Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheA and pepA-2 are separated by 370 nucleotides pepA-2 and SO1369 are separated by 255 nucleotides
SO1367: pheA - chorismate mutase/prephenate dehydratase (NCBI ptt file), at 1,422,953 to 1,424,968
pheA
SO1368: pepA-2 - cytosol aminopeptidase (NCBI ptt file), at 1,425,339 to 1,426,847
pepA-2
SO1369: SO1369 - conserved hypothetical protein (NCBI ptt file), at 1,427,103 to 1,428,215
SO1369
Position (kb) 1425
1426
1427 Strain fitness (log2 ratio) -1
0
1
2
3 at 1424.352 kb on + strand, within pheA at 1424.352 kb on + strand, within pheA at 1424.360 kb on - strand, within pheA at 1424.362 kb on + strand, within pheA at 1424.453 kb on + strand, within pheA at 1424.453 kb on + strand, within pheA at 1424.461 kb on - strand, within pheA at 1424.649 kb on + strand, within pheA at 1424.816 kb on + strand at 1424.840 kb on + strand at 1424.866 kb on + strand at 1425.023 kb on + strand at 1425.130 kb on + strand at 1425.138 kb on - strand at 1425.143 kb on + strand at 1425.370 kb on + strand at 1425.424 kb on + strand at 1425.625 kb on + strand, within pepA-2 at 1425.633 kb on - strand, within pepA-2 at 1425.635 kb on + strand, within pepA-2 at 1425.638 kb on - strand, within pepA-2 at 1425.671 kb on - strand, within pepA-2 at 1425.703 kb on + strand, within pepA-2 at 1425.713 kb on + strand, within pepA-2 at 1425.713 kb on + strand, within pepA-2 at 1425.721 kb on - strand, within pepA-2 at 1425.767 kb on - strand, within pepA-2 at 1425.809 kb on + strand, within pepA-2 at 1425.859 kb on + strand, within pepA-2 at 1425.859 kb on + strand, within pepA-2 at 1425.893 kb on - strand, within pepA-2 at 1425.948 kb on + strand, within pepA-2 at 1425.987 kb on + strand, within pepA-2 at 1426.337 kb on + strand, within pepA-2 at 1426.337 kb on + strand, within pepA-2 at 1426.345 kb on - strand, within pepA-2 at 1426.406 kb on - strand, within pepA-2 at 1426.434 kb on + strand, within pepA-2 at 1426.445 kb on + strand, within pepA-2 at 1426.626 kb on - strand, within pepA-2 at 1426.641 kb on - strand, within pepA-2 at 1426.683 kb on - strand, within pepA-2 at 1426.704 kb on + strand at 1426.727 kb on - strand at 1426.756 kb on + strand at 1426.844 kb on - strand at 1426.861 kb on - strand at 1427.006 kb on + strand at 1427.006 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 1,424,352 + pheA SO1367 0.69 +0.2 1,424,352 + pheA SO1367 0.69 +1.0 1,424,360 - pheA SO1367 0.70 +0.2 1,424,362 + pheA SO1367 0.70 +0.0 1,424,453 + pheA SO1367 0.74 -1.5 1,424,453 + pheA SO1367 0.74 +0.2 1,424,461 - pheA SO1367 0.75 +0.3 1,424,649 + pheA SO1367 0.84 +0.3 1,424,816 + +0.0 1,424,840 + +0.0 1,424,866 + -0.6 1,425,023 + -0.5 1,425,130 + +0.4 1,425,138 - -0.8 1,425,143 + +0.2 1,425,370 + +2.6 1,425,424 + +3.0 1,425,625 + pepA-2 SO1368 0.19 +2.8 1,425,633 - pepA-2 SO1368 0.19 +2.9 1,425,635 + pepA-2 SO1368 0.20 +2.7 1,425,638 - pepA-2 SO1368 0.20 +3.3 1,425,671 - pepA-2 SO1368 0.22 +2.9 1,425,703 + pepA-2 SO1368 0.24 +3.0 1,425,713 + pepA-2 SO1368 0.25 +1.8 1,425,713 + pepA-2 SO1368 0.25 +2.6 1,425,721 - pepA-2 SO1368 0.25 +3.2 1,425,767 - pepA-2 SO1368 0.28 +2.6 1,425,809 + pepA-2 SO1368 0.31 +2.8 1,425,859 + pepA-2 SO1368 0.34 +2.9 1,425,859 + pepA-2 SO1368 0.34 +2.9 1,425,893 - pepA-2 SO1368 0.37 +3.2 1,425,948 + pepA-2 SO1368 0.40 +2.8 1,425,987 + pepA-2 SO1368 0.43 +2.9 1,426,337 + pepA-2 SO1368 0.66 +2.7 1,426,337 + pepA-2 SO1368 0.66 +3.2 1,426,345 - pepA-2 SO1368 0.67 +3.1 1,426,406 - pepA-2 SO1368 0.71 +3.1 1,426,434 + pepA-2 SO1368 0.73 +3.2 1,426,445 + pepA-2 SO1368 0.73 +0.6 1,426,626 - pepA-2 SO1368 0.85 +2.6 1,426,641 - pepA-2 SO1368 0.86 +2.9 1,426,683 - pepA-2 SO1368 0.89 +3.1 1,426,704 + +2.2 1,426,727 - +2.7 1,426,756 + +2.8 1,426,844 - +3.1 1,426,861 - +2.8 1,427,006 + +2.7 1,427,006 + +2.8
Or see this region's nucleotide sequence