Experiment: L-Lysine 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pspA and pspB are separated by 17 nucleotides pspB and pspC overlap by 14 nucleotides pspC and SO1810 are separated by 114 nucleotides
SO1807: pspA - phage shock protein A (NCBI ptt file), at 1,897,828 to 1,898,511
pspA
SO1808: pspB - phage shock protein B (NCBI ptt file), at 1,898,529 to 1,898,768
pspB
SO1809: pspC - phage shock protein C (NCBI ptt file), at 1,898,755 to 1,899,141
pspC
SO1810: SO1810 - hypothetical ATPase (NCBI ptt file), at 1,899,256 to 1,900,713
SO1810
Position (kb)
1898
1899
1900 Strain fitness (log2 ratio)
-2
-1
0
1 at 1897.857 kb on + strand at 1897.863 kb on + strand at 1897.891 kb on + strand at 1897.947 kb on + strand, within pspA at 1898.142 kb on - strand, within pspA at 1898.253 kb on + strand, within pspA at 1898.512 kb on + strand at 1898.707 kb on - strand, within pspB at 1898.830 kb on + strand, within pspC at 1898.830 kb on + strand, within pspC at 1898.838 kb on - strand, within pspC at 1898.941 kb on - strand, within pspC at 1898.961 kb on - strand, within pspC at 1898.999 kb on - strand, within pspC at 1899.059 kb on - strand, within pspC at 1899.481 kb on - strand, within SO1810 at 1899.497 kb on - strand, within SO1810 at 1899.587 kb on - strand, within SO1810 at 1899.589 kb on + strand, within SO1810 at 1899.756 kb on - strand, within SO1810 at 1900.005 kb on + strand, within SO1810 at 1900.034 kb on + strand, within SO1810 at 1900.042 kb on - strand, within SO1810 at 1900.042 kb on - strand, within SO1810
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 100 mM remove 1,897,857 + -0.5 1,897,863 + -0.8 1,897,891 + +0.0 1,897,947 + pspA SO1807 0.17 +0.7 1,898,142 - pspA SO1807 0.46 +0.2 1,898,253 + pspA SO1807 0.62 +0.1 1,898,512 + +0.8 1,898,707 - pspB SO1808 0.74 -0.6 1,898,830 + pspC SO1809 0.19 -2.1 1,898,830 + pspC SO1809 0.19 +0.2 1,898,838 - pspC SO1809 0.21 -2.7 1,898,941 - pspC SO1809 0.48 -0.3 1,898,961 - pspC SO1809 0.53 -0.9 1,898,999 - pspC SO1809 0.63 -0.6 1,899,059 - pspC SO1809 0.79 -0.3 1,899,481 - SO1810 0.15 -0.0 1,899,497 - SO1810 0.17 -0.1 1,899,587 - SO1810 0.23 -0.7 1,899,589 + SO1810 0.23 +0.8 1,899,756 - SO1810 0.34 -1.3 1,900,005 + SO1810 0.51 +0.8 1,900,034 + SO1810 0.53 +1.0 1,900,042 - SO1810 0.54 +0.3 1,900,042 - SO1810 0.54 -0.4
Or see this region's nucleotide sequence