Experiment: L-Lysine 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rmf and fabA are separated by 108 nucleotides fabA and SO1857 are separated by 73 nucleotides SO1857 and tRNA-Ser-2 are separated by 221 nucleotides tRNA-Ser-2 and SO1858 are separated by 27 nucleotides
SO1855: rmf - ribosome modulation factor (NCBI ptt file), at 1,947,212 to 1,947,388
rmf
SO1856: fabA - 3-hydroxydecanoyl-(acyl-carrier-protein) dehydratase (NCBI ptt file), at 1,947,497 to 1,948,012
fabA
SO1857: SO1857 - hypothetical ATP-dependent protease (NCBI ptt file), at 1,948,086 to 1,949,819
SO1857
SO_t031: tRNA-Ser-2 - tRNA-Ser (RefSeq), at 1,950,041 to 1,950,131
tRNA-Ser-2
SO1858: SO1858 - hypothetical protein (NCBI ptt file), at 1,950,159 to 1,950,755
SO1858
Position (kb)
1948
1949
1950 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1947.174 kb on - strand at 1947.285 kb on + strand, within rmf at 1947.292 kb on + strand, within rmf at 1947.338 kb on - strand, within rmf at 1947.420 kb on - strand at 1947.426 kb on + strand at 1947.434 kb on - strand at 1947.454 kb on - strand at 1947.495 kb on + strand at 1947.712 kb on - strand, within fabA at 1947.972 kb on - strand at 1948.064 kb on - strand at 1948.178 kb on + strand at 1948.178 kb on + strand at 1948.186 kb on - strand at 1948.186 kb on - strand at 1948.293 kb on + strand, within SO1857 at 1948.293 kb on + strand, within SO1857 at 1948.306 kb on - strand, within SO1857 at 1948.383 kb on - strand, within SO1857 at 1948.508 kb on + strand, within SO1857 at 1948.579 kb on - strand, within SO1857 at 1948.579 kb on - strand, within SO1857 at 1948.624 kb on - strand, within SO1857 at 1948.645 kb on - strand, within SO1857 at 1948.759 kb on + strand, within SO1857 at 1948.773 kb on - strand, within SO1857 at 1948.773 kb on - strand, within SO1857 at 1948.793 kb on + strand, within SO1857 at 1948.809 kb on - strand, within SO1857 at 1948.927 kb on - strand, within SO1857 at 1948.968 kb on - strand, within SO1857 at 1949.077 kb on - strand, within SO1857 at 1949.077 kb on - strand, within SO1857 at 1949.278 kb on - strand, within SO1857 at 1949.328 kb on + strand, within SO1857 at 1949.364 kb on + strand, within SO1857 at 1949.444 kb on + strand, within SO1857 at 1949.514 kb on + strand, within SO1857 at 1949.744 kb on - strand at 1949.771 kb on + strand at 1949.832 kb on + strand at 1949.933 kb on - strand at 1949.980 kb on - strand at 1950.604 kb on - strand, within SO1858 at 1950.663 kb on + strand, within SO1858 at 1950.668 kb on + strand, within SO1858 at 1950.676 kb on - strand, within SO1858 at 1950.695 kb on + strand, within SO1858 at 1950.782 kb on - strand at 1950.784 kb on + strand at 1950.784 kb on + strand at 1950.792 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 100 mM remove 1,947,174 - -0.1 1,947,285 + rmf SO1855 0.41 -0.5 1,947,292 + rmf SO1855 0.45 +0.2 1,947,338 - rmf SO1855 0.71 +0.1 1,947,420 - -0.4 1,947,426 + +0.5 1,947,434 - -1.1 1,947,454 - +0.1 1,947,495 + +0.4 1,947,712 - fabA SO1856 0.42 +0.2 1,947,972 - +0.8 1,948,064 - +0.5 1,948,178 + -0.5 1,948,178 + -0.3 1,948,186 - +1.0 1,948,186 - +0.0 1,948,293 + SO1857 0.12 +1.0 1,948,293 + SO1857 0.12 +0.1 1,948,306 - SO1857 0.13 -1.1 1,948,383 - SO1857 0.17 +0.5 1,948,508 + SO1857 0.24 +2.8 1,948,579 - SO1857 0.28 +1.4 1,948,579 - SO1857 0.28 +0.2 1,948,624 - SO1857 0.31 -0.2 1,948,645 - SO1857 0.32 +0.3 1,948,759 + SO1857 0.39 -0.1 1,948,773 - SO1857 0.40 -0.6 1,948,773 - SO1857 0.40 +0.2 1,948,793 + SO1857 0.41 -1.8 1,948,809 - SO1857 0.42 -0.8 1,948,927 - SO1857 0.49 +0.5 1,948,968 - SO1857 0.51 +0.1 1,949,077 - SO1857 0.57 -0.0 1,949,077 - SO1857 0.57 +0.5 1,949,278 - SO1857 0.69 -0.2 1,949,328 + SO1857 0.72 +0.0 1,949,364 + SO1857 0.74 +2.0 1,949,444 + SO1857 0.78 +0.4 1,949,514 + SO1857 0.82 +0.9 1,949,744 - +2.3 1,949,771 + +0.3 1,949,832 + +0.4 1,949,933 - -0.4 1,949,980 - +0.1 1,950,604 - SO1858 0.75 -0.1 1,950,663 + SO1858 0.84 +0.2 1,950,668 + SO1858 0.85 +0.0 1,950,676 - SO1858 0.87 +0.5 1,950,695 + SO1858 0.90 +1.8 1,950,782 - +0.0 1,950,784 + -0.6 1,950,784 + -0.6 1,950,792 - -0.1
Or see this region's nucleotide sequence