Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapE and SO2472 are separated by 5 nucleotides SO2472 and SO2473 are separated by 69 nucleotides SO2473 and SO2474 are separated by 168 nucleotides
SO2471: dapE - succinyl-diaminopimelate desuccinylase (NCBI ptt file), at 2,593,058 to 2,594,212
dapE
SO2472: SO2472 - D-alanyl-D-alanine carboxypeptidase-related protein (NCBI ptt file), at 2,594,218 to 2,594,976
SO2472
SO2473: SO2473 - hydrolase, alpha/beta fold family (NCBI ptt file), at 2,595,046 to 2,595,879
SO2473
SO2474: SO2474 - carbonic anhydrase family protein (NCBI ptt file), at 2,596,048 to 2,596,653
SO2474
Position (kb)
2595
2596 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2594.244 kb on + strand at 2594.257 kb on + strand at 2594.257 kb on + strand at 2594.342 kb on + strand, within SO2472 at 2594.801 kb on + strand, within SO2472 at 2594.801 kb on + strand, within SO2472 at 2594.970 kb on + strand at 2595.096 kb on + strand at 2595.146 kb on + strand, within SO2473 at 2595.148 kb on + strand, within SO2473 at 2595.290 kb on + strand, within SO2473 at 2595.458 kb on + strand, within SO2473 at 2595.518 kb on - strand, within SO2473 at 2595.597 kb on - strand, within SO2473 at 2595.657 kb on + strand, within SO2473 at 2595.703 kb on + strand, within SO2473 at 2595.706 kb on - strand, within SO2473 at 2595.721 kb on - strand, within SO2473 at 2595.734 kb on - strand, within SO2473 at 2595.858 kb on - strand at 2595.908 kb on + strand at 2596.158 kb on + strand, within SO2474 at 2596.347 kb on - strand, within SO2474 at 2596.573 kb on + strand, within SO2474 at 2596.726 kb on - strand at 2596.848 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 2,594,244 + -2.7 2,594,257 + -1.6 2,594,257 + -3.6 2,594,342 + SO2472 0.16 -2.1 2,594,801 + SO2472 0.77 -3.3 2,594,801 + SO2472 0.77 -2.3 2,594,970 + +0.2 2,595,096 + +0.3 2,595,146 + SO2473 0.12 -0.6 2,595,148 + SO2473 0.12 -0.2 2,595,290 + SO2473 0.29 +1.5 2,595,458 + SO2473 0.49 -1.1 2,595,518 - SO2473 0.57 -0.2 2,595,597 - SO2473 0.66 +0.5 2,595,657 + SO2473 0.73 -0.5 2,595,703 + SO2473 0.79 +0.6 2,595,706 - SO2473 0.79 +1.0 2,595,721 - SO2473 0.81 +0.8 2,595,734 - SO2473 0.82 -0.1 2,595,858 - -1.1 2,595,908 + +0.6 2,596,158 + SO2474 0.18 +0.5 2,596,347 - SO2474 0.49 +0.4 2,596,573 + SO2474 0.87 -1.3 2,596,726 - +0.2 2,596,848 - -0.3
Or see this region's nucleotide sequence