Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1049 and SO1050 are separated by 207 nucleotides SO1050 and SO1051 are separated by 119 nucleotides SO1051 and pit are separated by 188 nucleotides
SO1049: SO1049 - acetyltransferase, GNAT family (NCBI ptt file), at 1,086,745 to 1,087,221
SO1049
SO1050: SO1050 - hypothetical protein (NCBI ptt file), at 1,087,429 to 1,087,551
SO1050
SO1051: SO1051 - periplasmic glucan biosynthesis protein, putative (NCBI ptt file), at 1,087,671 to 1,089,464
SO1051
SO1052: pit - low-affinity inorganic phosphate transporter (NCBI ptt file), at 1,089,653 to 1,091,122
pit
Position (kb)
1087
1088
1089
1090 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1086.793 kb on - strand, within SO1049 at 1086.976 kb on - strand, within SO1049 at 1087.032 kb on - strand, within SO1049 at 1087.079 kb on + strand, within SO1049 at 1087.087 kb on - strand, within SO1049 at 1087.087 kb on - strand, within SO1049 at 1087.087 kb on - strand, within SO1049 at 1087.087 kb on - strand, within SO1049 at 1087.110 kb on + strand, within SO1049 at 1087.213 kb on - strand at 1087.213 kb on - strand at 1087.314 kb on - strand at 1087.398 kb on - strand at 1087.405 kb on + strand at 1087.427 kb on + strand at 1087.427 kb on + strand at 1087.435 kb on - strand at 1087.435 kb on - strand at 1087.469 kb on + strand, within SO1050 at 1087.606 kb on + strand at 1087.705 kb on - strand at 1087.705 kb on - strand at 1087.712 kb on + strand at 1087.818 kb on + strand at 1087.897 kb on + strand, within SO1051 at 1087.897 kb on + strand, within SO1051 at 1087.905 kb on - strand, within SO1051 at 1087.952 kb on + strand, within SO1051 at 1088.127 kb on + strand, within SO1051 at 1088.147 kb on - strand, within SO1051 at 1088.223 kb on - strand, within SO1051 at 1088.261 kb on + strand, within SO1051 at 1088.267 kb on + strand, within SO1051 at 1088.321 kb on + strand, within SO1051 at 1088.329 kb on - strand, within SO1051 at 1088.449 kb on - strand, within SO1051 at 1088.526 kb on + strand, within SO1051 at 1088.526 kb on + strand, within SO1051 at 1088.680 kb on - strand, within SO1051 at 1088.694 kb on + strand, within SO1051 at 1088.694 kb on + strand, within SO1051 at 1088.755 kb on - strand, within SO1051 at 1088.905 kb on - strand, within SO1051 at 1088.907 kb on - strand, within SO1051 at 1088.907 kb on - strand, within SO1051 at 1089.038 kb on - strand, within SO1051 at 1089.039 kb on + strand, within SO1051 at 1089.047 kb on - strand, within SO1051 at 1089.049 kb on + strand, within SO1051 at 1089.071 kb on + strand, within SO1051 at 1089.086 kb on + strand, within SO1051 at 1089.206 kb on + strand, within SO1051 at 1089.229 kb on - strand, within SO1051 at 1089.251 kb on - strand, within SO1051 at 1089.295 kb on - strand at 1089.314 kb on - strand at 1089.334 kb on + strand at 1089.334 kb on + strand at 1089.334 kb on + strand at 1089.334 kb on + strand at 1089.334 kb on + strand at 1089.337 kb on - strand at 1089.337 kb on - strand at 1089.337 kb on - strand at 1089.342 kb on - strand at 1089.349 kb on + strand at 1089.388 kb on - strand at 1089.440 kb on + strand at 1089.440 kb on + strand at 1089.499 kb on - strand at 1089.499 kb on - strand at 1089.560 kb on + strand at 1089.634 kb on + strand at 1089.685 kb on - strand at 1089.696 kb on + strand at 1089.696 kb on + strand at 1089.703 kb on - strand at 1089.757 kb on + strand at 1089.791 kb on - strand at 1089.867 kb on - strand, within pit at 1089.937 kb on - strand, within pit at 1090.027 kb on - strand, within pit at 1090.089 kb on + strand, within pit at 1090.089 kb on + strand, within pit at 1090.089 kb on + strand, within pit at 1090.089 kb on + strand, within pit at 1090.089 kb on + strand, within pit at 1090.089 kb on + strand, within pit at 1090.089 kb on + strand, within pit at 1090.089 kb on + strand, within pit at 1090.089 kb on + strand, within pit at 1090.097 kb on - strand, within pit at 1090.097 kb on - strand, within pit at 1090.099 kb on + strand, within pit at 1090.107 kb on - strand, within pit at 1090.151 kb on - strand, within pit at 1090.209 kb on - strand, within pit at 1090.305 kb on + strand, within pit at 1090.401 kb on - strand, within pit
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 1,086,793 - SO1049 0.10 -0.7 1,086,976 - SO1049 0.48 -0.4 1,087,032 - SO1049 0.60 +0.1 1,087,079 + SO1049 0.70 -0.6 1,087,087 - SO1049 0.72 -0.0 1,087,087 - SO1049 0.72 -0.6 1,087,087 - SO1049 0.72 +0.5 1,087,087 - SO1049 0.72 -0.2 1,087,110 + SO1049 0.77 +0.3 1,087,213 - +0.4 1,087,213 - +0.8 1,087,314 - -1.1 1,087,398 - -0.2 1,087,405 + +0.2 1,087,427 + -0.4 1,087,427 + +0.6 1,087,435 - -0.7 1,087,435 - +1.0 1,087,469 + SO1050 0.33 +0.4 1,087,606 + +0.7 1,087,705 - -0.7 1,087,705 - -1.3 1,087,712 + -0.6 1,087,818 + -1.1 1,087,897 + SO1051 0.13 -1.7 1,087,897 + SO1051 0.13 -1.2 1,087,905 - SO1051 0.13 -2.0 1,087,952 + SO1051 0.16 -1.8 1,088,127 + SO1051 0.25 -1.3 1,088,147 - SO1051 0.27 -1.1 1,088,223 - SO1051 0.31 -1.9 1,088,261 + SO1051 0.33 -2.3 1,088,267 + SO1051 0.33 -1.7 1,088,321 + SO1051 0.36 -1.8 1,088,329 - SO1051 0.37 -2.0 1,088,449 - SO1051 0.43 -1.3 1,088,526 + SO1051 0.48 -1.2 1,088,526 + SO1051 0.48 -0.0 1,088,680 - SO1051 0.56 -0.8 1,088,694 + SO1051 0.57 -0.8 1,088,694 + SO1051 0.57 -1.2 1,088,755 - SO1051 0.60 +0.4 1,088,905 - SO1051 0.69 -1.5 1,088,907 - SO1051 0.69 +0.4 1,088,907 - SO1051 0.69 -1.2 1,089,038 - SO1051 0.76 -0.4 1,089,039 + SO1051 0.76 -1.2 1,089,047 - SO1051 0.77 -2.0 1,089,049 + SO1051 0.77 -1.6 1,089,071 + SO1051 0.78 -1.3 1,089,086 + SO1051 0.79 -1.8 1,089,206 + SO1051 0.86 -2.5 1,089,229 - SO1051 0.87 -0.8 1,089,251 - SO1051 0.88 -1.8 1,089,295 - -1.1 1,089,314 - +0.3 1,089,334 + -1.4 1,089,334 + -1.9 1,089,334 + -1.4 1,089,334 + -1.3 1,089,334 + -1.8 1,089,337 - -1.4 1,089,337 - -1.9 1,089,337 - -1.0 1,089,342 - -2.7 1,089,349 + -1.2 1,089,388 - +0.3 1,089,440 + -1.6 1,089,440 + -1.8 1,089,499 - -0.3 1,089,499 - -0.3 1,089,560 + +0.1 1,089,634 + +0.3 1,089,685 - +1.2 1,089,696 + +0.2 1,089,696 + -1.3 1,089,703 - +0.5 1,089,757 + +1.1 1,089,791 - +1.0 1,089,867 - pit SO1052 0.15 -0.1 1,089,937 - pit SO1052 0.19 +0.7 1,090,027 - pit SO1052 0.25 +1.5 1,090,089 + pit SO1052 0.30 +1.0 1,090,089 + pit SO1052 0.30 +0.3 1,090,089 + pit SO1052 0.30 +0.5 1,090,089 + pit SO1052 0.30 +0.9 1,090,089 + pit SO1052 0.30 +0.3 1,090,089 + pit SO1052 0.30 +0.2 1,090,089 + pit SO1052 0.30 +0.8 1,090,089 + pit SO1052 0.30 -0.2 1,090,089 + pit SO1052 0.30 +1.9 1,090,097 - pit SO1052 0.30 -0.1 1,090,097 - pit SO1052 0.30 +0.8 1,090,099 + pit SO1052 0.30 -0.0 1,090,107 - pit SO1052 0.31 +0.7 1,090,151 - pit SO1052 0.34 +1.0 1,090,209 - pit SO1052 0.38 -0.2 1,090,305 + pit SO1052 0.44 +0.8 1,090,401 - pit SO1052 0.51 +0.7
Or see this region's nucleotide sequence