Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3741.1 and SO3743 are separated by 78 nucleotides SO3743 and SO3744 are separated by 153 nucleotides SO3744 and rfaE overlap by 4 nucleotides
SO3741.1: SO3741.1 - hypothetical protein (NCBI ptt file), at 3,888,093 to 3,888,482
SO3741.1
SO3743: SO3743 - transcriptional regulator, TetR family (NCBI ptt file), at 3,888,561 to 3,889,040
SO3743
SO3744: SO3744 - hypothetical protein (NCBI ptt file), at 3,889,194 to 3,890,021
SO3744
SO3745: rfaE - ADP-heptose synthase (NCBI ptt file), at 3,890,018 to 3,891,448
rfaE
Position (kb)
3889
3890
3891 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3888.286 kb on + strand, within SO3741.1 at 3888.286 kb on + strand, within SO3741.1 at 3888.386 kb on + strand, within SO3741.1 at 3888.404 kb on - strand, within SO3741.1 at 3888.418 kb on + strand, within SO3741.1 at 3888.484 kb on - strand at 3888.498 kb on + strand at 3888.682 kb on - strand, within SO3743 at 3888.682 kb on - strand, within SO3743 at 3888.696 kb on - strand, within SO3743 at 3888.696 kb on - strand, within SO3743 at 3888.696 kb on - strand, within SO3743 at 3888.696 kb on - strand, within SO3743 at 3888.749 kb on - strand, within SO3743 at 3888.754 kb on - strand, within SO3743 at 3888.756 kb on + strand, within SO3743 at 3888.756 kb on + strand, within SO3743 at 3888.756 kb on + strand, within SO3743 at 3888.764 kb on - strand, within SO3743 at 3888.901 kb on - strand, within SO3743 at 3888.945 kb on - strand, within SO3743 at 3888.945 kb on - strand, within SO3743 at 3889.018 kb on - strand at 3889.216 kb on + strand at 3889.216 kb on + strand at 3889.248 kb on + strand at 3889.346 kb on + strand, within SO3744 at 3889.487 kb on + strand, within SO3744 at 3889.491 kb on + strand, within SO3744 at 3889.495 kb on - strand, within SO3744 at 3889.516 kb on + strand, within SO3744 at 3889.532 kb on + strand, within SO3744 at 3889.540 kb on - strand, within SO3744 at 3889.602 kb on + strand, within SO3744 at 3889.685 kb on + strand, within SO3744 at 3889.861 kb on + strand, within SO3744 at 3889.866 kb on + strand, within SO3744 at 3889.869 kb on - strand, within SO3744 at 3889.869 kb on - strand, within SO3744 at 3889.908 kb on + strand, within SO3744 at 3889.911 kb on - strand, within SO3744 at 3889.916 kb on - strand, within SO3744 at 3890.081 kb on + strand at 3890.081 kb on + strand at 3890.196 kb on + strand, within rfaE at 3890.196 kb on + strand, within rfaE at 3890.196 kb on + strand, within rfaE at 3890.196 kb on + strand, within rfaE at 3890.196 kb on + strand, within rfaE at 3890.234 kb on + strand, within rfaE at 3890.509 kb on + strand, within rfaE at 3890.517 kb on + strand, within rfaE at 3890.527 kb on + strand, within rfaE at 3890.558 kb on - strand, within rfaE at 3890.566 kb on + strand, within rfaE at 3890.608 kb on - strand, within rfaE at 3890.665 kb on - strand, within rfaE at 3890.743 kb on - strand, within rfaE at 3890.759 kb on + strand, within rfaE at 3890.759 kb on + strand, within rfaE at 3890.794 kb on - strand, within rfaE at 3890.910 kb on + strand, within rfaE
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 3,888,286 + SO3741.1 0.49 -0.1 3,888,286 + SO3741.1 0.49 -0.0 3,888,386 + SO3741.1 0.75 -0.4 3,888,404 - SO3741.1 0.80 +0.7 3,888,418 + SO3741.1 0.83 +0.3 3,888,484 - -0.5 3,888,498 + -0.2 3,888,682 - SO3743 0.25 -1.1 3,888,682 - SO3743 0.25 -0.1 3,888,696 - SO3743 0.28 -0.2 3,888,696 - SO3743 0.28 -0.2 3,888,696 - SO3743 0.28 -0.2 3,888,696 - SO3743 0.28 -1.2 3,888,749 - SO3743 0.39 +0.5 3,888,754 - SO3743 0.40 -0.5 3,888,756 + SO3743 0.41 -0.6 3,888,756 + SO3743 0.41 +0.2 3,888,756 + SO3743 0.41 -2.2 3,888,764 - SO3743 0.42 +0.1 3,888,901 - SO3743 0.71 -0.6 3,888,945 - SO3743 0.80 +0.3 3,888,945 - SO3743 0.80 -0.6 3,889,018 - -0.2 3,889,216 + +0.3 3,889,216 + +0.1 3,889,248 + +1.2 3,889,346 + SO3744 0.18 -1.5 3,889,487 + SO3744 0.35 -0.2 3,889,491 + SO3744 0.36 -1.5 3,889,495 - SO3744 0.36 -0.5 3,889,516 + SO3744 0.39 -0.3 3,889,532 + SO3744 0.41 -1.0 3,889,540 - SO3744 0.42 +0.2 3,889,602 + SO3744 0.49 -2.2 3,889,685 + SO3744 0.59 +0.0 3,889,861 + SO3744 0.81 -0.6 3,889,866 + SO3744 0.81 +0.4 3,889,869 - SO3744 0.82 -1.2 3,889,869 - SO3744 0.82 +0.1 3,889,908 + SO3744 0.86 -0.8 3,889,911 - SO3744 0.87 +0.0 3,889,916 - SO3744 0.87 -0.2 3,890,081 + +2.0 3,890,081 + +2.1 3,890,196 + rfaE SO3745 0.12 +1.8 3,890,196 + rfaE SO3745 0.12 +2.2 3,890,196 + rfaE SO3745 0.12 +1.3 3,890,196 + rfaE SO3745 0.12 +2.0 3,890,196 + rfaE SO3745 0.12 +2.1 3,890,234 + rfaE SO3745 0.15 +1.9 3,890,509 + rfaE SO3745 0.34 +2.3 3,890,517 + rfaE SO3745 0.35 +2.8 3,890,527 + rfaE SO3745 0.36 +2.9 3,890,558 - rfaE SO3745 0.38 +2.5 3,890,566 + rfaE SO3745 0.38 +2.2 3,890,608 - rfaE SO3745 0.41 +2.5 3,890,665 - rfaE SO3745 0.45 +2.8 3,890,743 - rfaE SO3745 0.51 +2.5 3,890,759 + rfaE SO3745 0.52 +2.5 3,890,759 + rfaE SO3745 0.52 +2.3 3,890,794 - rfaE SO3745 0.54 +2.4 3,890,910 + rfaE SO3745 0.62 +2.4
Or see this region's nucleotide sequence