Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ccmE and ccmD overlap by 4 nucleotides ccmD and ccmC overlap by 1 nucleotides ccmC and ccmB are separated by 1 nucleotides ccmB and ccmA are separated by 11 nucleotides ccmA and scyA are separated by 325 nucleotides
SO0259: ccmE - cytochrome c biogenesis protein CcmE (NCBI ptt file), at 260,541 to 261,026
ccmE
SO0260: ccmD - heme exporter protein CcmD (NCBI ptt file), at 261,023 to 261,223
ccmD
SO0261: ccmC - heme exporter protein CcmC (NCBI ptt file), at 261,223 to 261,969
ccmC
SO0262: ccmB - heme exporter protein CcmB (NCBI ptt file), at 261,971 to 262,657
ccmB
SO0263: ccmA - heme exporter protein CcmA (NCBI ptt file), at 262,669 to 263,319
ccmA
SO0264: scyA - cytochrome c (NCBI ptt file), at 263,645 to 263,944
scyA
Position (kb) 261
262
263 Strain fitness (log2 ratio) -2
-1
0
1 at 261.000 kb on - strand at 261.581 kb on - strand, within ccmC at 261.600 kb on + strand, within ccmC at 261.638 kb on + strand, within ccmC at 261.801 kb on - strand, within ccmC at 261.952 kb on - strand at 262.012 kb on + strand at 262.226 kb on + strand, within ccmB at 262.372 kb on + strand, within ccmB at 262.377 kb on + strand, within ccmB at 262.401 kb on - strand, within ccmB at 262.641 kb on - strand at 262.666 kb on + strand at 262.668 kb on - strand at 262.876 kb on - strand, within ccmA at 262.899 kb on - strand, within ccmA at 263.041 kb on - strand, within ccmA at 263.120 kb on - strand, within ccmA at 263.120 kb on - strand, within ccmA at 263.267 kb on + strand at 263.290 kb on - strand at 263.382 kb on + strand at 263.397 kb on + strand at 263.406 kb on - strand at 263.406 kb on - strand at 263.406 kb on - strand at 263.430 kb on - strand at 263.431 kb on - strand at 263.499 kb on + strand at 263.509 kb on + strand at 263.582 kb on + strand at 263.587 kb on - strand at 263.593 kb on + strand at 263.613 kb on - strand at 263.636 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 261,000 - +0.5 261,581 - ccmC SO0261 0.48 +0.1 261,600 + ccmC SO0261 0.50 -1.5 261,638 + ccmC SO0261 0.56 -0.6 261,801 - ccmC SO0261 0.77 -2.4 261,952 - -0.7 262,012 + -0.3 262,226 + ccmB SO0262 0.37 -0.8 262,372 + ccmB SO0262 0.58 -2.4 262,377 + ccmB SO0262 0.59 +0.0 262,401 - ccmB SO0262 0.63 -1.1 262,641 - -0.8 262,666 + -0.6 262,668 - -0.6 262,876 - ccmA SO0263 0.32 -1.0 262,899 - ccmA SO0263 0.35 -0.9 263,041 - ccmA SO0263 0.57 -0.9 263,120 - ccmA SO0263 0.69 +0.5 263,120 - ccmA SO0263 0.69 -1.3 263,267 + -0.8 263,290 - -0.2 263,382 + -0.9 263,397 + +0.3 263,406 - +0.1 263,406 - +0.3 263,406 - -0.3 263,430 - +0.6 263,431 - -0.2 263,499 + +0.1 263,509 + +0.0 263,582 + +0.9 263,587 - +0.3 263,593 + -0.6 263,613 - -0.7 263,636 + +0.3
Or see this region's nucleotide sequence