Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3185 and rfbA overlap by 4 nucleotides rfbA and rfbB are separated by 454 nucleotides
SO3185: SO3185 - polysaccharide biosynthesis protein (NCBI ptt file), at 3,323,470 to 3,324,546
SO3185
SO3186: rfbA - glucose-1-phosphate-thymidylyltransferase (NCBI ptt file), at 3,324,543 to 3,325,457
rfbA
SO3188: rfbB - dTDP-glucose 4,6-dehydratase (NCBI ptt file), at 3,325,912 to 3,327,039
rfbB
Position (kb)
3324
3325
3326 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3323.543 kb on - strand at 3323.545 kb on - strand at 3323.545 kb on - strand at 3323.545 kb on - strand at 3323.551 kb on + strand at 3323.587 kb on + strand, within SO3185 at 3323.587 kb on + strand, within SO3185 at 3323.587 kb on + strand, within SO3185 at 3323.589 kb on - strand, within SO3185 at 3323.595 kb on - strand, within SO3185 at 3323.595 kb on - strand, within SO3185 at 3323.599 kb on + strand, within SO3185 at 3323.607 kb on - strand, within SO3185 at 3323.607 kb on - strand, within SO3185 at 3323.607 kb on - strand, within SO3185 at 3323.607 kb on - strand, within SO3185 at 3323.614 kb on - strand, within SO3185 at 3323.614 kb on - strand, within SO3185 at 3323.648 kb on - strand, within SO3185 at 3323.681 kb on - strand, within SO3185 at 3323.767 kb on + strand, within SO3185 at 3323.796 kb on + strand, within SO3185 at 3323.796 kb on + strand, within SO3185 at 3323.796 kb on + strand, within SO3185 at 3323.804 kb on - strand, within SO3185 at 3323.804 kb on - strand, within SO3185 at 3323.804 kb on - strand, within SO3185 at 3323.847 kb on - strand, within SO3185 at 3323.847 kb on - strand, within SO3185 at 3323.847 kb on - strand, within SO3185 at 3323.847 kb on - strand, within SO3185 at 3323.852 kb on - strand, within SO3185 at 3323.873 kb on - strand at 3323.891 kb on + strand, within SO3185 at 3323.899 kb on - strand, within SO3185 at 3323.899 kb on - strand, within SO3185 at 3323.899 kb on - strand, within SO3185 at 3323.975 kb on + strand, within SO3185 at 3323.980 kb on + strand, within SO3185 at 3323.980 kb on + strand, within SO3185 at 3323.980 kb on + strand, within SO3185 at 3323.980 kb on + strand, within SO3185 at 3323.980 kb on + strand, within SO3185 at 3323.980 kb on + strand, within SO3185 at 3323.980 kb on + strand, within SO3185 at 3323.983 kb on - strand, within SO3185 at 3323.983 kb on - strand, within SO3185 at 3323.988 kb on - strand, within SO3185 at 3323.988 kb on - strand, within SO3185 at 3323.988 kb on - strand, within SO3185 at 3323.988 kb on - strand, within SO3185 at 3323.988 kb on - strand, within SO3185 at 3323.988 kb on - strand, within SO3185 at 3323.988 kb on - strand, within SO3185 at 3323.988 kb on - strand, within SO3185 at 3323.988 kb on - strand, within SO3185 at 3324.024 kb on - strand, within SO3185 at 3324.048 kb on - strand, within SO3185 at 3324.094 kb on - strand, within SO3185 at 3324.096 kb on - strand, within SO3185 at 3324.154 kb on + strand, within SO3185 at 3324.155 kb on + strand, within SO3185 at 3324.155 kb on + strand, within SO3185 at 3324.162 kb on - strand, within SO3185 at 3324.164 kb on + strand, within SO3185 at 3324.170 kb on - strand, within SO3185 at 3324.170 kb on - strand, within SO3185 at 3324.172 kb on - strand, within SO3185 at 3324.172 kb on - strand, within SO3185 at 3324.179 kb on - strand, within SO3185 at 3324.188 kb on - strand, within SO3185 at 3324.189 kb on - strand, within SO3185 at 3324.219 kb on + strand at 3324.227 kb on - strand, within SO3185 at 3324.227 kb on - strand, within SO3185 at 3324.237 kb on + strand, within SO3185 at 3324.244 kb on + strand, within SO3185 at 3324.244 kb on + strand, within SO3185 at 3324.244 kb on - strand, within SO3185 at 3324.244 kb on - strand, within SO3185 at 3324.252 kb on - strand, within SO3185 at 3324.252 kb on - strand, within SO3185 at 3324.252 kb on - strand, within SO3185 at 3324.267 kb on + strand, within SO3185 at 3324.268 kb on + strand, within SO3185 at 3324.274 kb on - strand, within SO3185 at 3324.275 kb on - strand, within SO3185 at 3324.275 kb on - strand, within SO3185 at 3324.276 kb on - strand, within SO3185 at 3324.279 kb on + strand, within SO3185 at 3324.291 kb on + strand, within SO3185 at 3324.316 kb on - strand, within SO3185 at 3324.341 kb on - strand, within SO3185 at 3324.346 kb on - strand, within SO3185 at 3324.356 kb on - strand, within SO3185 at 3324.383 kb on - strand, within SO3185 at 3324.391 kb on - strand, within SO3185 at 3324.408 kb on - strand, within SO3185 at 3324.413 kb on - strand, within SO3185 at 3324.415 kb on - strand, within SO3185 at 3324.415 kb on - strand, within SO3185 at 3324.415 kb on - strand, within SO3185 at 3324.415 kb on - strand, within SO3185 at 3324.415 kb on - strand, within SO3185 at 3324.415 kb on - strand, within SO3185 at 3324.415 kb on - strand, within SO3185 at 3324.415 kb on - strand, within SO3185 at 3324.415 kb on - strand, within SO3185 at 3324.439 kb on + strand at 3324.439 kb on + strand at 3324.443 kb on - strand at 3324.447 kb on - strand at 3324.447 kb on - strand at 3324.463 kb on + strand at 3324.468 kb on + strand at 3324.471 kb on - strand at 3324.471 kb on - strand at 3324.486 kb on + strand at 3324.486 kb on + strand at 3324.486 kb on + strand at 3324.493 kb on - strand at 3324.494 kb on - strand at 3324.494 kb on - strand at 3324.494 kb on - strand at 3324.497 kb on - strand at 3324.497 kb on - strand at 3324.640 kb on + strand, within rfbA at 3324.673 kb on + strand, within rfbA at 3324.718 kb on + strand, within rfbA at 3324.718 kb on + strand, within rfbA at 3324.718 kb on + strand, within rfbA at 3324.726 kb on - strand, within rfbA at 3324.726 kb on - strand, within rfbA at 3324.726 kb on - strand, within rfbA at 3324.726 kb on - strand, within rfbA at 3324.745 kb on + strand, within rfbA at 3324.753 kb on - strand, within rfbA at 3324.753 kb on - strand, within rfbA at 3324.812 kb on + strand, within rfbA at 3324.820 kb on - strand, within rfbA at 3324.849 kb on + strand, within rfbA at 3324.894 kb on + strand, within rfbA at 3325.000 kb on + strand, within rfbA at 3325.008 kb on - strand, within rfbA at 3325.014 kb on + strand, within rfbA at 3325.022 kb on - strand, within rfbA at 3325.022 kb on - strand, within rfbA at 3325.030 kb on - strand, within rfbA at 3325.040 kb on - strand, within rfbA at 3325.108 kb on - strand, within rfbA at 3325.139 kb on - strand, within rfbA at 3325.169 kb on - strand, within rfbA at 3325.169 kb on - strand, within rfbA at 3325.173 kb on + strand, within rfbA at 3325.173 kb on + strand, within rfbA at 3325.173 kb on + strand, within rfbA at 3325.191 kb on - strand, within rfbA at 3325.191 kb on - strand, within rfbA at 3325.248 kb on - strand, within rfbA at 3325.290 kb on + strand, within rfbA at 3325.298 kb on - strand, within rfbA at 3325.298 kb on - strand, within rfbA at 3325.334 kb on - strand, within rfbA at 3325.336 kb on + strand, within rfbA at 3325.344 kb on - strand, within rfbA at 3325.400 kb on + strand at 3325.444 kb on - strand at 3325.445 kb on - strand at 3325.478 kb on + strand at 3325.486 kb on - strand at 3325.540 kb on + strand at 3325.548 kb on - strand at 3325.548 kb on - strand at 3325.556 kb on - strand at 3325.570 kb on - strand at 3325.570 kb on - strand at 3325.576 kb on + strand at 3325.579 kb on + strand at 3325.659 kb on + strand at 3325.659 kb on + strand at 3325.659 kb on + strand at 3325.659 kb on + strand at 3325.659 kb on + strand at 3325.659 kb on + strand at 3325.659 kb on + strand at 3325.659 kb on + strand at 3325.659 kb on + strand at 3325.667 kb on - strand at 3325.667 kb on - strand at 3325.667 kb on - strand at 3325.667 kb on - strand at 3325.667 kb on - strand at 3325.667 kb on - strand at 3325.667 kb on - strand at 3325.667 kb on - strand at 3325.667 kb on - strand at 3325.667 kb on - strand at 3325.667 kb on - strand at 3325.667 kb on - strand at 3325.669 kb on + strand at 3325.669 kb on + strand at 3325.676 kb on - strand at 3325.677 kb on - strand at 3325.781 kb on + strand at 3325.877 kb on + strand at 3325.905 kb on - strand at 3325.905 kb on - strand at 3325.917 kb on - strand at 3325.917 kb on - strand at 3325.917 kb on - strand at 3325.917 kb on - strand at 3325.927 kb on + strand at 3325.938 kb on + strand at 3325.938 kb on + strand at 3325.946 kb on - strand at 3325.946 kb on - strand at 3325.979 kb on + strand at 3325.979 kb on + strand at 3325.987 kb on - strand at 3325.997 kb on + strand at 3326.002 kb on + strand at 3326.005 kb on - strand at 3326.056 kb on - strand, within rfbB at 3326.395 kb on - strand, within rfbB
