Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0621 and SO0622 overlap by 4 nucleotides SO0622 and SO0623 overlap by 20 nucleotides SO0623 and crp are separated by 82 nucleotides
SO0621: SO0621 - sensor histidine kinase (NCBI ptt file), at 648,335 to 649,663
SO0621
SO0622: SO0622 - DNA-binding response regulator (NCBI ptt file), at 649,660 to 650,334
SO0622
SO0623: SO0623 - hypothetical protein (NCBI ptt file), at 650,315 to 650,662
SO0623
SO0624: crp - catabolite gene activator (NCBI ptt file), at 650,745 to 651,380
crp
Position (kb) 650
651 Strain fitness (log2 ratio) -2
-1
0
1
2 at 649.411 kb on - strand, within SO0621 at 649.526 kb on + strand, within SO0621 at 649.564 kb on - strand at 649.607 kb on + strand at 649.607 kb on + strand at 649.689 kb on - strand at 649.753 kb on - strand, within SO0622 at 649.857 kb on + strand, within SO0622 at 649.857 kb on + strand, within SO0622 at 649.928 kb on - strand, within SO0622 at 650.065 kb on + strand, within SO0622 at 650.065 kb on + strand, within SO0622 at 650.109 kb on + strand, within SO0622 at 650.299 kb on - strand at 650.301 kb on + strand at 650.366 kb on - strand, within SO0623 at 650.376 kb on - strand, within SO0623 at 650.455 kb on - strand, within SO0623 at 650.520 kb on - strand, within SO0623 at 650.520 kb on - strand, within SO0623 at 650.540 kb on - strand, within SO0623 at 650.555 kb on - strand, within SO0623 at 650.555 kb on - strand, within SO0623 at 650.583 kb on - strand, within SO0623 at 650.619 kb on + strand, within SO0623 at 650.620 kb on + strand, within SO0623 at 650.720 kb on + strand at 650.823 kb on + strand, within crp at 650.892 kb on + strand, within crp at 650.984 kb on + strand, within crp at 650.992 kb on - strand, within crp at 650.994 kb on + strand, within crp at 651.105 kb on - strand, within crp at 651.112 kb on + strand, within crp at 651.119 kb on + strand, within crp at 651.120 kb on - strand, within crp at 651.125 kb on - strand, within crp at 651.127 kb on - strand, within crp at 651.152 kb on + strand, within crp at 651.192 kb on - strand, within crp at 651.192 kb on - strand, within crp at 651.225 kb on - strand, within crp at 651.254 kb on + strand, within crp at 651.254 kb on + strand, within crp at 651.254 kb on + strand, within crp at 651.262 kb on - strand, within crp at 651.270 kb on + strand, within crp at 651.270 kb on + strand, within crp at 651.270 kb on + strand, within crp at 651.270 kb on + strand, within crp at 651.278 kb on - strand, within crp at 651.278 kb on - strand, within crp at 651.278 kb on - strand, within crp at 651.280 kb on + strand, within crp at 651.285 kb on + strand, within crp at 651.285 kb on + strand, within crp at 651.292 kb on - strand, within crp at 651.355 kb on + strand at 651.369 kb on + strand at 651.369 kb on + strand at 651.377 kb on - strand at 651.471 kb on - strand at 651.520 kb on - strand at 651.559 kb on + strand at 651.567 kb on - strand at 651.567 kb on - strand at 651.579 kb on + strand at 651.587 kb on + strand at 651.587 kb on + strand at 651.587 kb on + strand at 651.587 kb on + strand at 651.587 kb on + strand at 651.587 kb on + strand at 651.587 kb on - strand at 651.587 kb on - strand at 651.587 kb on - strand at 651.587 kb on - strand at 651.592 kb on + strand at 651.592 kb on - strand at 651.595 kb on - strand at 651.595 kb on - strand at 651.595 kb on - strand at 651.595 kb on - strand at 651.595 kb on - strand at 651.595 kb on - strand at 651.595 kb on - strand at 651.595 kb on - strand at 651.597 kb on + strand at 651.605 kb on - strand at 651.623 kb on + strand at 651.650 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 649,411 - SO0621 0.81 -1.4 649,526 + SO0621 0.90 -1.8 649,564 - -2.2 649,607 + -2.6 649,607 + -0.2 649,689 - +0.4 649,753 - SO0622 0.14 +0.3 649,857 + SO0622 0.29 +0.5 649,857 + SO0622 0.29 -0.2 649,928 - SO0622 0.40 +0.2 650,065 + SO0622 0.60 +0.1 650,065 + SO0622 0.60 +0.1 650,109 + SO0622 0.67 -0.2 650,299 - +2.0 650,301 + +0.0 650,366 - SO0623 0.15 -1.6 650,376 - SO0623 0.18 -0.8 650,455 - SO0623 0.40 -0.5 650,520 - SO0623 0.59 +1.3 650,520 - SO0623 0.59 +0.9 650,540 - SO0623 0.65 +0.6 650,555 - SO0623 0.69 -0.1 650,555 - SO0623 0.69 +0.2 650,583 - SO0623 0.77 +2.3 650,619 + SO0623 0.87 +0.7 650,620 + SO0623 0.88 -0.3 650,720 + +1.7 650,823 + crp SO0624 0.12 +1.6 650,892 + crp SO0624 0.23 +2.1 650,984 + crp SO0624 0.38 +2.1 650,992 - crp SO0624 0.39 +2.2 650,994 + crp SO0624 0.39 +1.6 651,105 - crp SO0624 0.57 +1.3 651,112 + crp SO0624 0.58 +1.9 651,119 + crp SO0624 0.59 +1.2 651,120 - crp SO0624 0.59 +2.4 651,125 - crp SO0624 0.60 +2.2 651,127 - crp SO0624 0.60 +1.9 651,152 + crp SO0624 0.64 +1.9 651,192 - crp SO0624 0.70 +1.5 651,192 - crp SO0624 0.70 +1.2 651,225 - crp SO0624 0.75 +1.2 651,254 + crp SO0624 0.80 +1.9 651,254 + crp SO0624 0.80 +1.8 651,254 + crp SO0624 0.80 +2.2 651,262 - crp SO0624 0.81 +1.5 651,270 + crp SO0624 0.83 +1.8 651,270 + crp SO0624 0.83 +0.6 651,270 + crp SO0624 0.83 +1.9 651,270 + crp SO0624 0.83 +1.6 651,278 - crp SO0624 0.84 +1.9 651,278 - crp SO0624 0.84 +1.7 651,278 - crp SO0624 0.84 +1.5 651,280 + crp SO0624 0.84 +1.4 651,285 + crp SO0624 0.85 +2.2 651,285 + crp SO0624 0.85 +2.0 651,292 - crp SO0624 0.86 +2.3 651,355 + +2.4 651,369 + +1.5 651,369 + +1.7 651,377 - +2.7 651,471 - -0.1 651,520 - +0.6 651,559 + +0.6 651,567 - -0.3 651,567 - -0.6 651,579 + -0.1 651,587 + -0.4 651,587 + -0.7 651,587 + -0.3 651,587 + +0.2 651,587 + +0.1 651,587 + -0.6 651,587 - +0.6 651,587 - -0.2 651,587 - -1.0 651,587 - +1.1 651,592 + -0.6 651,592 - +0.3 651,595 - +1.1 651,595 - +0.8 651,595 - +0.8 651,595 - -1.3 651,595 - -2.3 651,595 - +0.4 651,595 - +0.6 651,595 - +1.9 651,597 + -0.2 651,605 - +0.8 651,623 + +0.0 651,650 + -1.3
Or see this region's nucleotide sequence