Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hflK and hflC are separated by 3 nucleotides hflC and petA are separated by 360 nucleotides petA and petB overlap by 1 nucleotides
SO0605: hflK - hflK protein (NCBI ptt file), at 631,396 to 632,541
hflK
SO0606: hflC - hflC protein (NCBI ptt file), at 632,545 to 633,438
hflC
SO0608: petA - ubiquinol-cytochrome c reductase, iron-sulfur subunit (NCBI ptt file), at 633,799 to 634,389
petA
SO0609: petB - ubiquinol-cytochrome c reductase, cytochrome b (NCBI ptt file), at 634,389 to 635,603
petB
Position (kb) 632
633
634 Strain fitness (log2 ratio) -2
-1
0
1 at 631.550 kb on + strand, within hflK at 631.550 kb on + strand, within hflK at 632.028 kb on + strand, within hflK at 632.119 kb on + strand, within hflK at 632.247 kb on + strand, within hflK at 632.254 kb on + strand, within hflK at 632.259 kb on + strand, within hflK at 632.316 kb on + strand, within hflK at 632.406 kb on + strand, within hflK at 632.430 kb on + strand at 632.430 kb on + strand at 632.430 kb on + strand at 632.590 kb on + strand at 632.744 kb on - strand, within hflC at 632.765 kb on + strand, within hflC at 632.903 kb on + strand, within hflC at 633.052 kb on + strand, within hflC at 633.231 kb on + strand, within hflC at 633.332 kb on + strand, within hflC at 633.845 kb on - strand at 634.379 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 631,550 + hflK SO0605 0.13 +0.5 631,550 + hflK SO0605 0.13 -0.4 632,028 + hflK SO0605 0.55 -0.4 632,119 + hflK SO0605 0.63 -0.7 632,247 + hflK SO0605 0.74 -0.2 632,254 + hflK SO0605 0.75 -0.3 632,259 + hflK SO0605 0.75 +0.2 632,316 + hflK SO0605 0.80 +0.8 632,406 + hflK SO0605 0.88 -0.1 632,430 + +0.3 632,430 + +0.0 632,430 + +0.0 632,590 + -1.8 632,744 - hflC SO0606 0.22 -1.4 632,765 + hflC SO0606 0.25 -0.8 632,903 + hflC SO0606 0.40 +0.2 633,052 + hflC SO0606 0.57 -0.4 633,231 + hflC SO0606 0.77 -0.9 633,332 + hflC SO0606 0.88 -0.7 633,845 - -1.5 634,379 - -1.4
Or see this region's nucleotide sequence