Experiment: L-Lysine 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3282 and cydB are separated by 445 nucleotides cydB and cydA are separated by 15 nucleotides
SO3282: SO3282 - methyl-accepting chemotaxis protein (NCBI ptt file), at 3,422,537 to 3,424,540
SO3282
SO3285: cydB - cytochrome d ubiquinol oxidase, subunit II (NCBI ptt file), at 3,424,986 to 3,426,125
cydB
SO3286: cydA - cytochrome d ubiquinol oxidase, subunit I (NCBI ptt file), at 3,426,141 to 3,427,697
cydA
Position (kb)
3424
3425
3426
3427 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3424.053 kb on + strand, within SO3282 at 3424.150 kb on + strand, within SO3282 at 3424.171 kb on + strand, within SO3282 at 3424.234 kb on - strand, within SO3282 at 3424.445 kb on + strand at 3424.603 kb on + strand at 3424.636 kb on + strand at 3424.978 kb on + strand at 3424.982 kb on + strand at 3424.986 kb on - strand at 3425.000 kb on - strand at 3425.067 kb on + strand at 3425.067 kb on + strand at 3425.072 kb on + strand at 3425.075 kb on - strand at 3425.075 kb on - strand at 3425.167 kb on - strand, within cydB at 3425.211 kb on + strand, within cydB at 3425.299 kb on + strand, within cydB at 3425.398 kb on + strand, within cydB at 3425.398 kb on + strand, within cydB at 3425.401 kb on - strand, within cydB at 3425.406 kb on - strand, within cydB at 3425.406 kb on - strand, within cydB at 3425.406 kb on - strand, within cydB at 3425.406 kb on - strand, within cydB at 3425.548 kb on - strand, within cydB at 3425.852 kb on + strand, within cydB at 3425.887 kb on + strand, within cydB at 3426.081 kb on + strand at 3426.081 kb on + strand at 3426.089 kb on - strand at 3426.089 kb on - strand at 3426.239 kb on + strand at 3426.244 kb on + strand at 3426.244 kb on + strand at 3426.244 kb on + strand at 3426.244 kb on + strand at 3426.252 kb on - strand at 3426.252 kb on - strand at 3426.252 kb on - strand at 3426.252 kb on - strand at 3426.252 kb on - strand at 3426.252 kb on - strand at 3426.316 kb on - strand, within cydA at 3426.334 kb on - strand, within cydA at 3426.334 kb on - strand, within cydA at 3426.334 kb on - strand, within cydA at 3426.334 kb on - strand, within cydA at 3426.351 kb on + strand, within cydA at 3426.403 kb on + strand, within cydA at 3426.531 kb on + strand, within cydA at 3426.539 kb on - strand, within cydA at 3426.610 kb on + strand, within cydA at 3426.909 kb on + strand, within cydA at 3426.986 kb on - strand, within cydA
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 100 mM remove 3,424,053 + SO3282 0.76 -0.5 3,424,150 + SO3282 0.80 +0.1 3,424,171 + SO3282 0.82 -0.3 3,424,234 - SO3282 0.85 -0.1 3,424,445 + -0.4 3,424,603 + -0.1 3,424,636 + -0.0 3,424,978 + -1.9 3,424,982 + -1.1 3,424,986 - -1.1 3,425,000 - -2.2 3,425,067 + -1.4 3,425,067 + -0.5 3,425,072 + -0.7 3,425,075 - -1.2 3,425,075 - -1.6 3,425,167 - cydB SO3285 0.16 -1.8 3,425,211 + cydB SO3285 0.20 -1.0 3,425,299 + cydB SO3285 0.27 -2.1 3,425,398 + cydB SO3285 0.36 -3.1 3,425,398 + cydB SO3285 0.36 -1.8 3,425,401 - cydB SO3285 0.36 -1.0 3,425,406 - cydB SO3285 0.37 -3.0 3,425,406 - cydB SO3285 0.37 -0.6 3,425,406 - cydB SO3285 0.37 -0.9 3,425,406 - cydB SO3285 0.37 -0.8 3,425,548 - cydB SO3285 0.49 -1.5 3,425,852 + cydB SO3285 0.76 -2.5 3,425,887 + cydB SO3285 0.79 -2.7 3,426,081 + -0.9 3,426,081 + -0.5 3,426,089 - -0.4 3,426,089 - -1.6 3,426,239 + -1.1 3,426,244 + +0.4 3,426,244 + -2.6 3,426,244 + -2.3 3,426,244 + -1.9 3,426,252 - -1.2 3,426,252 - -3.1 3,426,252 - -1.1 3,426,252 - -2.6 3,426,252 - -1.7 3,426,252 - -1.6 3,426,316 - cydA SO3286 0.11 -2.8 3,426,334 - cydA SO3286 0.12 -0.5 3,426,334 - cydA SO3286 0.12 -1.8 3,426,334 - cydA SO3286 0.12 -1.4 3,426,334 - cydA SO3286 0.12 -1.6 3,426,351 + cydA SO3286 0.13 -0.5 3,426,403 + cydA SO3286 0.17 -0.1 3,426,531 + cydA SO3286 0.25 -0.8 3,426,539 - cydA SO3286 0.26 -0.1 3,426,610 + cydA SO3286 0.30 -2.1 3,426,909 + cydA SO3286 0.49 -1.5 3,426,986 - cydA SO3286 0.54 -0.9
Or see this region's nucleotide sequence