Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rep and SO4326 are separated by 283 nucleotides SO4326 and SO4327 are separated by 25 nucleotides SO4327 and SO4327.1 are separated by 13 nucleotides
SO4325: rep - ATP-dependent DNA helicase Rep (NCBI ptt file), at 4,515,491 to 4,517,506
rep
SO4326: SO4326 - transcriptional regulator, TetR family (NCBI ptt file), at 4,517,790 to 4,518,398
SO4326
SO4327: SO4327 - HlyD family secretion domain protein (NCBI ptt file), at 4,518,424 to 4,519,542
SO4327
SO4327.1: SO4327.1 - hypothetical multidrug resistance protein (NCBI ptt file), at 4,519,556 to 4,521,445
SO4327.1
Position (kb)
4518
4519
4520 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4517.535 kb on - strand at 4517.536 kb on - strand at 4517.536 kb on - strand at 4517.597 kb on - strand at 4517.599 kb on - strand at 4517.614 kb on + strand at 4517.657 kb on + strand at 4517.665 kb on - strand at 4517.983 kb on + strand, within SO4326 at 4517.983 kb on + strand, within SO4326 at 4517.991 kb on - strand, within SO4326 at 4517.993 kb on + strand, within SO4326 at 4517.993 kb on + strand, within SO4326 at 4517.993 kb on + strand, within SO4326 at 4517.993 kb on + strand, within SO4326 at 4518.101 kb on - strand, within SO4326 at 4518.132 kb on + strand, within SO4326 at 4518.139 kb on + strand, within SO4326 at 4518.139 kb on + strand, within SO4326 at 4518.147 kb on - strand, within SO4326 at 4518.165 kb on + strand, within SO4326 at 4518.231 kb on + strand, within SO4326 at 4518.245 kb on - strand, within SO4326 at 4518.288 kb on - strand, within SO4326 at 4518.309 kb on + strand, within SO4326 at 4518.315 kb on + strand, within SO4326 at 4518.334 kb on + strand, within SO4326 at 4518.342 kb on - strand at 4518.342 kb on - strand at 4518.342 kb on - strand at 4518.342 kb on - strand at 4518.391 kb on + strand at 4518.468 kb on - strand at 4518.485 kb on + strand at 4518.485 kb on + strand at 4518.485 kb on + strand at 4518.493 kb on - strand at 4518.493 kb on - strand at 4518.493 kb on - strand at 4518.493 kb on - strand at 4518.493 kb on - strand at 4518.493 kb on - strand at 4518.493 kb on - strand at 4518.493 kb on - strand at 4518.493 kb on - strand at 4518.493 kb on - strand at 4518.493 kb on - strand at 4518.636 kb on - strand, within SO4327 at 4518.692 kb on - strand, within SO4327 at 4518.898 kb on - strand, within SO4327 at 4518.928 kb on + strand, within SO4327 at 4518.936 kb on - strand, within SO4327 at 4518.993 kb on - strand, within SO4327 at 4519.006 kb on + strand, within SO4327 at 4519.009 kb on - strand, within SO4327 at 4519.106 kb on - strand, within SO4327 at 4519.137 kb on + strand, within SO4327 at 4519.212 kb on + strand, within SO4327 at 4519.220 kb on - strand, within SO4327 at 4519.252 kb on + strand, within