Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0960 and SO0961 are separated by 183 nucleotides SO0961 and SO0962 are separated by 245 nucleotides SO0962 and SO0963 are separated by 213 nucleotides SO0963 and SO0964 are separated by 143 nucleotides SO0964 and SO0965 are separated by 60 nucleotides
SO0960: SO0960 - hypothetical spermidine synthase (NCBI ptt file), at 995,569 to 996,312
SO0960
SO0961: SO0961 - conserved hypothetical protein (NCBI ptt file), at 996,496 to 996,948
SO0961
SO0962: SO0962 - conserved hypothetical protein (NCBI ptt file), at 997,194 to 997,718
SO0962
SO0963: SO0963 - conserved hypothetical protein (NCBI ptt file), at 997,932 to 998,162
SO0963
SO0964: SO0964 - conserved hypothetical protein (NCBI ptt file), at 998,306 to 998,512
SO0964
SO0965: SO0965 - ISSod2, transposase OrfB (NCBI ptt file), at 998,573 to 999,388
SO0965
Position (kb) 997
998 Strain fitness (log2 ratio) -1
0
1 at 996.227 kb on - strand, within SO0960 at 996.245 kb on + strand at 996.267 kb on + strand at 996.275 kb on - strand at 996.362 kb on - strand at 996.464 kb on + strand at 996.471 kb on - strand at 996.472 kb on - strand at 996.504 kb on + strand at 996.504 kb on + strand at 996.504 kb on + strand at 996.507 kb on - strand at 996.512 kb on - strand at 996.512 kb on - strand at 996.512 kb on - strand at 996.512 kb on - strand at 996.512 kb on - strand at 996.572 kb on + strand, within SO0961 at 996.587 kb on + strand, within SO0961 at 996.595 kb on - strand, within SO0961 at 996.602 kb on + strand, within SO0961 at 996.650 kb on - strand, within SO0961 at 996.668 kb on + strand, within SO0961 at 996.676 kb on - strand, within SO0961 at 996.676 kb on - strand, within SO0961 at 996.731 kb on - strand, within SO0961 at 996.731 kb on - strand, within SO0961 at 996.733 kb on - strand, within SO0961 at 996.758 kb on - strand, within SO0961 at 996.765 kb on - strand, within SO0961 at 996.824 kb on - strand, within SO0961 at 996.979 kb on - strand at 997.141 kb on - strand at 997.164 kb on + strand at 997.332 kb on + strand, within SO0962 at 997.346 kb on + strand, within SO0962 at 997.346 kb on + strand, within SO0962 at 997.356 kb on + strand, within SO0962 at 997.356 kb on + strand, within SO0962 at 997.356 kb on + strand, within SO0962 at 997.356 kb on + strand, within SO0962 at 997.359 kb on - strand, within SO0962 at 997.364 kb on - strand, within SO0962 at 997.366 kb on - strand, within SO0962 at 997.459 kb on - strand, within SO0962 at 997.486 kb on + strand, within SO0962 at 997.505 kb on + strand, within SO0962 at 997.505 kb on + strand, within SO0962 at 997.513 kb on - strand, within SO0962 at 997.558 kb on + strand, within SO0962 at 997.625 kb on + strand, within SO0962 at 997.686 kb on - strand at 997.686 kb on - strand at 997.698 kb on + strand at 997.706 kb on - strand at 997.706 kb on - strand at 997.740 kb on - strand at 997.754 kb on - strand at 