Strain Fitness in Shewanella oneidensis MR-1 around SO0010

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdnaN and recF are separated by 162 nucleotidesrecF and gyrB are separated by 16 nucleotides SO0009: dnaN - DNA polymerase III, beta subunit (NCBI ptt file), at 8,276 to 9,376 dnaN SO0010: recF - DNA replication and repair protein RecF (NCBI ptt file), at 9,539 to 10,621 recF SO0011: gyrB - DNA gyrase, B subunit (NCBI ptt file), at 10,638 to 13,055 gyrB Position (kb) 9 10 11Strain fitness (log2 ratio) -2 -1 0 1at 9.363 kb on - strandat 9.368 kb on + strandat 9.418 kb on + strandat 9.418 kb on + strandat 9.426 kb on - strandat 9.426 kb on - strandat 9.427 kb on + strandat 9.507 kb on - strandat 9.507 kb on - strandat 9.507 kb on - strandat 9.507 kb on - strandat 9.543 kb on + strandat 9.543 kb on + strandat 9.551 kb on - strandat 9.551 kb on - strandat 9.617 kb on - strandat 9.617 kb on - strandat 9.746 kb on - strand, within recFat 9.756 kb on + strand, within recFat 9.764 kb on - strand, within recFat 9.842 kb on + strand, within recFat 9.863 kb on + strand, within recFat 9.966 kb on - strand, within recFat 10.144 kb on + strand, within recFat 10.144 kb on + strand, within recFat 10.144 kb on + strand, within recFat 10.177 kb on + strand, within recFat 10.259 kb on + strand, within recFat 10.291 kb on + strand, within recF

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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9,363 - +0.1
9,368 + -0.3
9,418 + +0.6
9,418 + +0.4
9,426 - +0.5
9,426 - +0.3
9,427 + +0.9
9,507 - -0.1
9,507 - -0.5
9,507 - -2.4
9,507 - -0.6
9,543 + -1.1
9,543 + -1.3
9,551 - -0.8
9,551 - -0.3
9,617 - +0.6
9,617 - +0.1
9,746 - recF SO0010 0.19 -1.2
9,756 + recF SO0010 0.20 -0.0
9,764 - recF SO0010 0.21 -0.4
9,842 + recF SO0010 0.28 -0.8
9,863 + recF SO0010 0.30 -0.7
9,966 - recF SO0010 0.39 -2.1
10,144 + recF SO0010 0.56 -1.1
10,144 + recF SO0010 0.56 -0.7
10,144 + recF SO0010 0.56 -0.6
10,177 + recF SO0010 0.59 -1.0
10,259 + recF SO0010 0.66 -1.4
10,291 + recF SO0010 0.69 -1.9

Or see this region's nucleotide sequence