Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt brnQ and xerD are separated by 80 nucleotides xerD and dsbC are separated by 150 nucleotides dsbC and recJ are separated by 92 nucleotides
SO0949: brnQ - branched-chain amino acid transport system II carrier protein BrnQ (NCBI ptt file), at 985,012 to 986,400
brnQ
SO0950: xerD - integrase/recombinase XerD (NCBI ptt file), at 986,481 to 987,383
xerD
SO0951: dsbC - thiol:disulfide interchange protein DsbC (NCBI ptt file), at 987,534 to 988,259
dsbC
SO0952: recJ - single-stranded-DNA-specific exonuclease RecJ (NCBI ptt file), at 988,352 to 990,076
recJ
Position (kb) 986
987
988 Strain fitness (log2 ratio) -2
-1
0
1
2 at 985.619 kb on + strand, within brnQ at 985.627 kb on + strand, within brnQ at 985.627 kb on + strand, within brnQ at 985.627 kb on + strand, within brnQ at 985.727 kb on - strand, within brnQ at 985.730 kb on + strand, within brnQ at 985.912 kb on + strand, within brnQ at 986.149 kb on - strand, within brnQ at 986.184 kb on + strand, within brnQ at 986.205 kb on + strand, within brnQ at 986.333 kb on + strand at 986.333 kb on + strand at 986.341 kb on - strand at 986.341 kb on - strand at 986.341 kb on - strand at 986.341 kb on - strand at 986.400 kb on + strand at 986.541 kb on + strand at 986.555 kb on - strand at 986.561 kb on + strand at 986.614 kb on + strand, within xerD at 986.622 kb on - strand, within xerD at 986.622 kb on - strand, within xerD at 986.704 kb on - strand, within xerD at 986.704 kb on - strand, within xerD at 986.762 kb on + strand, within xerD at 986.794 kb on + strand, within xerD at 986.818 kb on - strand, within xerD at 986.841 kb on - strand, within xerD at 986.861 kb on + strand, within xerD at 986.875 kb on + strand, within xerD at 986.880 kb on + strand, within xerD at 986.880 kb on + strand, within xerD at 986.883 kb on - strand, within xerD at 986.883 kb on - strand, within xerD at 986.920 kb on + strand, within xerD at 986.925 kb on + strand, within xerD at 986.973 kb on - strand, within xerD at 986.983 kb on + strand, within xerD at 987.013 kb on - strand, within xerD at 987.049 kb on - strand, within xerD at 987.227 kb on - strand, within xerD at 987.284 kb on - strand, within xerD at 987.312 kb on + strand at 987.360 kb on - strand at 987.377 kb on + strand at 987.385 kb on - strand at 987.482 kb on + strand at 987.482 kb on + strand at 987.482 kb on + strand at 987.482 kb on + strand at 987.482 kb on + strand at 987.482 kb on + strand at 987.490 kb on - strand at 987.490 kb on - strand at 987.490 kb on - strand at 987.490 kb on - strand at 987.490 kb on - strand at 987.494 kb on + strand at 987.590 kb on - strand at 987.590 kb on - strand at 987.595 kb on - strand at 987.595 kb on - strand at 987.609 kb on + strand, within dsbC at 987.725 kb on + strand, within dsbC at 987.779 kb on + strand, within dsbC at 987.833 kb on - strand, within dsbC at 987.880 kb on - strand, within dsbC at 987.924 kb on - strand, within dsbC at 987.990 kb on + strand, within dsbC at 988.000 kb on - strand, within dsbC at 988.050 kb on + strand, within dsbC at 988.087 kb on - strand, within dsbC at 988.115 kb on - strand, within dsbC at 988.240 kb on + strand at 988.240 kb on + strand at 988.240 kb on + strand at 988.240 kb on + strand at 988.248 kb on - strand at 988.248 kb on - strand at 988.248 kb on - strand at 988.248 kb on - strand at 988.248 kb on - strand at 988.248 kb on - strand at 988.295 kb on + strand at 988.303 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 985,619 + brnQ SO0949 0.44 +0.3 985,627 + brnQ SO0949 0.44 -0.3 985,627 + brnQ SO0949 0.44 -0.0 985,627 + brnQ SO0949 0.44 +0.2 985,727 - brnQ SO0949 0.51 -0.5 985,730 + brnQ SO0949 0.52 -1.5 985,912 + brnQ SO0949 0.65 +0.4 986,149 - brnQ SO0949 0.82 +0.1 986,184 + brnQ SO0949 0.84 -0.6 986,205 + brnQ SO0949 0.86 +0.7 986,333 + +0.8 986,333 + -0.1 986,341 - -0.4 986,341 - -0.7 986,341 - +0.3 986,341 - -0.1 986,400 + -0.2 986,541 + -1.3 986,555 - -1.9 986,561 + -2.0 986,614 + xerD SO0950 0.15 -1.6 986,622 - xerD SO0950 0.16 -1.5 986,622 - xerD SO0950 0.16 -2.2 986,704 - xerD SO0950 0.25 -1.3 986,704 - xerD SO0950 0.25 -1.7 986,762 + xerD SO0950 0.31 -1.9 986,794 + xerD SO0950 0.35 -0.6 986,818 - xerD SO0950 0.37 -1.5 986,841 - xerD SO0950 0.40 -2.0 986,861 + xerD SO0950 0.42 -0.4 986,875 + xerD SO0950 0.44 -0.7 986,880 + xerD SO0950 0.44 -0.7 986,880 + xerD SO0950 0.44 -1.8 986,883 - xerD SO0950 0.45 -2.0 986,883 - xerD SO0950 0.45 -1.3 986,920 + xerD SO0950 0.49 -2.2 986,925 + xerD SO0950 0.49 -0.8 986,973 - xerD SO0950 0.54 -0.7 986,983 + xerD SO0950 0.56 -1.5 987,013 - xerD SO0950 0.59 -1.1 987,049 - xerD SO0950 0.63 -1.2 987,227 - xerD SO0950 0.83 -1.2 987,284 - xerD SO0950 0.89 -0.9 987,312 + -1.9 987,360 - -1.5 987,377 + +0.5 987,385 - +0.2 987,482 + -0.6 987,482 + -0.3 987,482 + +2.0 987,482 + +0.5 987,482 + -0.2 987,482 + -0.3 987,490 - -0.2 987,490 - +0.1 987,490 - +0.1 987,490 - +0.6 987,490 - +0.2 987,494 + -0.4 987,590 - +0.2 987,590 - -0.5 987,595 - +0.7 987,595 - -0.2 987,609 + dsbC SO0951 0.10 +0.1 987,725 + dsbC SO0951 0.26 -1.5 987,779 + dsbC SO0951 0.34 -0.3 987,833 - dsbC SO0951 0.41 -0.1 987,880 - dsbC SO0951 0.48 -0.3 987,924 - dsbC SO0951 0.54 +0.1 987,990 + dsbC SO0951 0.63 +0.3 988,000 - dsbC SO0951 0.64 -0.5 988,050 + dsbC SO0951 0.71 +0.2 988,087 - dsbC SO0951 0.76 -0.2 988,115 - dsbC SO0951 0.80 -0.7 988,240 + +0.3 988,240 + -0.2 988,240 + +0.4 988,240 + -0.3 988,248 - -0.2 988,248 - -0.5 988,248 - +0.6 988,248 - -0.3 988,248 - +0.9 988,248 - +0.6 988,295 + -1.3 988,303 - +0.1
Or see this region's nucleotide sequence