Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoE and rseA are separated by 42 nucleotides rseA and rseB are separated by 12 nucleotides rseB and rseC are separated by 11 nucleotides rseC and lepA are separated by 167 nucleotides
SO1342: rpoE - RNA polymerase sigma-24 factor (NCBI ptt file), at 1,399,196 to 1,399,774
rpoE
SO1343: rseA - sigma-E factor negative regulatory protein (NCBI ptt file), at 1,399,817 to 1,400,440
rseA
SO1344: rseB - sigma-E factor regulatory protein RseB (NCBI ptt file), at 1,400,453 to 1,401,385
rseB
SO1345: rseC - sigma-E factor regulatory protein RseC (NCBI ptt file), at 1,401,397 to 1,401,870
rseC
SO1346: lepA - GTP-binding protein LepA (NCBI ptt file), at 1,402,038 to 1,403,828
lepA
Position (kb)
1400
1401
1402 Strain fitness (log2 ratio)
-2
-1
0
1 at 1400.093 kb on + strand, within rseA at 1400.437 kb on + strand at 1400.437 kb on + strand at 1400.673 kb on - strand, within rseB at 1400.712 kb on + strand, within rseB at 1400.720 kb on - strand, within rseB at 1400.920 kb on + strand, within rseB at 1401.235 kb on + strand, within rseB at 1401.278 kb on + strand, within rseB at 1401.935 kb on + strand at 1401.994 kb on + strand at 1402.134 kb on - strand at 1402.382 kb on + strand, within lepA
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,400,093 + rseA SO1343 0.44 +0.2 1,400,437 + -1.0 1,400,437 + +0.8 1,400,673 - rseB SO1344 0.24 -1.3 1,400,712 + rseB SO1344 0.28 -0.8 1,400,720 - rseB SO1344 0.29 -1.1 1,400,920 + rseB SO1344 0.50 +0.5 1,401,235 + rseB SO1344 0.84 -1.3 1,401,278 + rseB SO1344 0.88 -0.1 1,401,935 + -0.2 1,401,994 + -0.9 1,402,134 - -2.4 1,402,382 + lepA SO1346 0.19 -0.1
Or see this region's nucleotide sequence