Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1461 and SO1462 overlap by 4 nucleotides SO1462 and SO1463 overlap by 4 nucleotides
SO1461: SO1461 - protein kinase, putative (NCBI ptt file), at 1,519,669 to 1,523,799
SO1461
SO1462: SO1462 - hypothetical protein (NCBI ptt file), at 1,523,796 to 1,525,442
SO1462
SO1463: SO1463 - hypothetical protein (NCBI ptt file), at 1,525,439 to 1,529,959
SO1463
Position (kb)
1523
1524
1525
1526 Strain fitness (log2 ratio)
-2
-1
0
1 at 1522.843 kb on + strand, within SO1461 at 1522.843 kb on + strand, within SO1461 at 1522.850 kb on + strand, within SO1461 at 1522.850 kb on + strand, within SO1461 at 1522.858 kb on - strand, within SO1461 at 1522.858 kb on - strand, within SO1461 at 1522.938 kb on + strand, within SO1461 at 1522.945 kb on - strand, within SO1461 at 1522.979 kb on + strand, within SO1461 at 1523.015 kb on + strand, within SO1461 at 1523.023 kb on - strand, within SO1461 at 1523.023 kb on - strand, within SO1461 at 1523.125 kb on + strand, within SO1461 at 1523.125 kb on + strand, within SO1461 at 1523.144 kb on + strand, within SO1461 at 1523.144 kb on + strand, within SO1461 at 1523.144 kb on + strand, within SO1461 at 1523.182 kb on - strand, within SO1461 at 1523.182 kb on - strand, within SO1461 at 1523.218 kb on - strand, within SO1461 at 1523.222 kb on - strand, within SO1461 at 1523.248 kb on + strand, within SO1461 at 1523.250 kb on + strand, within SO1461 at 1523.407 kb on + strand at 1523.408 kb on + strand at 1523.497 kb on - strand at 1523.572 kb on + strand at 1523.613 kb on - strand at 1523.613 kb on - strand at 1523.613 kb on - strand at 1523.613 kb on - strand at 1523.758 kb on + strand at 1523.760 kb on + strand at 1523.773 kb on - strand at 1523.787 kb on + strand at 1523.801 kb on - strand at 1523.884 kb on - strand at 1523.899 kb on + strand at 1523.940 kb on - strand at 1523.955 kb on - strand at 1523.965 kb on - strand, within SO1462 at 1524.264 kb on + strand, within SO1462 at 1524.277 kb on + strand, within SO1462 at 1524.310 kb on + strand, within SO1462 at 1524.310 kb on + strand, within SO1462 at 1524.318 kb on - strand, within SO1462 at 1524.318 kb on - strand, within SO1462 at 1524.318 kb on - strand, within SO1462 at 1524.404 kb on - strand, within SO1462 at 1524.433 kb on + strand, within SO1462 at 1524.489 kb on + strand, within SO1462 at 1524.490 kb on - strand, within SO1462 at 1524.527 kb on + strand, within SO1462 at 1524.529 kb on - strand, within SO1462 at 1524.542 kb on + strand, within SO1462 at 1524.548 kb on + strand, within SO1462 at 1524.624 kb on - strand, within SO1462 at 1524.673 kb on - strand, within SO1462 at 1524.726 kb on + strand, within SO1462 at 1524.822 kb on + strand, within SO1462 at 1524.859 kb on - strand, within SO1462 at 1524.874 kb on + strand, within SO1462 at 1524.917 kb on + strand, within SO1462 at 1524.940 kb on + strand, within SO1462 at 1524.948 kb on - strand, within SO1462 at 1525.000 kb on + strand, within SO1462 at 