Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspF and gspG are separated by 74 nucleotides gspG and gspH are separated by 1 nucleotides gspH and gspI overlap by 14 nucleotides gspI and gspJ overlap by 20 nucleotides
SO0168: gspF - general secretion pathway protein F (NCBI ptt file), at 175,981 to 177,204
gspF
SO0169: gspG - general secretion pathway protein G (NCBI ptt file), at 177,279 to 177,713
gspG
SO0170: gspH - general secretion pathway protein H (NCBI ptt file), at 177,715 to 178,287
gspH
SO0171: gspI - general secretion pathway protein I (NCBI ptt file), at 178,274 to 178,642
gspI
SO0172: gspJ - general secretion pathway protein J (NCBI ptt file), at 178,623 to 179,390
gspJ
Position (kb) 177
178
179 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 176.795 kb on - strand, within gspF at 176.834 kb on + strand, within gspF at 176.834 kb on + strand, within gspF at 176.936 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.953 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.966 kb on - strand, within gspF at 176.966 kb on - strand, within gspF at 177.010 kb on - strand, within gspF at 177.118 kb on + strand at 177.269 kb on + strand at 177.277 kb on - strand at 177.303 kb on + strand at 177.303 kb on + strand at 177.329 kb on + strand, within gspG at 177.364 kb on - strand, within gspG at 177.366 kb on - strand, within gspG at 177.397 kb on - strand, within gspG at 177.476 kb on + strand, within gspG at 177.492 kb on + strand, within gspG at 177.554 kb on + strand, within gspG at 177.613 kb on - strand, within gspG at 177.638 kb on + strand, within gspG at 177.662 kb on + strand, within gspG at 177.751 kb on - strand at 177.788 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.845 kb on - strand, within gspH at 177.845 kb on - strand, within gspH at 178.065 kb on - strand, within gspH at 178.221 kb on - strand, within gspH at 178.237 kb on + strand at 178.266 kb on - strand at 178.282 kb on + strand at 178.440 kb on + strand, within gspI at 178.516 kb on - strand, within gspI at 178.605 kb on - strand, within gspI at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.662 kb on - strand at 178.662 kb on - strand at 178.700 kb on + strand, within gspJ at 178.730 kb on - strand, within gspJ at 178.742 kb on - strand, within gspJ at 178.744 kb on + strand, within gspJ at 178.816 kb on + strand, within gspJ at 178.824 kb on - strand, within gspJ at 178.849 kb on - strand, within gspJ at 178.850 kb on + strand, within gspJ at 178.867 kb on + strand, within gspJ at 178.943 kb on + strand, within gspJ at 178.943 kb on + strand, within gspJ at 178.950 kb on - strand, within gspJ at 178.951 kb on - strand, within gspJ at 179.018 kb on - strand, within gspJ at 179.151 kb on + strand, within gspJ at 179.157 kb on + strand, within gspJ at 179.220 kb on - strand, within gspJ at 179.273 kb on + strand, within gspJ
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 176,795 - gspF SO0168 0.67 -2.3 176,834 + gspF SO0168 0.70 -1.3 176,834 + gspF SO0168 0.70 -1.7 176,936 + gspF SO0168 0.78 -1.5 176,941 + gspF SO0168 0.78 -1.7 176,941 + gspF SO0168 0.78 -2.2 176,941 + gspF SO0168 0.78 -0.1 176,953 + gspF SO0168 0.79 -0.5 176,958 + gspF SO0168 0.80 -1.0 176,958 + gspF SO0168 0.80 -1.5 176,966 - gspF SO0168 0.80 -2.4 176,966 - gspF SO0168 0.80 -2.8 177,010 - gspF SO0168 0.84 -0.1 177,118 + -0.5 177,269 + +0.2 177,277 - -1.2 177,303 + -2.8 177,303 + -1.0 177,329 + gspG SO0169 0.11 -2.2 177,364 - gspG SO0169 0.20 -2.4 177,366 - gspG SO0169 0.20 -1.3 177,397 - gspG SO0169 0.27 -1.1 177,476 + gspG SO0169 0.45 -1.7 177,492 + gspG SO0169 0.49 -2.1 177,554 + gspG SO0169 0.63 -2.2 177,613 - gspG SO0169 0.77 -0.9 177,638 + gspG SO0169 0.83 -3.0 177,662 + gspG SO0169 0.88 -1.4 177,751 - -1.4 177,788 + gspH SO0170 0.13 -0.9 177,837 + gspH SO0170 0.21 -3.3 177,837 + gspH SO0170 0.21 -0.2 177,837 + gspH SO0170 0.21 -0.5 177,845 - gspH SO0170 0.23 -2.2 177,845 - gspH SO0170 0.23 -1.9 178,065 - gspH SO0170 0.61 -2.4 178,221 - gspH SO0170 0.88 -1.7 178,237 + -0.1 178,266 - -1.1 178,282 + -0.5 178,440 + gspI SO0171 0.45 -1.6 178,516 - gspI SO0171 0.66 -2.6 178,605 - gspI SO0171 0.90 -0.6 178,654 + -1.0 178,654 + -0.4 178,654 + -2.1 178,654 + -1.9 178,654 + -2.6 178,654 + -2.4 178,662 - -1.3 178,662 - -1.6 178,700 + gspJ SO0172 0.10 -1.0 178,730 - gspJ SO0172 0.14 -2.1 178,742 - gspJ SO0172 0.15 -4.0 178,744 + gspJ SO0172 0.16 -0.1 178,816 + gspJ SO0172 0.25 -0.6 178,824 - gspJ SO0172 0.26 -2.1 178,849 - gspJ SO0172 0.29 -0.9 178,850 + gspJ SO0172 0.30 -1.8 178,867 + gspJ SO0172 0.32 -1.4 178,943 + gspJ SO0172 0.42 -1.0 178,943 + gspJ SO0172 0.42 -0.3 178,950 - gspJ SO0172 0.43 -0.0 178,951 - gspJ SO0172 0.43 -0.7 179,018 - gspJ SO0172 0.51 -2.5 179,151 + gspJ SO0172 0.69 -2.5 179,157 + gspJ SO0172 0.70 -0.1 179,220 - gspJ SO0172 0.78 -0.9 179,273 + gspJ SO0172 0.85 -0.8
Or see this region's nucleotide sequence