - strand, within gspL at 181.404 kb on - strand, within gspL at 181.416 kb on + strand, within gspL at 181.591 kb on - strand at 181.604 kb on + strand at 181.612 kb on - strand at 181.651 kb on + strand at 181.659 kb on - strand at 181.659 kb on - strand at 181.659 kb on - strand at 181.734 kb on + strand, within gspM at 181.754 kb on - strand, within gspM at 181.798 kb on + strand, within gspM at 181.881 kb on - strand, within gspM at 182.067 kb on + strand at 182.081 kb on + strand at 182.104 kb on + strand at 182.149 kb on + strand at 182.149 kb on + strand at 182.152 kb on - strand at 182.171 kb on + strand at 182.229 kb on + strand, within gspN at 182.319 kb on + strand, within gspN at 182.404 kb on - strand, within gspN at 182.420 kb on + strand, within gspN at 182.425 kb on - strand, within gspN at 182.548 kb on + strand, within gspN at 182.626 kb on + strand, within gspN at 182.637 kb on + strand, within gspN at 182.645 kb on - strand, within gspN at 182.722 kb on + strand, within gspN at 182.748 kb on - strand, within gspN at 182.758 kb on + strand, within gspN at 182.766 kb on - strand, within gspN at 182.787 kb on - strand, within gspN at 182.827 kb on + strand at 182.869 kb on + strand at 182.966 kb on - strand at 183.038 kb on - strand, within SO0177 at 183.051 kb on - strand, within SO0177
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 100 mM remove 180,628 - gspL SO0174 0.16 -1.7 180,628 - gspL SO0174 0.16 -0.9 180,697 - gspL SO0174 0.22 -1.4 180,910 - gspL SO0174 0.40 -2.8 180,920 + gspL SO0174 0.41 -1.5 180,932 + gspL SO0174 0.42 +0.1 181,035 + gspL SO0174 0.51 -2.2 181,078 - gspL SO0174 0.54 -2.0 181,147 - gspL SO0174 0.60 +0.2 181,225 - gspL SO0174 0.67 -1.4 181,281 - gspL SO0174 0.71 -2.2 181,311 - gspL SO0174 0.74 -0.6 181,364 + gspL SO0174 0.78 -0.3 181,364 + gspL SO0174 0.78 -0.5 181,372 - gspL SO0174 0.79 -0.9 181,372 - gspL SO0174 0.79 -0.4 181,404 - gspL SO0174 0.82 -2.1 181,416 + gspL SO0174 0.83 -3.3 181,591 - -1.6 181,604 + -0.6 181,612 - -0.5 181,651 + -2.6 181,659 - -1.2 181,659 - +0.0 181,659 - -0.9 181,734 + gspM SO0175 0.23 -1.0 181,754 - gspM SO0175 0.27 -1.1 181,798 + gspM SO0175 0.37 -2.2 181,881 - gspM SO0175 0.54 -2.4 182,067 + -1.1 182,081 + -0.5 182,104 + -0.7 182,149 + -1.6 182,149 + -1.6 182,152 - -0.6 182,171 + -1.7 182,229 + gspN SO0176 0.15 -1.4 182,319 + gspN SO0176 0.27 -3.3 182,404 - gspN SO0176 0.38 -2.8 182,420 + gspN SO0176 0.40 -0.8 182,425 - gspN SO0176 0.41 -0.9 182,548 + gspN SO0176 0.57 -1.1 182,626 + gspN SO0176 0.67 -1.2 182,637 + gspN SO0176 0.69 -0.5 182,645 - gspN SO0176 0.70 -0.4 182,722 + gspN SO0176 0.80 -1.5 182,748 - gspN SO0176 0.83 -1.0 182,758 + gspN SO0176 0.85 +0.4 182,766 - gspN SO0176 0.86 -1.6 182,787 - gspN SO0176 0.88 +0.1 182,827 + -0.6 182,869 + +0.1 182,966 - +0.4 183,038 - SO0177 0.16 -1.2 183,051 - SO0177 0.17 -0.6
Or see this region's nucleotide sequence