Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nqrB-2 and nqrC-2 overlap by 8 nucleotides nqrC-2 and nqrD-2 overlap by 1 nucleotides nqrD-2 and nqrE-2 are separated by 5 nucleotides
SO1104: nqrB-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrB (NCBI ptt file), at 1,147,570 to 1,148,769
nqrB-2
SO1105: nqrC-2 - NADH:ubiquinone oxidoreductase, Na translocating, gamma subunit (NCBI ptt file), at 1,148,762 to 1,149,550
nqrC-2
SO1106: nqrD-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrD (NCBI ptt file), at 1,149,550 to 1,150,182
nqrD-2
SO1107: nqrE-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrE (NCBI ptt file), at 1,150,188 to 1,150,796
nqrE-2
Position (kb)
1148
1149
1150 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1147.766 kb on - strand, within nqrB-2 at 1147.981 kb on + strand, within nqrB-2 at 1148.277 kb on - strand, within nqrB-2 at 1148.282 kb on + strand, within nqrB-2 at 1148.429 kb on + strand, within nqrB-2 at 1148.736 kb on + strand at 1149.336 kb on - strand, within nqrC-2 at 1149.408 kb on - strand, within nqrC-2 at 1149.774 kb on - strand, within nqrD-2 at 1150.157 kb on - strand at 1150.323 kb on + strand, within nqrE-2 at 1150.427 kb on - strand, within nqrE-2 at 1150.539 kb on + strand, within nqrE-2
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,147,766 - nqrB-2 SO1104 0.16 -1.3 1,147,981 + nqrB-2 SO1104 0.34 -1.0 1,148,277 - nqrB-2 SO1104 0.59 -0.6 1,148,282 + nqrB-2 SO1104 0.59 -1.8 1,148,429 + nqrB-2 SO1104 0.72 -0.8 1,148,736 + +3.4 1,149,336 - nqrC-2 SO1105 0.73 -1.4 1,149,408 - nqrC-2 SO1105 0.82 -1.1 1,149,774 - nqrD-2 SO1106 0.35 -1.0 1,150,157 - -2.6 1,150,323 + nqrE-2 SO1107 0.22 -1.0 1,150,427 - nqrE-2 SO1107 0.39 -2.5 1,150,539 + nqrE-2 SO1107 0.58 -2.7
Or see this region's nucleotide sequence