Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspK and gspL are separated by 48 nucleotides gspL and gspM are separated by 1 nucleotides gspM and gspN are separated by 14 nucleotides
SO0173: gspK - general secretion pathway protein K (NCBI ptt file), at 179,387 to 180,385
gspK
SO0174: gspL - general secretion pathway protein L (NCBI ptt file), at 180,434 to 181,621
gspL
SO0175: gspM - general secretion pathway protein M (NCBI ptt file), at 181,623 to 182,099
gspM
SO0176: gspN - general secretion pathway protein N (NCBI ptt file), at 182,114 to 182,875
gspN
Position (kb) 180
181
182 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 179.436 kb on - strand at 179.544 kb on - strand, within gspK at 179.635 kb on + strand, within gspK at 179.645 kb on + strand, within gspK at 179.785 kb on + strand, within gspK at 179.849 kb on - strand, within gspK at 179.892 kb on + strand, within gspK at 180.055 kb on + strand, within gspK at 180.102 kb on - strand, within gspK at 180.209 kb on - strand, within gspK at 180.410 kb on + strand at 180.418 kb on - strand at 180.424 kb on + strand at 180.476 kb on - strand at 180.493 kb on + strand at 180.538 kb on + strand at 180.546 kb on - strand at 180.546 kb on - strand at 180.569 kb on - strand, within gspL at 180.569 kb on - strand, within gspL at 180.569 kb on - strand, within gspL at 180.628 kb on - strand, within gspL at 180.628 kb on - strand, within gspL at 180.697 kb on - strand, within gspL at 180.910 kb on - strand, within gspL at 180.920 kb on + strand, within gspL at 180.932 kb on + strand, within gspL at 181.035 kb on + strand, within gspL at 181.078 kb on - strand, within gspL at 181.147 kb on - strand, within gspL at 181.225 kb on - strand, within gspL at 181.281 kb on - strand, within gspL at 181.311 kb on - strand, within gspL at 181.364 kb on + strand, within gspL at 181.364 kb on + strand, within gspL at 181.372 kb on - strand, within gspL at 181.372 kb on - strand, within gspL at 181.404 kb on - strand, within gspL at 181.416 kb on + strand, within gspL at 181.591 kb on - strand at 181.604 kb on + strand at 181.612 kb on - strand at 181.651 kb on + strand at 181.659 kb on - strand at 181.659 kb on - strand at 181.659 kb on - strand at 181.734 kb on + strand, within gspM at 181.754 kb on - strand, within gspM at 181.798 kb on + strand, within gspM at 181.881 kb on - strand, within gspM at 182.067 kb on + strand at 182.081 kb on + strand at 182.104 kb on + strand at 182.149 kb on + strand at 182.149 kb on + strand at 182.152 kb on - strand at 182.171 kb on + strand at 182.229 kb on + strand, within gspN at 182.319 kb on + strand, within gspN at 182.404 kb on - strand, within gspN at 182.420 kb on + strand, within gspN at 182.425 kb on - strand, within gspN at 182.548 kb on + strand, within gspN
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 179,436 - -2.0 179,544 - gspK SO0173 0.16 -2.9 179,635 + gspK SO0173 0.25 -1.4 179,645 + gspK SO0173 0.26 -0.9 179,785 + gspK SO0173 0.40 -2.4 179,849 - gspK SO0173 0.46 -1.5 179,892 + gspK SO0173 0.51 -0.9 180,055 + gspK SO0173 0.67 -0.7 180,102 - gspK SO0173 0.72 -1.5 180,209 - gspK SO0173 0.82 -1.4 180,410 + +0.4 180,418 - -0.5 180,424 + +0.1 180,476 - -1.8 180,493 + -0.9 180,538 + -1.5 180,546 - -2.3 180,546 - -0.1 180,569 - gspL SO0174 0.11 -1.2 180,569 - gspL SO0174 0.11 -1.7 180,569 - gspL SO0174 0.11 -0.1 180,628 - gspL SO0174 0.16 -0.6 180,628 - gspL SO0174 0.16 -2.4 180,697 - gspL SO0174 0.22 -2.3 180,910 - gspL SO0174 0.40 -2.1 180,920 + gspL SO0174 0.41 -1.4 180,932 + gspL SO0174 0.42 -2.4 181,035 + gspL SO0174 0.51 -1.6 181,078 - gspL SO0174 0.54 -3.0 181,147 - gspL SO0174 0.60 -2.3 181,225 - gspL SO0174 0.67 -2.3 181,281 - gspL SO0174 0.71 -1.1 181,311 - gspL SO0174 0.74 -0.6 181,364 + gspL SO0174 0.78 -1.2 181,364 + gspL SO0174 0.78 -1.4 181,372 - gspL SO0174 0.79 -0.8 181,372 - gspL SO0174 0.79 -1.3 181,404 - gspL SO0174 0.82 -3.0 181,416 + gspL SO0174 0.83 -2.4 181,591 - -1.5 181,604 + -0.0 181,612 - -1.4 181,651 + -0.8 181,659 - -1.1 181,659 - -0.9 181,659 - -1.6 181,734 + gspM SO0175 0.23 -0.4 181,754 - gspM SO0175 0.27 -2.0 181,798 + gspM SO0175 0.37 -2.1 181,881 - gspM SO0175 0.54 -2.4 182,067 + -1.0 182,081 + +0.1 182,104 + +0.1 182,149 + -0.7 182,149 + -1.9 182,152 - -0.5 182,171 + -1.7 182,229 + gspN SO0176 0.15 -0.7 182,319 + gspN SO0176 0.27 -1.7 182,404 - gspN SO0176 0.38 -1.4 182,420 + gspN SO0176 0.40 +0.6 182,425 - gspN SO0176 0.41 -1.8 182,548 + gspN SO0176 0.57 -2.4
Or see this region's nucleotide sequence