Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoL and nuoK overlap by 4 nucleotides nuoK and nuoJ overlap by 4 nucleotides nuoJ and nuoI are separated by 10 nucleotides nuoI and nuoH are separated by 8 nucleotides nuoH and nuoG overlap by 4 nucleotides
SO1011: nuoL - NADH dehydrogenase I, L subunit (NCBI ptt file), at 1,048,384 to 1,050,231
nuoL
SO1012: nuoK - NADH dehydrogenase I, K subunit (NCBI ptt file), at 1,050,228 to 1,050,536
nuoK
SO1013: nuoJ - NADH dehydrogenase I, J subunit (NCBI ptt file), at 1,050,533 to 1,051,084
nuoJ
SO1014: nuoI - NADH dehydrogenase I, I subunit (NCBI ptt file), at 1,051,095 to 1,051,637
nuoI
SO1015: nuoH - NADH dehydrogenase I, H subunit (NCBI ptt file), at 1,051,646 to 1,052,614
nuoH
SO1016: nuoG - NADH dehydrogenase I, G subunit (NCBI ptt file), at 1,052,611 to 1,055,340
nuoG
Position (kb)
1051
1052 Strain fitness (log2 ratio)
-2
-1
0
1 at 1050.114 kb on + strand at 1050.114 kb on + strand at 1050.122 kb on - strand at 1050.133 kb on + strand at 1050.178 kb on - strand at 1050.183 kb on - strand at 1050.261 kb on - strand, within nuoK at 1050.285 kb on - strand, within nuoK at 1050.315 kb on - strand, within nuoK at 1050.371 kb on - strand, within nuoK at 1050.459 kb on + strand, within nuoK at 1050.467 kb on - strand, within nuoK at 1050.481 kb on + strand, within nuoK at 1050.481 kb on + strand, within nuoK at 1050.503 kb on + strand, within nuoK at 1050.503 kb on + strand, within nuoK at 1050.511 kb on - strand at 1050.543 kb on + strand at 1050.587 kb on - strand at 1050.589 kb on + strand, within nuoJ at 1050.605 kb on - strand, within nuoJ at 1050.696 kb on + strand, within nuoJ at 1050.706 kb on + strand, within nuoJ at 1050.706 kb on + strand, within nuoJ at 1050.714 kb on - strand, within nuoJ at 1051.006 kb on + strand, within nuoJ at 1051.155 kb on - strand, within nuoI at 1051.318 kb on + strand, within nuoI at 1051.446 kb on - strand, within nuoI at 1051.454 kb on - strand, within nuoI at 1051.485 kb on + strand, within nuoI at 1051.485 kb on + strand, within nuoI at 1051.493 kb on - strand, within nuoI at 1051.611 kb on + strand at 1051.677 kb on + strand at 1051.735 kb on - strand at 1051.746 kb on - strand, within nuoH at 1051.786 kb on + strand, within nuoH at 1051.786 kb on + strand, within nuoH at 1051.815 kb on - strand, within nuoH at 1051.869 kb on - strand, within nuoH at 1051.974 kb on + strand, within nuoH at 1051.996 kb on + strand, within nuoH at 1052.063 kb on + strand, within nuoH at 1052.063 kb on + strand, within nuoH at 1052.073 kb on + strand, within nuoH at 1052.165 kb on + strand, within nuoH at 1052.165 kb on + strand, within nuoH at 1052.165 kb on + strand, within nuoH at 1052.261 kb on + strand, within nuoH at 1052.268 kb on + strand, within nuoH at 1052.268 kb on + strand, within nuoH at 1052.403 kb on + strand, within nuoH
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 1,050,114 + -1.7 1,050,114 + -0.8 1,050,122 - -2.7 1,050,133 + -1.5 1,050,178 - -2.2 1,050,183 - -0.8 1,050,261 - nuoK SO1012 0.11 -0.7 1,050,285 - nuoK SO1012 0.18 -1.3 1,050,315 - nuoK SO1012 0.28 -1.5 1,050,371 - nuoK SO1012 0.46 +0.0 1,050,459 + nuoK SO1012 0.75 -2.1 1,050,467 - nuoK SO1012 0.77 -0.3 1,050,481 + nuoK SO1012 0.82 -1.9 1,050,481 + nuoK SO1012 0.82 +0.2 1,050,503 + nuoK SO1012 0.89 -1.1 1,050,503 + nuoK SO1012 0.89 -1.2 1,050,511 - -2.2 1,050,543 + -1.5 1,050,587 - -1.0 1,050,589 + nuoJ SO1013 0.10 -1.1 1,050,605 - nuoJ SO1013 0.13 -1.1 1,050,696 + nuoJ SO1013 0.30 -2.6 1,050,706 + nuoJ SO1013 0.31 -2.6 1,050,706 + nuoJ SO1013 0.31 -2.0 1,050,714 - nuoJ SO1013 0.33 -1.7 1,051,006 + nuoJ SO1013 0.86 -0.9 1,051,155 - nuoI SO1014 0.11 -2.2 1,051,318 + nuoI SO1014 0.41 -1.7 1,051,446 - nuoI SO1014 0.65 -1.0 1,051,454 - nuoI SO1014 0.66 -0.6 1,051,485 + nuoI SO1014 0.72 -1.8 1,051,485 + nuoI SO1014 0.72 -1.4 1,051,493 - nuoI SO1014 0.73 -1.4 1,051,611 + -1.7 1,051,677 + -1.2 1,051,735 - -0.8 1,051,746 - nuoH SO1015 0.10 -2.5 1,051,786 + nuoH SO1015 0.14 -1.4 1,051,786 + nuoH SO1015 0.14 -1.1 1,051,815 - nuoH SO1015 0.17 -0.9 1,051,869 - nuoH SO1015 0.23 -2.5 1,051,974 + nuoH SO1015 0.34 -0.9 1,051,996 + nuoH SO1015 0.36 -0.9 1,052,063 + nuoH SO1015 0.43 -1.1 1,052,063 + nuoH SO1015 0.43 -1.6 1,052,073 + nuoH SO1015 0.44 -1.9 1,052,165 + nuoH SO1015 0.54 -1.8 1,052,165 + nuoH SO1015 0.54 -2.0 1,052,165 + nuoH SO1015 0.54 -2.4 1,052,261 + nuoH SO1015 0.63 -0.3 1,052,268 + nuoH SO1015 0.64 -2.3 1,052,268 + nuoH SO1015 0.64 -0.6 1,052,403 + nuoH SO1015 0.78 -1.7
Or see this region's nucleotide sequence