Experiment: L-Lysine 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepE and mdoG-2 are separated by 652 nucleotides mdoG-2 and SO2732 are separated by 181 nucleotides
SO2730: pepE - peptidase E (NCBI ptt file), at 2,845,799 to 2,846,509
pepE
SO2731: mdoG-2 - periplasmic glucans biosynthesis protein MdoG (NCBI ptt file), at 2,847,162 to 2,848,748
mdoG-2
SO2732: SO2732 - ISSod6, transposase (NCBI ptt file), at 2,848,930 to 2,849,688
SO2732
Position (kb)
2847
2848
2849 Strain fitness (log2 ratio)
-2
-1
0
1 at 2846.169 kb on - strand, within pepE at 2846.225 kb on - strand, within pepE at 2846.240 kb on + strand, within pepE at 2846.248 kb on - strand, within pepE at 2846.375 kb on + strand, within pepE at 2846.383 kb on - strand, within pepE at 2846.383 kb on - strand, within pepE at 2846.393 kb on - strand, within pepE at 2846.401 kb on + strand, within pepE at 2846.409 kb on - strand, within pepE at 2846.477 kb on - strand at 2846.515 kb on + strand at 2846.523 kb on + strand at 2846.523 kb on - strand at 2846.940 kb on + strand at 2846.940 kb on + strand at 2846.940 kb on + strand at 2846.948 kb on - strand at 2846.948 kb on - strand at 2847.018 kb on - strand at 2847.178 kb on - strand at 2847.238 kb on + strand at 2847.279 kb on + strand at 2847.324 kb on + strand, within mdoG-2 at 2847.395 kb on - strand, within mdoG-2 at 2847.418 kb on - strand, within mdoG-2 at 2847.557 kb on - strand, within mdoG-2 at 2847.620 kb on + strand, within mdoG-2 at 2847.660 kb on - strand, within mdoG-2 at 2847.744 kb on + strand, within mdoG-2 at 2847.752 kb on - strand, within mdoG-2 at 2847.794 kb on + strand, within mdoG-2 at 2847.794 kb on + strand, within mdoG-2 at 2847.802 kb on - strand, within mdoG-2 at 2847.802 kb on - strand, within mdoG-2 at 2847.802 kb on - strand, within mdoG-2 at 2847.807 kb on + strand, within mdoG-2 at 2847.842 kb on + strand, within mdoG-2 at 2848.029 kb on + strand, within mdoG-2 at 2848.065 kb on + strand, within mdoG-2 at 2848.076 kb on - strand, within mdoG-2 at 2848.078 kb on - strand, within mdoG-2 at 2848.100 kb on + strand, within mdoG-2 at 2848.100 kb on + strand, within mdoG-2 at 2848.181 kb on + strand, within mdoG-2 at 2848.362 kb on + strand, within mdoG-2 at 2848.370 kb on - strand, within mdoG-2 at 2848.372 kb on + strand, within mdoG-2 at 2848.390 kb on - strand, within mdoG-2 at 2848.465 kb on + strand, within mdoG-2 at 2848.571 kb on + strand, within mdoG-2 at 2848.572 kb on + strand, within mdoG-2 at 2848.618 kb on - strand at 2848.618 kb on - strand at 2848.745 kb on - strand at 2848.844 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 100 mM remove 2,846,169 - pepE SO2730 0.52 -0.1 2,846,225 - pepE SO2730 0.60 -0.2 2,846,240 + pepE SO2730 0.62 -0.2 2,846,248 - pepE SO2730 0.63 -0.2 2,846,375 + pepE SO2730 0.81 +1.0 2,846,383 - pepE SO2730 0.82 -0.5 2,846,383 - pepE SO2730 0.82 -0.2 2,846,393 - pepE SO2730 0.84 +0.2 2,846,401 + pepE SO2730 0.85 -0.5 2,846,409 - pepE SO2730 0.86 +0.2 2,846,477 - -0.5 2,846,515 + +0.1 2,846,523 + -0.9 2,846,523 - -0.1 2,846,940 + -0.2 2,846,940 + +0.4 2,846,940 + +0.2 2,846,948 - -0.1 2,846,948 - -1.2 2,847,018 - -1.0 2,847,178 - -1.5 2,847,238 + -0.6 2,847,279 + -1.6 2,847,324 + mdoG-2 SO2731 0.10 -0.1 2,847,395 - mdoG-2 SO2731 0.15 +0.2 2,847,418 - mdoG-2 SO2731 0.16 -0.9 2,847,557 - mdoG-2 SO2731 0.25 -0.5 2,847,620 + mdoG-2 SO2731 0.29 +0.2 2,847,660 - mdoG-2 SO2731 0.31 -1.2 2,847,744 + mdoG-2 SO2731 0.37 -1.2 2,847,752 - mdoG-2 SO2731 0.37 -1.4 2,847,794 + mdoG-2 SO2731 0.40 -0.5 2,847,794 + mdoG-2 SO2731 0.40 -0.4 2,847,802 - mdoG-2 SO2731 0.40 +0.4 2,847,802 - mdoG-2 SO2731 0.40 -0.8 2,847,802 - mdoG-2 SO2731 0.40 -2.3 2,847,807 + mdoG-2 SO2731 0.41 -0.7 2,847,842 + mdoG-2 SO2731 0.43 -0.7 2,848,029 + mdoG-2 SO2731 0.55 -1.0 2,848,065 + mdoG-2 SO2731 0.57 +0.1 2,848,076 - mdoG-2 SO2731 0.58 -0.8 2,848,078 - mdoG-2 SO2731 0.58 -1.0 2,848,100 + mdoG-2 SO2731 0.59 +1.0 2,848,100 + mdoG-2 SO2731 0.59 -1.1 2,848,181 + mdoG-2 SO2731 0.64 -1.4 2,848,362 + mdoG-2 SO2731 0.76 +0.2 2,848,370 - mdoG-2 SO2731 0.76 -0.3 2,848,372 + mdoG-2 SO2731 0.76 -1.0 2,848,390 - mdoG-2 SO2731 0.77 -0.6 2,848,465 + mdoG-2 SO2731 0.82 -1.2 2,848,571 + mdoG-2 SO2731 0.89 -1.2 2,848,572 + mdoG-2 SO2731 0.89 -2.4 2,848,618 - -1.1 2,848,618 - -1.0 2,848,745 - -0.6 2,848,844 - -0.2
Or see this region's nucleotide sequence