1525.094 kb on + strand, within SO1462 at 1525.145 kb on + strand, within SO1462 at 1525.204 kb on - strand, within SO1462 at 1525.257 kb on - strand, within SO1462 at 1525.297 kb on + strand at 1525.366 kb on + strand at 1525.420 kb on - strand at 1525.485 kb on + strand at 1525.536 kb on + strand at 1525.566 kb on + strand at 1525.588 kb on + strand at 1525.620 kb on + strand at 1525.682 kb on - strand at 1525.698 kb on + strand at 1525.846 kb on + strand at 1525.956 kb on + strand, within SO1463 at 1525.963 kb on + strand, within SO1463 at 1525.971 kb on + strand, within SO1463 at 1526.259 kb on + strand, within SO1463 at 1526.267 kb on - strand, within SO1463 at 1526.413 kb on - strand, within SO1463
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 1,522,843 + SO1461 0.77 +0.1 1,522,843 + SO1461 0.77 +0.1 1,522,850 + SO1461 0.77 +0.3 1,522,850 + SO1461 0.77 -0.5 1,522,858 - SO1461 0.77 -1.0 1,522,858 - SO1461 0.77 -0.4 1,522,938 + SO1461 0.79 -0.4 1,522,945 - SO1461 0.79 -1.6 1,522,979 + SO1461 0.80 -0.9 1,523,015 + SO1461 0.81 -0.7 1,523,023 - SO1461 0.81 -0.5 1,523,023 - SO1461 0.81 -0.0 1,523,125 + SO1461 0.84 -0.6 1,523,125 + SO1461 0.84 -0.5 1,523,144 + SO1461 0.84 -0.0 1,523,144 + SO1461 0.84 -0.5 1,523,144 + SO1461 0.84 -0.1 1,523,182 - SO1461 0.85 -0.1 1,523,182 - SO1461 0.85 +0.3 1,523,218 - SO1461 0.86 -0.4 1,523,222 - SO1461 0.86 -0.5 1,523,248 + SO1461 0.87 -1.1 1,523,250 + SO1461 0.87 -0.1 1,523,407 + +0.0 1,523,408 + -0.2 1,523,497 - -0.8 1,523,572 + +0.2 1,523,613 - -0.4 1,523,613 - -0.6 1,523,613 - -0.6 1,523,613 - -1.5 1,523,758 + -0.3 1,523,760 + -0.6 1,523,773 - -0.5 1,523,787 + -0.2 1,523,801 - -1.8 1,523,884 - -0.3 1,523,899 + -0.8 1,523,940 - -0.3 1,523,955 - +0.4 1,523,965 - SO1462 0.10 -0.5 1,524,264 + SO1462 0.28 -0.1 1,524,277 + SO1462 0.29 +0.5 1,524,310 + SO1462 0.31 -0.4 1,524,310 + SO1462 0.31 -2.0 1,524,318 - SO1462 0.32 +0.4 1,524,318 - SO1462 0.32 -0.1 1,524,318 - SO1462 0.32 -0.6 1,524,404 - SO1462 0.37 -2.1 1,524,433 + SO1462 0.39 +0.8 1,524,489 + SO1462 0.42 -0.2 1,524,490 - SO1462 0.42 -0.9 1,524,527 + SO1462 0.44 -0.2 1,524,529 - SO1462 0.45 +0.5 1,524,542 + SO1462 0.45 -0.4 1,524,548 + SO1462 0.46 +0.3 1,524,624 - SO1462 0.50 -1.6 1,524,673 - SO1462 0.53 -1.6 1,524,726 + SO1462 0.56 -0.6 1,524,822 + SO1462 0.62 -0.4 1,524,859 - SO1462 0.65 +0.5 1,524,874 + SO1462 0.65 -0.9 1,524,917 + SO1462 0.68 +0.2 1,524,940 + SO1462 0.69 -1.4 1,524,948 - SO1462 0.70 -1.4 1,525,000 + SO1462 0.73 -2.4 1,525,094 + SO1462 0.79 -0.5 1,525,145 + SO1462 0.82 +0.5 1,525,204 - SO1462 0.85 -0.6 1,525,257 - SO1462 0.89 -0.8 1,525,297 + -1.4 1,525,366 + -0.6 1,525,420 - -1.1 1,525,485 + +0.3 1,525,536 + -0.4 1,525,566 + +0.7 1,525,588 + -0.6 1,525,620 + -0.8 1,525,682 - -1.1 1,525,698 + -0.9 1,525,846 + -0.6 1,525,956 + SO1463 0.11 -2.3 1,525,963 + SO1463 0.12 -0.7 1,525,971 + SO1463 0.12 -0.8 1,526,259 + SO1463 0.18 +0.6 1,526,267 - SO1463 0.18 +0.6 1,526,413 - SO1463 0.22 -0.2
Or see this region's nucleotide sequence