Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt thiS and thiF overlap by 4 nucleotides thiF and thiDE overlap by 4 nucleotides thiDE and thiC overlap by 1 nucleotides  
        
        SO2442: thiS - thiS protein, putative (NCBI ptt file), at 2,555,858 to 2,556,061 
         
        thiS 
         
        
        SO2443: thiF - thiF protein, putative (NCBI ptt file), at 2,556,058 to 2,556,960 
         
        thiF 
         
        
        SO2444: thiDE - phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase, putative (NCBI ptt file), at 2,556,957 to 2,558,537 
         
        thiDE 
         
        
        SO2445: thiC - thiamin biosynthesis protein ThiC (NCBI ptt file), at 2,558,537 to 2,560,702 
         
        thiC 
         Position (kb)  
2556 
 
2557 
 
2558 
 
2559 Strain fitness (log2 ratio)  
-1 
 
0 
 
1 at 2556.483 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.738 kb on + strand, within thiF at 2556.738 kb on + strand, within thiF at 2556.918 kb on - strand at 2556.971 kb on - strand at 2557.138 kb on - strand, within thiDE at 2557.138 kb on - strand, within thiDE at 2557.214 kb on - strand, within thiDE at 2557.280 kb on + strand, within thiDE at 2557.413 kb on - strand, within thiDE at 2557.483 kb on + strand, within thiDE at 2557.552 kb on + strand, within thiDE at 2557.555 kb on - strand, within thiDE at 2557.676 kb on + strand, within thiDE at 2557.690 kb on + strand, within thiDE at 2557.723 kb on + strand, within thiDE at 2557.729 kb on + strand, within thiDE at 2557.742 kb on + strand, within thiDE at 2557.760 kb on - strand, within thiDE at 2557.770 kb on + strand, within thiDE at 2557.778 kb on - strand, within thiDE at 2557.782 kb on + strand, within thiDE at 2557.790 kb on - strand, within thiDE at 2557.794 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE at 2558.275 kb on - strand, within thiDE at 2558.316 kb on - strand, within thiDE at 2558.535 kb on + strand at 2558.543 kb on - strand at 2558.580 kb on + strand at 2558.613 kb on + strand at 2558.615 kb on - strand at 2558.863 kb on - strand, within thiC at 2558.898 kb on - strand, within thiC at 2558.905 kb on - strand, within thiC at 2559.185 kb on + strand, within thiC at 2559.193 kb on - strand, within thiC at 2559.193 kb on - strand, within thiC at 2559.278 kb on - strand, within thiC at 2559.302 kb on - strand, within thiC at 2559.362 kb on + strand, within thiC at 2559.408 kb on + strand, within thiC  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   1-ethyl-3-methylimidazolium chloride 40 mM             remove   2,556,483   +  thiF  SO2443   0.47  -0.4   2,556,680   +  thiF  SO2443   0.69  +0.0   2,556,680   +  thiF  SO2443   0.69  -0.5   2,556,680   +  thiF  SO2443   0.69  +0.2   2,556,738   +  thiF  SO2443   0.75  +0.6   2,556,738   +  thiF  SO2443   0.75  +0.0   2,556,918   -        -1.1   2,556,971   -        +0.4   2,557,138   -  thiDE  SO2444   0.11  +0.3   2,557,138   -  thiDE  SO2444   0.11  -0.4   2,557,214   -  thiDE  SO2444   0.16  +0.7   2,557,280   +  thiDE  SO2444   0.20  -0.4   2,557,413   -  thiDE  SO2444   0.29  +0.4   2,557,483   +  thiDE  SO2444   0.33  -0.4   2,557,552   +  thiDE  SO2444   0.38  -0.3   2,557,555   -  thiDE  SO2444   0.38  +0.5   2,557,676   +  thiDE  SO2444   0.45  -0.2   2,557,690   +  thiDE  SO2444   0.46  +0.4   2,557,723   +  thiDE  SO2444   0.48  -0.1   2,557,729   +  thiDE  SO2444   0.49  -1.1   2,557,742   +  thiDE  SO2444   0.50  +0.3   2,557,760   -  thiDE  SO2444   0.51  -0.7   2,557,770   +  thiDE  SO2444   0.51  +0.5   2,557,778   -  thiDE  SO2444   0.52  +0.4   2,557,782   +  thiDE  SO2444   0.52  +0.3   2,557,790   -  thiDE  SO2444   0.53  -0.4   2,557,794   +  thiDE  SO2444   0.53  +0.2   2,557,874   +  thiDE  SO2444   0.58  +1.2   2,557,874   +  thiDE  SO2444   0.58  -0.1   2,558,275   -  thiDE  SO2444   0.83  -0.2   2,558,316   -  thiDE  SO2444   0.86  -0.8   2,558,535   +        -1.1   2,558,543   -        +0.4   2,558,580   +        -0.2   2,558,613   +        -1.0   2,558,615   -        -0.2   2,558,863   -  thiC  SO2445   0.15  -0.2   2,558,898   -  thiC  SO2445   0.17  -0.3   2,558,905   -  thiC  SO2445   0.17  +1.3   2,559,185   +  thiC  SO2445   0.30  +0.1   2,559,193   -  thiC  SO2445   0.30  +0.5   2,559,193   -  thiC  SO2445   0.30  +0.1   2,559,278   -  thiC  SO2445   0.34  -0.7   2,559,302   -  thiC  SO2445   0.35  -0.1   2,559,362   +  thiC  SO2445   0.38  +0.2   2,559,408   +  thiC  SO2445   0.40  -0.5 
 
Or see this region's nucleotide sequence