Strain Fitness in Shewanella oneidensis MR-1 around SO0030

Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrkA and sun are separated by 15 nucleotidessun and fmt are separated by 2 nucleotidesfmt and def-1 are separated by 15 nucleotides SO0029: trkA - potassium uptake protein TrkA (NCBI ptt file), at 33,706 to 35,115 trkA SO0030: sun - sun protein (NCBI ptt file), at 35,131 to 36,417 sun SO0031: fmt - methionyl-tRNA formyltransferase (NCBI ptt file), at 36,420 to 37,376 fmt SO0032: def-1 - polypeptide deformylase (NCBI ptt file), at 37,392 to 37,898 def-1 Position (kb) 35 36 37Strain fitness (log2 ratio) -1 0 1at 34.621 kb on + strand, within trkAat 35.212 kb on - strandat 35.242 kb on - strandat 35.242 kb on - strandat 35.243 kb on + strandat 35.286 kb on - strand, within sunat 35.301 kb on + strand, within sunat 35.663 kb on - strand, within sunat 35.730 kb on + strand, within sunat 35.740 kb on + strand, within sunat 35.764 kb on - strand, within sunat 35.843 kb on + strand, within sunat 35.843 kb on + strand, within sunat 35.915 kb on - strand, within sunat 35.922 kb on + strand, within sunat 35.937 kb on + strand, within sunat 35.937 kb on + strand, within sunat 36.046 kb on + strand, within sunat 36.068 kb on + strand, within sunat 36.120 kb on - strand, within sunat 36.142 kb on + strand, within sunat 36.190 kb on - strand, within sunat 36.227 kb on + strand, within sunat 36.276 kb on + strand, within sunat 36.286 kb on + strand, within sunat 36.286 kb on - strand, within sunat 36.303 kb on - strandat 36.359 kb on - strandat 36.460 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM
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34,621 + trkA SO0029 0.65 -0.2
35,212 - +0.1
35,242 - +0.1
35,242 - +0.0
35,243 + +0.0
35,286 - sun SO0030 0.12 -0.2
35,301 + sun SO0030 0.13 -0.3
35,663 - sun SO0030 0.41 -0.4
35,730 + sun SO0030 0.47 -0.0
35,740 + sun SO0030 0.47 -0.8
35,764 - sun SO0030 0.49 -0.3
35,843 + sun SO0030 0.55 -0.6
35,843 + sun SO0030 0.55 -0.3
35,915 - sun SO0030 0.61 -0.2
35,922 + sun SO0030 0.61 -0.1
35,937 + sun SO0030 0.63 +0.1
35,937 + sun SO0030 0.63 -0.3
36,046 + sun SO0030 0.71 +0.2
36,068 + sun SO0030 0.73 -0.3
36,120 - sun SO0030 0.77 +0.6
36,142 + sun SO0030 0.79 -1.0
36,190 - sun SO0030 0.82 -0.5
36,227 + sun SO0030 0.85 +0.5
36,276 + sun SO0030 0.89 -0.5
36,286 + sun SO0030 0.90 -0.7
36,286 - sun SO0030 0.90 +1.5
36,303 - -0.3
36,359 - -0.9
36,460 - -0.2

Or see this region's nucleotide sequence