SO4327 at 4519.277 kb on + strand, within SO4327 at 4519.292 kb on + strand, within SO4327 at 4519.300 kb on - strand, within SO4327 at 4519.323 kb on + strand, within SO4327 at 4519.356 kb on + strand, within SO4327 at 4519.356 kb on + strand, within SO4327 at 4519.356 kb on + strand, within SO4327 at 4519.364 kb on - strand, within SO4327 at 4519.364 kb on - strand, within SO4327 at 4519.364 kb on - strand, within SO4327 at 4519.392 kb on - strand, within SO4327 at 4519.421 kb on - strand, within SO4327 at 4519.421 kb on - strand, within SO4327 at 4519.507 kb on + strand at 4519.507 kb on + strand at 4519.507 kb on + strand at 4519.507 kb on + strand at 4519.515 kb on - strand at 4519.515 kb on - strand at 4519.518 kb on + strand at 4519.544 kb on - strand at 4519.589 kb on + strand at 4519.589 kb on + strand at 4519.601 kb on + strand at 4519.616 kb on - strand at 4519.675 kb on + strand at 4519.683 kb on + strand at 4519.702 kb on - strand at 4519.748 kb on - strand, within SO4327.1 at 4519.782 kb on - strand, within SO4327.1 at 4519.797 kb on + strand, within SO4327.1 at 4519.798 kb on + strand, within SO4327.1 at 4519.843 kb on + strand, within SO4327.1 at 4519.851 kb on - strand, within SO4327.1 at 4519.851 kb on - strand, within SO4327.1 at 4519.851 kb on - strand, within SO4327.1 at 4519.851 kb on - strand, within SO4327.1 at 4519.855 kb on + strand, within SO4327.1 at 4519.855 kb on + strand, within SO4327.1 at 4519.863 kb on - strand, within SO4327.1 at 4519.912 kb on + strand, within SO4327.1 at 4519.912 kb on + strand, within SO4327.1 at 4519.933 kb on - strand, within SO4327.1 at 4519.935 kb on + strand, within SO4327.1 at 4519.943 kb on - strand, within SO4327.1 at 4519.962 kb on + strand, within SO4327.1 at 4520.125 kb on + strand, within SO4327.1 at 4520.133 kb on - strand, within SO4327.1 at 4520.147 kb on - strand, within SO4327.1 at 4520.219 kb on + strand, within SO4327.1 at 4520.227 kb on - strand, within SO4327.1 at 4520.227 kb on - strand, within SO4327.1 at 4520.227 kb on - strand, within SO4327.1 at 4520.228 kb on + strand, within SO4327.1 at 4520.236 kb on - strand, within SO4327.1 at 4520.262 kb on + strand, within SO4327.1 at 4520.285 kb on + strand, within SO4327.1 at 4520.374 kb on + strand, within SO4327.1 at 4520.395 kb on + strand, within SO4327.1 at 4520.421 kb on + strand, within SO4327.1 at 4520.423 kb on + strand, within SO4327.1
