Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amiB and mutL are separated by 13 nucleotides mutL and miaA overlap by 8 nucleotides
SO0600: amiB - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 625,445 to 626,836
amiB
SO0601: mutL - DNA mismatch repair protein MutL (NCBI ptt file), at 626,850 to 628,745
mutL
SO0602: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI ptt file), at 628,738 to 629,664
miaA
Position (kb) 626
627
628
629 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 625.865 kb on - strand, within amiB at 625.886 kb on - strand, within amiB at 626.364 kb on + strand, within amiB at 626.364 kb on + strand, within amiB at 626.364 kb on + strand, within amiB at 626.364 kb on + strand, within amiB at 626.431 kb on + strand, within amiB at 626.663 kb on + strand, within amiB at 626.898 kb on + strand at 626.920 kb on + strand at 626.958 kb on - strand at 626.964 kb on - strand at 626.998 kb on + strand at 626.998 kb on + strand at 627.062 kb on - strand, within mutL at 627.102 kb on - strand, within mutL at 627.104 kb on + strand, within mutL at 627.112 kb on - strand, within mutL at 627.190 kb on + strand, within mutL at 627.190 kb on + strand, within mutL at 627.198 kb on - strand, within mutL at 627.200 kb on + strand, within mutL at 627.280 kb on + strand, within mutL at 627.370 kb on + strand, within mutL at 627.384 kb on + strand, within mutL at 627.546 kb on + strand, within mutL at 627.588 kb on + strand, within mutL at 627.588 kb on + strand, within mutL at 627.588 kb on - strand, within mutL at 627.708 kb on - strand, within mutL at 627.912 kb on - strand, within mutL at 627.972 kb on + strand, within mutL at 627.972 kb on + strand, within mutL at 627.975 kb on + strand, within mutL at 627.978 kb on - strand, within mutL at 628.050 kb on + strand, within mutL at 628.070 kb on - strand, within mutL at 628.126 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.212 kb on - strand, within mutL at 628.529 kb on + strand, within mutL at 628.537 kb on - strand, within mutL at 628.537 kb on - strand, within mutL at 628.695 kb on + strand at 628.891 kb on + strand, within miaA at 629.010 kb on + strand, within miaA at 629.302 kb on + strand, within miaA at 629.395 kb on + strand, within miaA at 629.471 kb on + strand, within miaA
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 625,865 - amiB SO0600 0.30 -1.8 625,886 - amiB SO0600 0.32 -1.7 626,364 + amiB SO0600 0.66 -3.5 626,364 + amiB SO0600 0.66 +0.5 626,364 + amiB SO0600 0.66 -1.8 626,364 + amiB SO0600 0.66 -2.4 626,431 + amiB SO0600 0.71 -4.0 626,663 + amiB SO0600 0.88 -3.6 626,898 + -0.6 626,920 + +0.6 626,958 - -0.8 626,964 - +1.4 626,998 + -0.2 626,998 + +1.1 627,062 - mutL SO0601 0.11 -0.5 627,102 - mutL SO0601 0.13 +0.3 627,104 + mutL SO0601 0.13 +0.0 627,112 - mutL SO0601 0.14 -0.8 627,190 + mutL SO0601 0.18 -0.4 627,190 + mutL SO0601 0.18 -0.9 627,198 - mutL SO0601 0.18 +0.4 627,200 + mutL SO0601 0.18 +0.8 627,280 + mutL SO0601 0.23 -1.2 627,370 + mutL SO0601 0.27 +0.2 627,384 + mutL SO0601 0.28 +1.0 627,546 + mutL SO0601 0.37 -0.0 627,588 + mutL SO0601 0.39 -0.3 627,588 + mutL SO0601 0.39 -0.4 627,588 - mutL SO0601 0.39 +0.0 627,708 - mutL SO0601 0.45 +0.4 627,912 - mutL SO0601 0.56 -0.2 627,972 + mutL SO0601 0.59 +0.0 627,972 + mutL SO0601 0.59 -1.6 627,975 + mutL SO0601 0.59 -0.2 627,978 - mutL SO0601 0.59 -0.5 628,050 + mutL SO0601 0.63 -0.3 628,070 - mutL SO0601 0.64 +0.8 628,126 + mutL SO0601 0.67 -0.2 628,199 + mutL SO0601 0.71 -0.1 628,199 + mutL SO0601 0.71 +0.1 628,199 + mutL SO0601 0.71 -0.2 628,199 + mutL SO0601 0.71 -0.7 628,199 + mutL SO0601 0.71 -0.2 628,199 + mutL SO0601 0.71 +0.0 628,199 + mutL SO0601 0.71 -0.6 628,199 + mutL SO0601 0.71 +0.1 628,207 - mutL SO0601 0.72 -0.9 628,207 - mutL SO0601 0.72 +0.5 628,207 - mutL SO0601 0.72 -1.1 628,207 - mutL SO0601 0.72 -0.4 628,207 - mutL SO0601 0.72 +0.3 628,207 - mutL SO0601 0.72 -0.6 628,207 - mutL SO0601 0.72 +0.2 628,207 - mutL SO0601 0.72 -0.1 628,207 - mutL SO0601 0.72 -0.6 628,207 - mutL SO0601 0.72 -1.8 628,207 - mutL SO0601 0.72 -0.4 628,207 - mutL SO0601 0.72 -0.5 628,207 - mutL SO0601 0.72 -0.1 628,212 - mutL SO0601 0.72 -0.4 628,529 + mutL SO0601 0.89 -2.6 628,537 - mutL SO0601 0.89 -2.7 628,537 - mutL SO0601 0.89 -0.5 628,695 + -1.5 628,891 + miaA SO0602 0.17 -1.5 629,010 + miaA SO0602 0.29 -1.9 629,302 + miaA SO0602 0.61 -1.6 629,395 + miaA SO0602 0.71 -0.2 629,471 + miaA SO0602 0.79 -1.3
Or see this region's nucleotide sequence