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM remove 3,323,543 - -0.3 3,323,545 - -1.2 3,323,545 - +0.0 3,323,545 - -2.3 3,323,551 + -0.4 3,323,587 + SO3185 0.11 -1.3 3,323,587 + SO3185 0.11 +0.0 3,323,587 + SO3185 0.11 -0.3 3,323,589 - SO3185 0.11 -1.1 3,323,595 - SO3185 0.12 -0.5 3,323,595 - SO3185 0.12 +0.4 3,323,599 + SO3185 0.12 +0.5 3,323,607 - SO3185 0.13 -1.2 3,323,607 - SO3185 0.13 -0.2 3,323,607 - SO3185 0.13 -0.3 3,323,607 - SO3185 0.13 -1.3 3,323,614 - SO3185 0.13 -0.4 3,323,614 - SO3185 0.13 -0.7 3,323,648 - SO3185 0.17 -0.9 3,323,681 - SO3185 0.20 -1.1 3,323,767 + SO3185 0.28 -0.7 3,323,796 + SO3185 0.30 -0.7 3,323,796 + SO3185 0.30 +1.0 3,323,796 + SO3185 0.30 -0.4 3,323,804 - SO3185 0.31 -0.7 3,323,804 - SO3185 0.31 +0.5 3,323,804 - SO3185 0.31 -1.0 3,323,847 - SO3185 0.35 -0.9 3,323,847 - SO3185 0.35 -0.9 3,323,847 - SO3185 0.35 -0.5 3,323,847 - SO3185 0.35 -0.8 3,323,852 - SO3185 0.35 -0.2 3,323,873 - +0.4 3,323,891 + SO3185 0.39 -0.8 3,323,899 - SO3185 0.40 -3.6 3,323,899 - SO3185 0.40 +0.6 3,323,899 - SO3185 0.40 -0.2 3,323,975 + SO3185 0.47 +0.1 3,323,980 + SO3185 0.47 +0.7 3,323,980 + SO3185 0.47 +0.0 3,323,980 + SO3185 0.47 -0.5 3,323,980 + SO3185 0.47 -0.9 3,323,980 + SO3185 0.47 +0.9 3,323,980 + SO3185 0.47 -0.8 3,323,980 + SO3185 0.47 +1.1 3,323,983 - SO3185 0.48 +0.1 3,323,983 - SO3185 0.48 -0.3 3,323,988 - SO3185 0.48 -1.1 3,323,988 - SO3185 0.48 +0.7 3,323,988 - SO3185 0.48 -1.1 3,323,988 - SO3185 0.48 +0.4 3,323,988 - SO3185 0.48 -1.0 3,323,988 - SO3185 0.48 -1.7 3,323,988 - SO3185 0.48 -2.0 3,323,988 - SO3185 0.48 -0.1 3,323,988 - SO3185 0.48 +0.6 3,324,024 - SO3185 0.51 -0.9 3,324,048 - SO3185 0.54 -2.1 3,324,094 - SO3185 0.58 -0.7 3,324,096 - SO3185 0.58 -1.7 3,324,154 + SO3185 0.64 -0.3 3,324,155 + SO3185 0.64 -0.3 3,324,155 + SO3185 0.64 +0.6 3,324,162 - SO3185 0.64 -0.2 3,324,164 + SO3185 0.64 -1.2 3,324,170 - SO3185 0.65 -0.5 3,324,170 - SO3185 0.65 +1.1 3,324,172 - SO3185 0.65 -1.3 3,324,172 - SO3185 0.65 +0.7 3,324,179 - SO3185 0.66 +0.7 3,324,188 - SO3185 0.67 -1.4 3,324,189 - SO3185 0.67 -0.7 3,324,219 + +1.1 3,324,227 - SO3185 0.70 -0.0 3,324,227 - SO3185 0.70 -1.3 3,324,237 + SO3185 0.71 -1.0 3,324,244 + SO3185 0.72 -0.4 3,324,244 + SO3185 0.72 -1.0 3,324,244 - SO3185 0.72 -1.2 3,324,244 - SO3185 0.72 -0.3 3,324,252 - SO3185 0.73 -0.1 3,324,252 - SO3185 0.73 -0.8 3,324,252 - SO3185 0.73 -0.9 3,324,267 + SO3185 0.74 -1.0 3,324,268 + SO3185 0.74 -0.6 3,324,274 - SO3185 0.75 -1.1 3,324,275 - SO3185 0.75 -1.0 3,324,275 - SO3185 0.75 -0.2 3,324,276 - SO3185 0.75 -0.7 3,324,279 + SO3185 0.75 +0.1 3,324,291 + SO3185 0.76 -0.1 3,324,316 - SO3185 0.79 +0.3 3,324,341 - SO3185 0.81 -0.6 3,324,346 - SO3185 0.81 -1.7 3,324,356 - SO3185 0.82 -1.4 3,324,383 - SO3185 0.85 -0.5 3,324,391 - SO3185 0.86 +0.2 3,324,408 - SO3185 0.87 -0.0 3,324,413 - SO3185 0.88 +1.1 3,324,415 - SO3185 0.88 -0.7 3,324,415 - SO3185 0.88 +1.1 3,324,415 - SO3185 0.88 -1.5 3,324,415 - SO3185 0.88 -0.4 3,324,415 - SO3185 0.88 -2.4 3,324,415 - SO3185 0.88 -0.4 3,324,415 - SO3185 0.88 -1.0 3,324,415 - SO3185 0.88 -0.0 3,324,415 - SO3185 0.88 -0.8 3,324,439 + +0.3 3,324,439 + -1.0 3,324,443 - -0.6 3,324,447 - -1.8 3,324,447 - -0.6 3,324,463 + -0.7 3,324,468 + +0.9 3,324,471 - -0.6 3,324,471 - -0.7 3,324,486 + +0.9 3,324,486 + -1.2 3,324,486 + -0.4 3,324,493 - +0.7 3,324,494 - -0.2 3,324,494 - +0.0 3,324,494 - -0.4 3,324,497 - +0.1 3,324,497 - -1.9 3,324,640 + rfbA SO3186 0.11 -0.4 3,324,673 + rfbA SO3186 0.14 -1.2 3,324,718 + rfbA SO3186 0.19 -0.6 3,324,718 + rfbA SO3186 0.19 -1.0 3,324,718 + rfbA SO3186 0.19 -1.6 3,324,726 - rfbA SO3186 0.20 -1.5 3,324,726 - rfbA SO3186 0.20 +0.1 3,324,726 - rfbA SO3186 0.20 -1.9 3,324,726 - rfbA SO3186 0.20 -1.1 3,324,745 + rfbA SO3186 0.22 -0.7 3,324,753 - rfbA SO3186 0.23 -1.1 3,324,753 - rfbA SO3186 0.23 -0.4 3,324,812 + rfbA SO3186 0.29 -1.1 3,324,820 - rfbA SO3186 0.30 -0.9 3,324,849 + rfbA SO3186 0.33 -2.1 3,324,894 + rfbA SO3186 0.38 -1.9 3,325,000 + rfbA SO3186 0.50 +1.4 3,325,008 - rfbA SO3186 0.51 -0.1 3,325,014 + rfbA SO3186 0.51 +1.0 3,325,022 - rfbA SO3186 0.52 +0.1 3,325,022 - rfbA SO3186 0.52 -1.7 3,325,030 - rfbA SO3186 0.53 -0.7 3,325,040 - rfbA SO3186 0.54 +0.3 3,325,108 - rfbA SO3186 0.62 -1.1 3,325,139 - rfbA SO3186 0.65 -0.6 3,325,169 - rfbA SO3186 0.68 -0.6 3,325,169 - rfbA SO3186 0.68 -1.7 3,325,173 + rfbA SO3186 0.69 -1.4 3,325,173 + rfbA SO3186 0.69 +0.9 3,325,173 + rfbA SO3186 0.69 -2.0 3,325,191 - rfbA SO3186 0.71 -1.6 3,325,191 - rfbA SO3186 0.71 -0.7 3,325,248 - rfbA SO3186 0.77 +0.1 3,325,290 + rfbA SO3186 0.82 -1.3 3,325,298 - rfbA SO3186 0.83 -0.5 3,325,298 - rfbA SO3186 0.83 +0.6 3,325,334 - rfbA SO3186 0.86 -1.0 3,325,336 + rfbA SO3186 0.87 -0.6 3,325,344 - rfbA SO3186 0.88 +0.0 3,325,400 + -1.4 3,325,444 - -0.4 3,325,445 - -1.4 3,325,478 + -0.7 3,325,486 - -0.6 3,325,540 + +1.2 3,325,548 - +0.1 3,325,548 - -1.2 3,325,556 - -0.2 3,325,570 - -0.6 3,325,570 - -0.7 3,325,576 + +0.6 3,325,579 + -0.5 3,325,659 + +0.6 3,325,659 + -0.8 3,325,659 + -1.1 3,325,659 + -0.3 3,325,659 + +0.2 3,325,659 + -0.1 3,325,659 + -0.1 3,325,659 + -0.6 3,325,659 + -1.3 3,325,667 - +0.4 3,325,667 - +0.1 3,325,667 - -0.9 3,325,667 - +0.1 3,325,667 - -0.1 3,325,667 - -0.1 3,325,667 - -0.0 3,325,667 - -0.8 3,325,667 - +0.3 3,325,667 - +0.4 3,325,667 - -0.1 3,325,667 - -0.6 3,325,669 + +1.2 3,325,669 + -0.5 3,325,676 - +0.4 3,325,677 - +0.2 3,325,781 + -2.0 3,325,877 + -1.6 3,325,905 - -0.8 3,325,905 - -0.2 3,325,917 - +0.5 3,325,917 - +0.1 3,325,917 - +1.3 3,325,917 - -0.4 3,325,927 + +0.0 3,325,938 + -0.2 3,325,938 + +0.2 3,325,946 - +0.9 3,325,946 - -0.5 3,325,979 + +0.3 3,325,979 + -0.3 3,325,987 - +0.1 3,325,997 + -0.1 3,326,002 + -0.5 3,326,005 - -0.9 3,326,056 - rfbB SO3188 0.13 -0.1 3,326,395 - rfbB SO3188 0.43 -0.2
Or see this region's nucleotide sequence