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM remove 4,517,535 - +0.1 4,517,536 - -0.0 4,517,536 - +0.1 4,517,597 - +0.4 4,517,599 - +0.3 4,517,614 + +0.2 4,517,657 + -0.8 4,517,665 - +0.2 4,517,983 + SO4326 0.32 +1.1 4,517,983 + SO4326 0.32 -0.1 4,517,991 - SO4326 0.33 +0.5 4,517,993 + SO4326 0.33 -0.1 4,517,993 + SO4326 0.33 +0.8 4,517,993 + SO4326 0.33 +0.4 4,517,993 + SO4326 0.33 -1.6 4,518,101 - SO4326 0.51 +0.2 4,518,132 + SO4326 0.56 +0.1 4,518,139 + SO4326 0.57 +0.1 4,518,139 + SO4326 0.57 +0.4 4,518,147 - SO4326 0.59 +0.1 4,518,165 + SO4326 0.62 -0.1 4,518,231 + SO4326 0.72 -0.2 4,518,245 - SO4326 0.75 +0.9 4,518,288 - SO4326 0.82 +0.3 4,518,309 + SO4326 0.85 +0.6 4,518,315 + SO4326 0.86 -0.2 4,518,334 + SO4326 0.89 -0.1 4,518,342 - -0.7 4,518,342 - +0.2 4,518,342 - -0.0 4,518,342 - -0.6 4,518,391 + -0.2 4,518,468 - -0.7 4,518,485 + +0.1 4,518,485 + -0.1 4,518,485 + +1.1 4,518,493 - -0.1 4,518,493 - -0.9 4,518,493 - -1.4 4,518,493 - -1.6 4,518,493 - +0.2 4,518,493 - -0.4 4,518,493 - -0.3 4,518,493 - -0.2 4,518,493 - -0.4 4,518,493 - +0.1 4,518,493 - +0.1 4,518,636 - SO4327 0.19 -0.5 4,518,692 - SO4327 0.24 -0.5 4,518,898 - SO4327 0.42 +1.0 4,518,928 + SO4327 0.45 -0.0 4,518,936 - SO4327 0.46 -0.5 4,518,993 - SO4327 0.51 -0.7 4,519,006 + SO4327 0.52 -0.3 4,519,009 - SO4327 0.52 +0.5 4,519,106 - SO4327 0.61 +0.9 4,519,137 + SO4327 0.64 -0.2 4,519,212 + SO4327 0.70 -0.6 4,519,220 - SO4327 0.71 -1.6 4,519,252 + SO4327 0.74 +0.3 4,519,277 + SO4327 0.76 +0.8 4,519,292 + SO4327 0.78 -0.3 4,519,300 - SO4327 0.78 +0.1 4,519,323 + SO4327 0.80 +0.8 4,519,356 + SO4327 0.83 -0.6 4,519,356 + SO4327 0.83 -0.0 4,519,356 + SO4327 0.83 +0.3 4,519,364 - SO4327 0.84 -0.9 4,519,364 - SO4327 0.84 +1.9 4,519,364 - SO4327 0.84 +0.3 4,519,392 - SO4327 0.87 -0.1 4,519,421 - SO4327 0.89 -0.3 4,519,421 - SO4327 0.89 +0.0 4,519,507 + +0.7 4,519,507 + -0.1 4,519,507 + -0.3 4,519,507 + +0.1 4,519,515 - -0.8 4,519,515 - -1.2 4,519,518 + +0.6 4,519,544 - -0.1 4,519,589 + +0.7 4,519,589 + +0.5 4,519,601 + -0.9 4,519,616 - +0.5 4,519,675 + -0.5 4,519,683 + +0.1 4,519,702 - +0.0 4,519,748 - SO4327.1 0.10 +0.2 4,519,782 - SO4327.1 0.12 -0.8 4,519,797 + SO4327.1 0.13 -0.1 4,519,798 + SO4327.1 0.13 -0.8 4,519,843 + SO4327.1 0.15 -0.2 4,519,851 - SO4327.1 0.16 -0.3 4,519,851 - SO4327.1 0.16 +0.4 4,519,851 - SO4327.1 0.16 +0.3 4,519,851 - SO4327.1 0.16 -2.7 4,519,855 + SO4327.1 0.16 +0.8 4,519,855 + SO4327.1 0.16 +0.6 4,519,863 - SO4327.1 0.16 -0.0 4,519,912 + SO4327.1 0.19 -0.1 4,519,912 + SO4327.1 0.19 -0.4 4,519,933 - SO4327.1 0.20 +0.1 4,519,935 + SO4327.1 0.20 +0.3 4,519,943 - SO4327.1 0.20 +0.4 4,519,962 + SO4327.1 0.21 -0.3 4,520,125 + SO4327.1 0.30 +0.2 4,520,133 - SO4327.1 0.31 -0.2 4,520,147 - SO4327.1 0.31 -0.3 4,520,219 + SO4327.1 0.35 +0.0 4,520,227 - SO4327.1 0.36 -0.2 4,520,227 - SO4327.1 0.36 +0.4 4,520,227 - SO4327.1 0.36 +1.0 4,520,228 + SO4327.1 0.36 -0.9 4,520,236 - SO4327.1 0.36 -0.4 4,520,262 + SO4327.1 0.37 -0.7 4,520,285 + SO4327.1 0.39 +0.1 4,520,374 + SO4327.1 0.43 -0.7 4,520,395 + SO4327.1 0.44 -0.3 4,520,421 + SO4327.1 0.46 +0.0 4,520,423 + SO4327.1 0.46 -0.4
Or see this region's nucleotide sequence