997.769 kb on + strand at 997.777 kb on - strand at 997.793 kb on - strand at 997.895 kb on + strand at 998.027 kb on + strand, within SO0963 at 998.034 kb on - strand, within SO0963 at 998.039 kb on + strand, within SO0963 at 998.063 kb on + strand, within SO0963 at 998.071 kb on - strand, within SO0963 at 998.071 kb on - strand, within SO0963 at 998.081 kb on + strand, within SO0963 at 998.123 kb on - strand, within SO0963 at 998.188 kb on + strand at 998.201 kb on - strand at 998.201 kb on - strand at 998.207 kb on - strand at 998.214 kb on - strand at 998.244 kb on + strand at 998.244 kb on + strand at 998.252 kb on - strand at 998.273 kb on + strand at 998.297 kb on - strand at 998.309 kb on + strand at 998.356 kb on - strand, within SO0964 at 998.364 kb on - strand, within SO0964 at 998.416 kb on + strand, within SO0964 at 998.474 kb on + strand, within SO0964 at 998.481 kb on + strand, within SO0964 at 998.489 kb on - strand, within SO0964
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 996,227 - SO0960 0.88 -0.4 996,245 + +0.6 996,267 + +0.4 996,275 - -0.4 996,362 - +0.2 996,464 + +0.1 996,471 - -0.3 996,472 - +0.4 996,504 + -0.7 996,504 + +0.1 996,504 + +1.1 996,507 - -0.5 996,512 - -0.9 996,512 - -0.1 996,512 - -0.4 996,512 - -0.2 996,512 - +0.4 996,572 + SO0961 0.17 -1.6 996,587 + SO0961 0.20 -0.2 996,595 - SO0961 0.22 -0.4 996,602 + SO0961 0.23 +0.0 996,650 - SO0961 0.34 +0.6 996,668 + SO0961 0.38 +0.1 996,676 - SO0961 0.40 -0.1 996,676 - SO0961 0.40 -0.0 996,731 - SO0961 0.52 +1.1 996,731 - SO0961 0.52 +0.2 996,733 - SO0961 0.52 +0.2 996,758 - SO0961 0.58 -1.2 996,765 - SO0961 0.59 -1.4 996,824 - SO0961 0.72 +0.4 996,979 - -0.0 997,141 - +0.0 997,164 + -0.4 997,332 + SO0962 0.26 +0.2 997,346 + SO0962 0.29 +0.7 997,346 + SO0962 0.29 +0.6 997,356 + SO0962 0.31 +0.3 997,356 + SO0962 0.31 +0.4 997,356 + SO0962 0.31 +0.2 997,356 + SO0962 0.31 +1.3 997,359 - SO0962 0.31 -0.4 997,364 - SO0962 0.32 +0.3 997,366 - SO0962 0.33 -1.5 997,459 - SO0962 0.50 +0.3 997,486 + SO0962 0.56 +1.0 997,505 + SO0962 0.59 +0.1 997,505 + SO0962 0.59 +0.8 997,513 - SO0962 0.61 -0.4 997,558 + SO0962 0.69 +0.3 997,625 + SO0962 0.82 +0.2 997,686 - -0.3 997,686 - -0.3 997,698 + +0.6 997,706 - -0.1 997,706 - +0.2 997,740 - -0.1 997,754 - -0.2 997,769 + +0.5 997,777 - +0.7 997,793 - +0.5 997,895 + +0.3 998,027 + SO0963 0.41 -0.8 998,034 - SO0963 0.44 -1.0 998,039 + SO0963 0.46 +0.3 998,063 + SO0963 0.57 -0.0 998,071 - SO0963 0.60 -1.0 998,071 - SO0963 0.60 -0.1 998,081 + SO0963 0.65 -0.9 998,123 - SO0963 0.83 -0.5 998,188 + -0.3 998,201 - +0.1 998,201 - -0.4 998,207 - -0.5 998,214 - +0.6 998,244 + +0.3 998,244 + -0.4 998,252 - +0.2 998,273 + -0.7 998,297 - -0.5 998,309 + -0.2 998,356 - SO0964 0.24 +0.1 998,364 - SO0964 0.28 +0.1 998,416 + SO0964 0.53 +0.0 998,474 + SO0964 0.81 +0.4 998,481 + SO0964 0.85 +0.4 998,489 - SO0964 0.88 +0.1
Or see this region's nucleotide sequence