Experiment: L-Lysine 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt era and recO are separated by 54 nucleotides recO and pdxJ are separated by 84 nucleotides pdxJ and acpS overlap by 1 nucleotides
SO1349: era - GTP-binding protein Era (NCBI ptt file), at 1,405,603 to 1,406,622
era
SO1350: recO - DNA repair protein RecO (NCBI ptt file), at 1,406,677 to 1,407,378
recO
SO1351: pdxJ - pyridoxal phosphate biosynthetic protein PdxJ (NCBI ptt file), at 1,407,463 to 1,408,200
pdxJ
SO1352: acpS - holo-(acyl-carrier protein) synthase (NCBI ptt file), at 1,408,200 to 1,408,583
acpS
Position (kb)
1406
1407
1408 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1406.608 kb on - strand at 1406.637 kb on - strand at 1406.643 kb on + strand at 1406.651 kb on - strand at 1406.685 kb on + strand at 1406.692 kb on + strand at 1406.981 kb on - strand, within recO at 1407.049 kb on - strand, within recO at 1407.124 kb on + strand, within recO at 1407.126 kb on + strand, within recO at 1407.131 kb on + strand, within recO at 1407.314 kb on + strand at 1407.360 kb on - strand at 1407.410 kb on + strand at 1407.410 kb on + strand at 1407.410 kb on + strand at 1407.410 kb on + strand at 1407.418 kb on - strand at 1408.297 kb on - strand, within acpS
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 100 mM remove 1,406,608 - -0.5 1,406,637 - -1.6 1,406,643 + -0.0 1,406,651 - -1.9 1,406,685 + -0.5 1,406,692 + -0.6 1,406,981 - recO SO1350 0.43 -0.8 1,407,049 - recO SO1350 0.53 -1.1 1,407,124 + recO SO1350 0.64 -1.1 1,407,126 + recO SO1350 0.64 -1.7 1,407,131 + recO SO1350 0.65 -0.5 1,407,314 + +0.2 1,407,360 - +2.0 1,407,410 + -0.4 1,407,410 + -0.4 1,407,410 + -0.5 1,407,410 + -0.3 1,407,418 - -0.8 1,408,297 - acpS SO1352 0.25 -0.1
Or see this region's nucleotide sequence