Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt selD and SO0197 are separated by 96 nucleotides SO0197 and SO0198 are separated by 143 nucleotides
SO0196: selD - selenide, water dikinase (NCBI ptt file), at 206,086 to 207,144
selD
SO0197: SO0197 - fatty acid desaturase, family 1 (NCBI ptt file), at 207,241 to 208,347
SO0197
SO0198: SO0198 - transcriptional regulator, TetR family (NCBI ptt file), at 208,491 to 209,105
SO0198
Position (kb) 207
208
209 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 206.336 kb on - strand, within selD at 206.391 kb on + strand, within selD at 206.399 kb on - strand, within selD at 206.403 kb on + strand, within selD at 206.431 kb on + strand, within selD at 206.438 kb on - strand, within selD at 206.439 kb on - strand, within selD at 206.439 kb on - strand, within selD at 206.465 kb on + strand, within selD at 206.465 kb on + strand, within selD at 206.465 kb on + strand, within selD at 206.666 kb on + strand, within selD at 206.674 kb on - strand, within selD at 206.674 kb on - strand, within selD at 206.674 kb on - strand, within selD at 206.674 kb on - strand, within selD at 206.674 kb on - strand, within selD at 206.743 kb on + strand, within selD at 206.751 kb on - strand, within selD at 206.962 kb on + strand, within selD at 206.966 kb on - strand, within selD at 207.113 kb on - strand at 207.126 kb on + strand at 207.189 kb on + strand at 207.244 kb on + strand at 207.361 kb on - strand, within SO0197 at 207.367 kb on + strand, within SO0197 at 207.367 kb on + strand, within SO0197 at 207.375 kb on - strand, within SO0197 at 207.379 kb on + strand, within SO0197 at 207.656 kb on + strand, within SO0197 at 207.656 kb on + strand, within SO0197 at 207.667 kb on + strand, within SO0197 at 207.675 kb on - strand, within SO0197 at 207.675 kb on - strand, within SO0197 at 207.713 kb on + strand, within SO0197 at 207.721 kb on - strand, within SO0197 at 207.721 kb on - strand, within SO0197 at 207.736 kb on - strand, within SO0197 at 207.761 kb on + strand, within SO0197 at 207.767 kb on - strand, within SO0197 at 207.843 kb on - strand, within SO0197 at 207.850 kb on - strand, within SO0197 at 207.868 kb on - strand, within SO0197 at 207.876 kb on + strand, within SO0197 at 207.876 kb on + strand, within SO0197 at 207.933 kb on + strand, within SO0197 at 207.941 kb on - strand, within SO0197 at 207.947 kb on + strand, within SO0197 at 207.955 kb on - strand, within SO0197 at 207.961 kb on - strand, within SO0197 at 208.016 kb on + strand, within SO0197 at 208.024 kb on - strand, within SO0197 at 208.090 kb on + strand, within SO0197 at 208.147 kb on + strand, within SO0197 at 208.170 kb on + strand, within SO0197 at 208.177 kb on - strand, within SO0197 at 208.210 kb on + strand, within SO0197 at 208.210 kb on + strand, within SO0197 at 208.210 kb on + strand, within SO0197 at 208.210 kb on + strand, within SO0197 at 208.210 kb on + strand, within SO0197 at 208.218 kb on - strand, within SO0197 at 208.218 kb on - strand, within SO0197 at 208.218 kb on - strand, within SO0197 at 208.250 kb on + strand at 208.252 kb on - strand at 208.299 kb on - strand at 208.299 kb on - strand at 208.309 kb on - strand at 208.317 kb on + strand at 208.317 kb on + strand at 208.325 kb on - strand at 208.422 kb on + strand at 208.426 kb on - strand at 208.435 kb on - strand at 208.556 kb on + strand, within SO0198 at 208.556 kb on + strand, within SO0198 at 208.556 kb on + strand, within SO0198 at 208.556 kb on + strand, within SO0198 at 208.556 kb on + strand, within SO0198 at 208.561 kb on + strand, within SO0198 at 208.564 kb