Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO_4328 and SO4329 are separated by 116 nucleotides SO4329 and SO4330 are separated by 168 nucleotides SO4330 and SO4331 are separated by 59 nucleotides SO4331 and SO4332 overlap by 4 nucleotides
SO_4328: SO_4328 - no description, at 4,521,426 to 4,522,646
_4328
SO4329: SO4329 - conserved hypothetical protein (NCBI ptt file), at 4,522,763 to 4,523,017
SO4329
SO4330: SO4330 - conserved hypothetical protein (NCBI ptt file), at 4,523,186 to 4,523,518
SO4330
SO4331: SO4331 - hypothetical protein (NCBI ptt file), at 4,523,578 to 4,523,934
SO4331
SO4332: SO4332 - conserved hypothetical protein (NCBI ptt file), at 4,523,931 to 4,525,097
SO4332
Position (kb)
4522
4523
4524 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4521.805 kb on + strand, within SO_4328 at 4521.909 kb on + strand, within SO_4328 at 4521.978 kb on + strand, within SO_4328 at 4521.986 kb on - strand, within SO_4328 at 4522.162 kb on + strand, within SO_4328 at 4522.242 kb on + strand, within SO_4328 at 4522.320 kb on + strand, within SO_4328 at 4522.367 kb on + strand, within SO_4328 at 4522.367 kb on + strand, within SO_4328 at 4522.367 kb on + strand, within SO_4328 at 4522.493 kb on + strand, within SO_4328 at 4522.632 kb on + strand at 4522.685 kb on - strand at 4523.107 kb on + strand at 4523.107 kb on + strand at 4523.107 kb on + strand at 4523.115 kb on - strand at 4523.115 kb on - strand at 4523.115 kb on - strand at 4523.115 kb on - strand at 4523.115 kb on - strand at 4523.115 kb on - strand at 4523.202 kb on + strand at 4523.216 kb on + strand at 4523.224 kb on - strand, within SO4330 at 4523.226 kb on + strand, within SO4330 at 4523.256 kb on + strand, within SO4330 at 4523.256 kb on + strand, within SO4330 at 4523.256 kb on + strand, within SO4330 at 4523.304 kb on + strand, within SO4330 at 4523.304 kb on + strand, within SO4330 at 4523.304 kb on + strand, within SO4330 at 4523.320 kb on - strand, within SO4330 at 4523.333 kb on + strand, within SO4330 at 4523.538 kb on + strand at 4523.566 kb on + strand at 4523.609 kb on - strand at 4523.800 kb on - strand, within SO4331 at 4523.899 kb on + strand at 4523.907 kb on - strand at 4523.924 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 4,521,805 + SO_4328 0.31 -0.9 4,521,909 + SO_4328 0.40 +0.6 4,521,978 + SO_4328 0.45 -1.0 4,521,986 - SO_4328 0.46 +0.4 4,522,162 + SO_4328 0.60 -0.1 4,522,242 + SO_4328 0.67 -0.4 4,522,320 + SO_4328 0.73 -0.5 4,522,367 + SO_4328 0.77 -0.7 4,522,367 + SO_4328 0.77 -0.4 4,522,367 + SO_4328 0.77 -1.2 4,522,493 + SO_4328 0.87 +0.1 4,522,632 + -0.8 4,522,685 - -0.4 4,523,107 + +1.3 4,523,107 + -0.8 4,523,107 + +0.1 4,523,115 - +0.3 4,523,115 - -0.2 4,523,115 - +0.3 4,523,115 - +0.3 4,523,115 - -0.5 4,523,115 - -1.0 4,523,202 + -3.9 4,523,216 + -1.3 4,523,224 - SO4330 0.11 -0.6 4,523,226 + SO4330 0.12 +0.1 4,523,256 + SO4330 0.21 -0.7 4,523,256 + SO4330 0.21 -0.2 4,523,256 + SO4330 0.21 -1.6 4,523,304 + SO4330 0.35 -0.1 4,523,304 + SO4330 0.35 +0.6 4,523,304 + SO4330 0.35 -2.3 4,523,320 - SO4330 0.40 +0.2 4,523,333 + SO4330 0.44 +0.3 4,523,538 + +1.2 4,523,566 + +0.1 4,523,609 - +0.3 4,523,800 - SO4331 0.62 -0.4 4,523,899 + +0.5 4,523,907 - -0.4 4,523,924 + +0.6
Or see this region's nucleotide sequence