Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt panB and folK-1 are separated by 56 nucleotides folK-1 and pcnB overlap by 1 nucleotides pcnB and SO0873 are separated by 274 nucleotides
SO0870: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI ptt file), at 900,169 to 900,963
panB
SO0871: folK-1 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (NCBI ptt file), at 901,020 to 901,508
folK-1
SO0872: pcnB - polyA polymerase (NCBI ptt file), at 901,508 to 902,824
pcnB
SO0873: SO0873 - glutamyl-tRNA synthetase domain protein (NCBI ptt file), at 903,099 to 903,998
SO0873
Position (kb) 901
902
903 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 900.551 kb on - strand, within panB at 900.740 kb on - strand, within panB at 900.800 kb on - strand, within panB at 900.947 kb on - strand at 900.962 kb on - strand at 901.040 kb on - strand at 901.074 kb on + strand, within folK-1 at 901.571 kb on - strand at 901.752 kb on - strand, within pcnB at 902.032 kb on - strand, within pcnB at 902.288 kb on - strand, within pcnB at 902.288 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.358 kb on - strand, within pcnB at 902.714 kb on - strand at 902.744 kb on - strand at 902.750 kb on + strand at 902.833 kb on - strand at 902.868 kb on - strand at 902.896 kb on - strand at 903.055 kb on - strand at 903.055 kb on - strand at 903.134 kb on - strand at 903.207 kb on + strand, within SO0873 at 903.216 kb on + strand, within SO0873 at 903.227 kb on + strand, within SO0873 at 903.438 kb on - strand, within SO0873 at 903.467 kb on + strand, within SO0873 at 903.591 kb on + strand, within SO0873 at 903.721 kb on + strand, within SO0873 at 903.751 kb on + strand, within SO0873 at 903.768 kb on + strand, within SO0873 at 903.784 kb on - strand, within SO0873
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 900,551 - panB SO0870 0.48 -0.1 900,740 - panB SO0870 0.72 -2.9 900,800 - panB SO0870 0.79 +0.5 900,947 - +0.0 900,962 - -0.4 901,040 - +0.0 901,074 + folK-1 SO0871 0.11 +0.0 901,571 - -1.2 901,752 - pcnB SO0872 0.19 -0.5 902,032 - pcnB SO0872 0.40 -1.2 902,288 - pcnB SO0872 0.59 -1.0 902,288 - pcnB SO0872 0.59 -0.2 902,343 - pcnB SO0872 0.63 -0.6 902,343 - pcnB SO0872 0.63 -0.8 902,343 - pcnB SO0872 0.63 -0.3 902,343 - pcnB SO0872 0.63 -0.5 902,343 - pcnB SO0872 0.63 +0.1 902,343 - pcnB SO0872 0.63 -0.4 902,343 - pcnB SO0872 0.63 -0.8 902,343 - pcnB SO0872 0.63 -1.2 902,343 - pcnB SO0872 0.63 -1.8 902,343 - pcnB SO0872 0.63 -0.2 902,358 - pcnB SO0872 0.65 -0.9 902,714 - +0.3 902,744 - +0.3 902,750 + -0.6 902,833 - -1.4 902,868 - -0.6 902,896 - -1.7 903,055 - -0.7 903,055 - +0.6 903,134 - +0.1 903,207 + SO0873 0.12 -0.2 903,216 + SO0873 0.13 +0.3 903,227 + SO0873 0.14 +0.3 903,438 - SO0873 0.38 -0.6 903,467 + SO0873 0.41 -0.6 903,591 + SO0873 0.55 +0.1 903,721 + SO0873 0.69 -0.2 903,751 + SO0873 0.72 +0.4 903,768 + SO0873 0.74 +0.4 903,784 - SO0873 0.76 +0.5
Or see this region's nucleotide sequence