on - strand, within SO0198 at 208.564 kb on - strand, within SO0198 at 208.564 kb on - strand, within SO0198 at 208.564 kb on - strand, within SO0198 at 208.564 kb on - strand, within SO0198 at 208.564 kb on - strand, within SO0198 at 208.564 kb on - strand, within SO0198 at 208.564 kb on - strand, within SO0198 at 208.564 kb on - strand, within SO0198 at 208.712 kb on + strand, within SO0198 at 208.737 kb on - strand, within SO0198 at 208.939 kb on + strand, within SO0198 at 208.945 kb on - strand, within SO0198 at 208.947 kb on - strand, within SO0198 at 209.006 kb on - strand, within SO0198 at 209.022 kb on - strand, within SO0198 at 209.099 kb on - strand at 209.163 kb on + strand at 209.195 kb on + strand at 209.253 kb on + strand at 209.261 kb on - strand at 209.261 kb on - strand at 209.281 kb on + strand at 209.281 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 206,336 - selD SO0196 0.24 +1.4 206,391 + selD SO0196 0.29 -0.1 206,399 - selD SO0196 0.30 +0.3 206,403 + selD SO0196 0.30 +0.9 206,431 + selD SO0196 0.33 +0.4 206,438 - selD SO0196 0.33 +0.8 206,439 - selD SO0196 0.33 -0.5 206,439 - selD SO0196 0.33 +0.6 206,465 + selD SO0196 0.36 +0.4 206,465 + selD SO0196 0.36 +0.2 206,465 + selD SO0196 0.36 +0.9 206,666 + selD SO0196 0.55 +1.3 206,674 - selD SO0196 0.56 +0.3 206,674 - selD SO0196 0.56 +0.7 206,674 - selD SO0196 0.56 +0.7 206,674 - selD SO0196 0.56 +0.1 206,674 - selD SO0196 0.56 +0.2 206,743 + selD SO0196 0.62 -1.2 206,751 - selD SO0196 0.63 -0.1 206,962 + selD SO0196 0.83 -0.2 206,966 - selD SO0196 0.83 +0.5 207,113 - +0.3 207,126 + +0.2 207,189 + -0.2 207,244 + +1.0 207,361 - SO0197 0.11 +0.9 207,367 + SO0197 0.11 -0.6 207,367 + SO0197 0.11 +2.7 207,375 - SO0197 0.12 -0.0 207,379 + SO0197 0.12 +0.9 207,656 + SO0197 0.37 +0.5 207,656 + SO0197 0.37 -1.2 207,667 + SO0197 0.38 +1.2 207,675 - SO0197 0.39 +1.1 207,675 - SO0197 0.39 -0.3 207,713 + SO0197 0.43 -0.1 207,721 - SO0197 0.43 +1.4 207,721 - SO0197 0.43 -0.5 207,736 - SO0197 0.45 +0.6 207,761 + SO0197 0.47 +0.9 207,767 - SO0197 0.48 +1.0 207,843 - SO0197 0.54 -0.4 207,850 - SO0197 0.55 +0.9 207,868 - SO0197 0.57 -1.3 207,876 + SO0197 0.57 +0.4 207,876 + SO0197 0.57 +0.3 207,933 + SO0197 0.63 +0.4 207,941 - SO0197 0.63 -1.1 207,947 + SO0197 0.64 +1.2 207,955 - SO0197 0.64 +1.5 207,961 - SO0197 0.65 +0.5 208,016 + SO0197 0.70 +0.1 208,024 - SO0197 0.71 +0.6 208,090 + SO0197 0.77 +0.2 208,147 + SO0197 0.82 +0.1 208,170 + SO0197 0.84 -0.3 208,177 - SO0197 0.85 -0.8 208,210 + SO0197 0.88 +0.4 208,210 + SO0197 0.88 -0.8 208,210 + SO0197 0.88 +1.1 208,210 + SO0197 0.88 +0.5 208,210 + SO0197 0.88 -2.2 208,218 - SO0197 0.88 +0.8 208,218 - SO0197 0.88 -0.8 208,218 - SO0197 0.88 +0.1 208,250 + -1.0 208,252 - +1.3 208,299 - +0.5 208,299 - +1.6 208,309 - +0.5 208,317 + +1.2 208,317 + +0.5 208,325 - +0.9 208,422 + +0.9 208,426 - +0.2 208,435 - -0.1 208,556 + SO0198 0.11 -1.6 208,556 + SO0198 0.11 -2.3 208,556 + SO0198 0.11 -1.8 208,556 + SO0198 0.11 -1.6 208,556 + SO0198 0.11 -1.7 208,561 + SO0198 0.11 -4.4 208,564 - SO0198 0.12 -2.8 208,564 - SO0198 0.12 -1.9 208,564 - SO0198 0.12 -3.1 208,564 - SO0198 0.12 -1.8 208,564 - SO0198 0.12 -3.2 208,564 - SO0198 0.12 -2.3 208,564 - SO0198 0.12 -1.3 208,564 - SO0198 0.12 -2.6 208,564 - SO0198 0.12 -2.3 208,712 + SO0198 0.36 -2.7 208,737 - SO0198 0.40 -1.7 208,939 + SO0198 0.73 -2.6 208,945 - SO0198 0.74 -2.0 208,947 - SO0198 0.74 +0.4 209,006 - SO0198 0.84 -1.2 209,022 - SO0198 0.86 -0.5 209,099 - -0.3 209,163 + +0.1 209,195 + +0.7 209,253 + -1.3 209,261 - +0.0 209,261 - +0.4 209,281 + -0.5 209,281 + +0.8
Or see this region's nucleotide sequence